Cloned (Comment) | Organism |
---|---|
- |
Methylorubrum extorquens |
- |
Nostoc punctiforme |
- |
Cupriavidus metallidurans |
- |
Pseudomonas stutzeri |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant 17x-PTDH is based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+ | Methylorubrum extorquens |
additional information | mutant 17x-PTDH is based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+ | Nostoc punctiforme |
additional information | mutant 17x-PTDH is based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+ | Cupriavidus metallidurans |
additional information | mutant 17x-PTDH is based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+ | Pseudomonas stutzeri |
R301A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301A leads to sharp decrease in activity, while kcat value is similar to wild-type | Methylorubrum extorquens |
R301A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301A leads to sharp decrease in activity, while kcat value is similar to wild-type | Nostoc punctiforme |
R301A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301A leads to sharp decrease in activity, while kcat value is similar to wild-type | Cupriavidus metallidurans |
R301A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301A leads to sharp decrease in activity, while kcat value is similar to wild-type | Pseudomonas stutzeri |
R301K | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301K leads to sharp decrease in activity, while kcat value is similar to wild-type | Methylorubrum extorquens |
R301K | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301K leads to sharp decrease in activity, while kcat value is similar to wild-type | Nostoc punctiforme |
R301K | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301K leads to sharp decrease in activity, while kcat value is similar to wild-type | Cupriavidus metallidurans |
R301K | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301K leads to sharp decrease in activity, while kcat value is similar to wild-type | Pseudomonas stutzeri |
S295A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation S295A leads to sharp decrease in activity, while kcat value is similar to wild-type | Methylorubrum extorquens |
S295A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation S295A leads to sharp decrease in activity, while kcat value is similar to wild-type | Nostoc punctiforme |
S295A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation S295A leads to sharp decrease in activity, while kcat value is similar to wild-type | Cupriavidus metallidurans |
S295A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation S295A leads to sharp decrease in activity, while kcat value is similar to wild-type | Pseudomonas stutzeri |
W134A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134A leads to sharp decrease in activity, while kcat value is similar to wild-type | Methylorubrum extorquens |
W134A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134A leads to sharp decrease in activity, while kcat value is similar to wild-type | Nostoc punctiforme |
W134A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134A leads to sharp decrease in activity, while kcat value is similar to wild-type | Cupriavidus metallidurans |
W134A | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134A leads to sharp decrease in activity, while kcat value is similar to wild-type | Pseudomonas stutzeri |
W134F | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134F leads to sharp decrease in activity, while kcat value is similar to wild-type | Methylorubrum extorquens |
W134F | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134F leads to sharp decrease in activity, while kcat value is similar to wild-type | Nostoc punctiforme |
W134F | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134F leads to sharp decrease in activity, while kcat value is similar to wild-type | Cupriavidus metallidurans |
W134F | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134F leads to sharp decrease in activity, while kcat value is similar to wild-type | Pseudomonas stutzeri |
Y139F | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation Y139F leads to sharp decrease in activity, while kcat value is similar to wild-type | Methylorubrum extorquens |
Y139F | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation Y139F leads to sharp decrease in activity, while kcat value is similar to wild-type | Nostoc punctiforme |
Y139F | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation Y139F leads to sharp decrease in activity, while kcat value is similar to wild-type | Cupriavidus metallidurans |
Y139F | mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation Y139F leads to sharp decrease in activity, while kcat value is similar to wild-type | Pseudomonas stutzeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cupriavidus metallidurans | |
NAD+ | - |
Methylorubrum extorquens | |
NAD+ | - |
Nostoc punctiforme | |
NAD+ | - |
Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
NAD+ | pH 7.3, 25°C | Cupriavidus metallidurans | |
0.0194 | - |
NAD+ | pH 7.3, 25°C | Nostoc punctiforme | |
0.022 | - |
NAD+ | mutant 17X-PTDH, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.0254 | - |
phosphonate | pH 7.3, 25°C | Nostoc punctiforme | |
0.028 | - |
phosphonate | mutant 17X-PTDH, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.036 | - |
NAD+ | mutant Y139F, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.039 | - |
phosphonate | mutant Y139F, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.0405 | - |
NAD+ | pH 7.3, 25°C | Methylorubrum extorquens | |
0.0522 | - |
NAD+ | wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.0549 | - |
phosphonate | pH 7.3, 25°C | Cupriavidus metallidurans | |
0.074 | - |
NAD+ | mutant R301K, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.0811 | - |
phosphonate | wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.102 | - |
NAD+ | mutant W134F, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.103 | - |
phosphonate | mutant S295A, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.187 | - |
NAD+ | mutant W134A, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.216 | - |
NAD+ | mutant R301A, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.237 | - |
phosphonate | pH 7.3, 25°C | Methylorubrum extorquens | |
0.238 | - |
phosphonate | mutant W134F, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.25 | - |
NAD+ | mutant S295A, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.11 | - |
phosphonate | mutant R301K, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.91 | - |
phosphonate | mutant W134A, pH 7.3, 25°C | Pseudomonas stutzeri | |
19.4 | - |
phosphonate | mutant R301A, pH 7.3, 25°C | Pseudomonas stutzeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus metallidurans | - |
- |
- |
Cupriavidus metallidurans CH34 | - |
- |
- |
Methylorubrum extorquens | - |
- |
- |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | - |
- |
- |
Nostoc punctiforme | - |
- |
- |
Pseudomonas stutzeri | O69054 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonate + NAD+ + H2O | - |
Methylorubrum extorquens | phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | - |
Nostoc punctiforme | phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | - |
Cupriavidus metallidurans | phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | - |
Pseudomonas stutzeri | phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | - |
Cupriavidus metallidurans CH34 | phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PTDH | - |
Methylorubrum extorquens |
PTDH | - |
Nostoc punctiforme |
PTDH | - |
Cupriavidus metallidurans |
PTDH | - |
Pseudomonas stutzeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
phosphonate | mutant R301A, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.73 | - |
phosphonate | mutant W134A, pH 7.3, 25°C | Pseudomonas stutzeri | |
2.53 | - |
phosphonate | mutant S295A, pH 7.3, 25°C | Pseudomonas stutzeri | |
2.96 | - |
phosphonate | mutant W134F, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.27 | - |
phosphonate | mutant 17X-PTDH, pH 7.3, 25°C | Pseudomonas stutzeri | |
3.36 | - |
phosphonate | pH 7.3, 25°C | Nostoc punctiforme | |
3.68 | - |
phosphonate | mutant Y139F, pH 7.3, 25°C | Pseudomonas stutzeri | |
4.5 | - |
phosphonate | mutant R301K, pH 7.3, 25°C | Pseudomonas stutzeri | |
5.1 | - |
phosphonate | pH 7.3, 25°C | Methylorubrum extorquens | |
6.57 | - |
phosphonate | wild-type, pH 7.3, 25°C | Pseudomonas stutzeri | |
6.83 | - |
phosphonate | pH 7.3, 25°C | Cupriavidus metallidurans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
NAD+ | mutant R301A, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.97 | - |
NAD+ | mutant R301K, pH 7.3, 25°C | Pseudomonas stutzeri | |
1.03 | - |
NAD+ | mutant 17X-PTDH, pH 7.3, 25°C | Pseudomonas stutzeri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00211 | - |
phosphonate | mutant R301A, pH 7.3, 25°C | Pseudomonas stutzeri | |
0.901 | - |
phosphonate | mutant W134A, pH 7.3, 25°C | Pseudomonas stutzeri | |
4.05 | - |
phosphonate | mutant R301K, pH 7.3, 25°C | Pseudomonas stutzeri | |
12.4 | - |
phosphonate | mutant W134F, pH 7.3, 25°C | Pseudomonas stutzeri | |
24.6 | - |
phosphonate | mutant S295A, pH 7.3, 25°C | Pseudomonas stutzeri | |
94.4 | - |
phosphonate | mutant Y139F, pH 7.3, 25°C | Pseudomonas stutzeri | |
117 | - |
phosphonate | mutant 17X-PTDH, pH 7.3, 25°C | Pseudomonas stutzeri |