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Literature summary for 1.20.1.1 extracted from
- Purification and characterization of a novel phosphorus-oxidizing enzyme from Pseudomonas stutzeri WM88 (2001), J. Biol. Chem., 276, 17429-17436.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in Escherichia coli | Pseudomonas stutzeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | - |
Pseudomonas stutzeri |  |
| D,L-hydroxyisocaproate | - |
Pseudomonas stutzeri | |
| D-2-hydroxy-4-methylvalerate | - |
Pseudomonas stutzeri | |
| D-glycerate | - |
Pseudomonas stutzeri | |
| formate | - |
Pseudomonas stutzeri | |
| NaCl | - |
Pseudomonas stutzeri | |
| NADH | competitive | Pseudomonas stutzeri | |
| nitrite | - |
Pseudomonas stutzeri | |
| sulfite | dead end inhibitor, competitive | Pseudomonas stutzeri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.053 | - |
phosphite | - |
Pseudomonas stutzeri | |
| 0.055 | - |
NAD+ | - |
Pseudomonas stutzeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
gel filtration | Pseudomonas stutzeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphite + H2O + NAD+ | Pseudomonas stutzeri | use of phosphite as energy source, regeneration of NADH | phosphate + NADH + H+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | O69054 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Pseudomonas stutzeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.2 | - |
- |
Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxypyruvate + NAD+ | very poor substrate | Pseudomonas stutzeri | pyruvate + NADH | - |
? | |
| phosphite + H2O + NAD+ | - |
Pseudomonas stutzeri | phosphate + NADH + H+ | - |
ir | |
| phosphite + H2O + NAD+ | use of phosphite as energy source, regeneration of NADH | Pseudomonas stutzeri | phosphate + NADH + H+ | - |
ir | |
| phosphite + H2O + NADP+ | NADP poorly substitutes for NAD+ | Pseudomonas stutzeri | phosphate + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | gel filtration | Pseudomonas stutzeri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.33 | - |
phosphite | - |
Pseudomonas stutzeri | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas stutzeri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10 | low activity | Pseudomonas stutzeri |
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