Crystallization (Comment) | Organism |
---|---|
vapour-diffusion using sitting drops and a reservoir containing 100 mM Hepes, pH 7.5, 200 mM MgCl2 and 30% isopropanol, droplets are prepared by mixing 0.004 ml of a 13 mg/ml protein solution in 10 mM Tris, pH 7.6 with 0.002 ml reservoir solution, single crystals are obtained within 3-6 weeks at 4°C, crystals diffract to 3.0 A | Megalodesulfovibrio gigas |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | [Fe-2S] cluster | Megalodesulfovibrio gigas | |
Molybdenum | bound to a pterin cofactor and [2Fe-2S] cluster | Megalodesulfovibrio gigas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
2 * 100000, SDS-PAGE | Megalodesulfovibrio gigas |
200000 | - |
gel filtration | Megalodesulfovibrio gigas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Megalodesulfovibrio gigas | - |
NCIB 9332 | - |
Purification (Comment) | Organism |
---|---|
- |
Megalodesulfovibrio gigas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + H2O + electron acceptor | - |
Megalodesulfovibrio gigas | acetate + reduced electron acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 100000, SDS-PAGE | Megalodesulfovibrio gigas |