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Literature summary for 1.2.99.7 extracted from

  • Romao, M.J.; Barata, B.A.; Archer, M.; Lobeck, K.; Moura, I.; Carrondo, M.A.; LeGall, J.; Lottspeich, F.; Huber, R.; Moura, J.J.
    Subunit composition, crystallization and preliminary crystallographic studies of the Desulfovibrio gigas aldehyde oxidoreductase containing molybdenum and [2Fe-2S] centers (1993), Eur. J. Biochem., 215, 729-732.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
vapour-diffusion using sitting drops and a reservoir containing 100 mM Hepes, pH 7.5, 200 mM MgCl2 and 30% isopropanol, droplets are prepared by mixing 0.004 ml of a 13 mg/ml protein solution in 10 mM Tris, pH 7.6 with 0.002 ml reservoir solution, single crystals are obtained within 3-6 weeks at 4°C, crystals diffract to 3.0 A Megalodesulfovibrio gigas

Metals/Ions

Metals/Ions Comment Organism Structure
Iron [Fe-2S] cluster Megalodesulfovibrio gigas
Molybdenum bound to a pterin cofactor and [2Fe-2S] cluster Megalodesulfovibrio gigas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
2 * 100000, SDS-PAGE Megalodesulfovibrio gigas
200000
-
gel filtration Megalodesulfovibrio gigas

Organism

Organism UniProt Comment Textmining
Megalodesulfovibrio gigas
-
NCIB 9332
-

Purification (Commentary)

Purification (Comment) Organism
-
Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + H2O + electron acceptor
-
Megalodesulfovibrio gigas acetate + reduced electron acceptor
-
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Subunits

Subunits Comment Organism
dimer 2 * 100000, SDS-PAGE Megalodesulfovibrio gigas