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Literature summary for 1.2.99.7 extracted from

  • Barata, B.A.; LeGall, J.; Moura, J.J.
    Aldehyde oxidoreductase activity in Desulfovibrio gigas: in vitro reconstitution of an electron-transfer chain from aldehydes to the production of molecular hydrogen (1993), Biochemistry, 32, 11559-11568.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
 Salicylaldehyde 25°C, pH 7.6 Megalodesulfovibrio gigas
0.013
-
 acetaldehyde 25°C, pH 7.6 Megalodesulfovibrio gigas
0.015
-
 propionaldehyde 25°C, pH 7.6 Megalodesulfovibrio gigas
0.052
-
 benzaldehyde 25°C, pH 7.6 Megalodesulfovibrio gigas

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains [2Fe-2S] cluster Megalodesulfovibrio gigas
Molybdenum molybdenum reductase Megalodesulfovibrio gigas

Organism

Organism UniProt Comment Textmining
Megalodesulfovibrio gigas
-
 NCIB 9332
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-52, DEAE-Biogel, hydroxylapatite, HPLC Megalodesulfovibrio gigas

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17
-
-
 Megalodesulfovibrio gigas

Storage Stability

Storage Stability Organism
-30°C, phosphate buffer, no loss of activity after thawing Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-pyridinecarboxaldehyde + H2O + 2,6-dichlorophenol-indophenol 159% of activity with acetaldehyde Megalodesulfovibrio gigas 3-pyridinecarboxylate + reduced 2,6-dichlorophenol-indophenol
-
 ?
acetaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
 Megalodesulfovibrio gigas acetate + reduced 2,6-dichlorophenol-indophenol
-
 ?
benzaldehyde + 2 ferricyanide + H2O 156% of activity with 2,6-dichlorophenol-indophenol Megalodesulfovibrio gigas benzoate + 2 ferrocyanide + 2 H+
-
 ?
benzaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
 Megalodesulfovibrio gigas benzoate + reduced 2,6-dichlorophenol-indophenol
-
 ?
benzaldehyde + H2O + cytochrome c from horse heart, 25% of activity with 2,6-dichlorophenol-indophenol Megalodesulfovibrio gigas benzoate + reduced cytochrome c
-
 ?
benzaldehyde + H2O + cytochrome c3 from, Desulfovibrio gigas, 20% of activity with 2,6-dichlorophenol-indophenol Megalodesulfovibrio gigas benzoate + reduced cytochrome c3
-
 ?
benzaldehyde + H2O + methylene blue 156% of activity with 2,6-dichlorophenol-indophenol Megalodesulfovibrio gigas benzoate + reduced methylene blue
-
 ?
furfuraldehyde + H2O + 2,6-dichlorophenol-indophenol 27% of activity with acetaldehyde Megalodesulfovibrio gigas furfurate + reduced 2,6-dichlorophenol-indophenol
-
 ?
propionaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
 Megalodesulfovibrio gigas propionate + reduced 2,6-dichlorophenol-indophenol
-
 ?
salicylaldehyde + H2O + 2,6-dichlorophenol-indophenol
-
 Megalodesulfovibrio gigas salicylate + reduced 2,6-dichlorophenol-indophenol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 Salicylaldehyde 25°C, pH 7.6 Megalodesulfovibrio gigas
0.44
-
 propionaldehyde 25°C, pH 7.6 Megalodesulfovibrio gigas
1.14
-
 acetaldehyde 25°C, pH 7.6 Megalodesulfovibrio gigas
1.43
-
 benzaldehyde 25°C, pH 7.6 Megalodesulfovibrio gigas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Megalodesulfovibrio gigas