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Literature summary for 1.2.99.6 extracted from

  • Rauh, D.; Graentzdoerffer, A.; Granderath, K.; Andreesen, J.R.; Pich, A.
    Tungsten-containing aldehyde oxidoreductase of Eubacterium acidaminophilum. Isolation, characterization and molecular analysis (2004), Eur. J. Biochem., 271, 212-219.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0073
-
 benzaldehyde
-
 Peptoclostridium acidaminophilum
0.012
-
 Butyraldehyde
-
 Peptoclostridium acidaminophilum
0.016
-
 DL-glyceraldehyde
-
 Peptoclostridium acidaminophilum
0.017
-
 propionaldehyde
-
 Peptoclostridium acidaminophilum
0.019
-
 acetaldehyde
-
 Peptoclostridium acidaminophilum
0.033
-
 Isovaleraldehyde
-
 Peptoclostridium acidaminophilum
0.067
-
 phenylacetaldehyde
-
 Peptoclostridium acidaminophilum
0.084
-
 Glutaraldehyde
-
 Peptoclostridium acidaminophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 gel filtration Peptoclostridium acidaminophilum
67000
-
 1 * 67000, SDS-PAGE Peptoclostridium acidaminophilum

Organism

Organism UniProt Comment Textmining
Peptoclostridium acidaminophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using strict anaerobic conditions Peptoclostridium acidaminophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.6
-
 34°C, pH 8.5 Peptoclostridium acidaminophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + benzyl viologen
-
 Peptoclostridium acidaminophilum acetic acid + reduced benzyl viologen
-
 ?
benzaldehyde + benzyl viologen 96% of activity compared to acetaldehyde Peptoclostridium acidaminophilum benzoic acid + reduced benzyl viologen
-
 ?
Butyraldehyde + benzyl viologen 101% of activity compared to acetaldehyde Peptoclostridium acidaminophilum Butanoate + reduced benzyl viologen
-
 ?
crotonaldehyde + benzyl viologen 47% of activity compared to acetaldehyde Peptoclostridium acidaminophilum crotonic acid + reduced benzyl viologen
-
 ?
DL-glyceraldehyde + benzyl viologen 9% of activity compared to acetaldehyde Peptoclostridium acidaminophilum alpha,beta-dihydroxypropionic acid + reduced benzyl viologen
-
 ?
formaldehyde + benzyl viologen 2% of activity compared to acetaldehyde Peptoclostridium acidaminophilum formic acid + reduced benzyl viologen
-
 ?
glutaraldehyde + benzyl viologen 19% of activity compared to acetaldehyde Peptoclostridium acidaminophilum glutaric acid + reduced benzyl viologen
-
 ?
glyoxylate + benzyl viologen 6% of activity compared to acetaldehyde Peptoclostridium acidaminophilum oxalic acid + reduced benzyl viologen
-
 ?
isovaleraldehyde + benzyl viologen 66% of activity compared to acetaldehyde Peptoclostridium acidaminophilum isopentanoic acid + reduced benzyl viologen
-
 ?
phenylacetaldehyde + benzyl viologen 20% of activity compared to acetaldehyde Peptoclostridium acidaminophilum phenylacetic acid + reduced benzyl viologen
-
 ?
propionaldehyde + benzyl viologen 119% of activity compared to acetaldehyde Peptoclostridium acidaminophilum propionic acid + reduced benzyl viologen
-
 ?
salicylaldehyde + benzyl viologen 4% of activity compared to acetaldehyde Peptoclostridium acidaminophilum salicylic acid + reduced benzyl viologen
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 67000, SDS-PAGE Peptoclostridium acidaminophilum