BRENDA - Enzyme Database
show all sequences of 1.2.99.6

Purification and some properties of the tungsten-containing carboxylic acid reductase from Clostridium formicoaceticum

White, H.; Feicht, R.; Huber, C.; Lottspeich, F.; Simon, H.; Biol. Chem. Hoppe-Seyler 372, 999-1005 (1991)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
Butanoate
-
Clostridium formicaceticum
1.2
-
4-Chlorobenzoate
-
Clostridium formicaceticum
1.4
-
4-Fluorobenzoate
-
Clostridium formicaceticum
2.1
-
4-Methoxybenzoate
-
Clostridium formicaceticum
3.8
-
3-chlorobenzoate
-
Clostridium formicaceticum
5
-
4-Methylbenzoate
-
Clostridium formicaceticum
6.3
-
propanoate
3-fluorobenzoate
Clostridium formicaceticum
6.4
-
propanoate
-
Clostridium formicaceticum
12.8
-
3-methylbenzoate
-
Clostridium formicaceticum
16.7
-
benzoate
-
Clostridium formicaceticum
44.9
-
3-Methoxybenzoate
-
Clostridium formicaceticum
50.4
-
3-hydroxybenzoate
-
Clostridium formicaceticum
382
-
4-hydroxybenzoate
-
Clostridium formicaceticum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
contains 11 mol of iron per mol of enzyme
Clostridium formicaceticum
Tungsten
contains 1.4 mol of tungsten per mol of enzyme
Clostridium formicaceticum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
2 * 67000, SDS-PAGE
Clostridium formicaceticum
125000
-
gel filtration
Clostridium formicaceticum
Organism
Organism
UniProt
Commentary
Textmining
Clostridium formicaceticum
-
-
-
Oxidation Stability
Oxidation Stability
Organism
22% loss of activity after 5 min in air, at 4C
Clostridium formicaceticum
Purification (Commentary)
Purification (Commentary)
Organism
-
Clostridium formicaceticum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Clostridium formicaceticum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Methoxybenzaldhyde + carbamoyl methyl viologen
-
-
-
?
3-Methylbenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Methoxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Methylbenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
Benzaldehyde + carbamoyl methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Benzoate + reduced carbamoyl methyl viologen
-
-
-
?
Butanal + NH2CO-methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Butanoate + reduced NH2CO-methyl viologen
-
-
-
?
Propanal + benzyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced benzyl viologen
-
11943
Clostridium formicaceticum
?
Propanal + methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced methyl viologen
-
11943
Clostridium formicaceticum
?
Propanal + NH2CO-methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
?
Propanal + reduced 1,1',2,2'-tetramethylviologen
-
11943
Clostridium formicaceticum
Propanoate + 1,1',2,2'-tetramethylviologen
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 67000, SDS-PAGE
Clostridium formicaceticum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
aldehyde dehydrogenation
Clostridium formicaceticum
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
50
60% of maximal activity at 30C and 50C, inactive at 60C, aldehyde dehydrogenation
Clostridium formicaceticum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of propanoate or benzoate
Clostridium formicaceticum
9
-
butanal and NH2-CO-methyl viologen as substrates
Clostridium formicaceticum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
7
pH 6.0: optimum, pH 7.0: 25% of maximal activity, reduction of propanoate or benzoate
Clostridium formicaceticum
8
10
about 90% of maximal activity, butanal and NH2-CO-methyl viologen as substrates
Clostridium formicaceticum
Cofactor
Cofactor
Commentary
Organism
Structure
pterin
contains 1.6 mol of pterin-6-carboxylic acid per mol of enzyme
Clostridium formicaceticum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pterin
contains 1.6 mol of pterin-6-carboxylic acid per mol of enzyme
Clostridium formicaceticum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
Butanoate
-
Clostridium formicaceticum
1.2
-
4-Chlorobenzoate
-
Clostridium formicaceticum
1.4
-
4-Fluorobenzoate
-
Clostridium formicaceticum
2.1
-
4-Methoxybenzoate
-
Clostridium formicaceticum
3.8
-
3-chlorobenzoate
-
Clostridium formicaceticum
5
-
4-Methylbenzoate
-
Clostridium formicaceticum
6.3
-
propanoate
3-fluorobenzoate
Clostridium formicaceticum
6.4
-
propanoate
-
Clostridium formicaceticum
12.8
-
3-methylbenzoate
-
Clostridium formicaceticum
16.7
-
benzoate
-
Clostridium formicaceticum
44.9
-
3-Methoxybenzoate
-
Clostridium formicaceticum
50.4
-
3-hydroxybenzoate
-
Clostridium formicaceticum
382
-
4-hydroxybenzoate
-
Clostridium formicaceticum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
contains 11 mol of iron per mol of enzyme
Clostridium formicaceticum
Tungsten
contains 1.4 mol of tungsten per mol of enzyme
Clostridium formicaceticum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
2 * 67000, SDS-PAGE
Clostridium formicaceticum
125000
-
gel filtration
Clostridium formicaceticum
Oxidation Stability (protein specific)
Oxidation Stability
Organism
22% loss of activity after 5 min in air, at 4C
Clostridium formicaceticum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium formicaceticum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Clostridium formicaceticum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Methoxybenzaldhyde + carbamoyl methyl viologen
-
-
-
?
3-Methylbenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Methoxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Methylbenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
Benzaldehyde + carbamoyl methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Benzoate + reduced carbamoyl methyl viologen
-
-
-
?
Butanal + NH2CO-methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Butanoate + reduced NH2CO-methyl viologen
-
-
-
?
Propanal + benzyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced benzyl viologen
-
11943
Clostridium formicaceticum
?
Propanal + methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced methyl viologen
-
11943
Clostridium formicaceticum
?
Propanal + NH2CO-methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
?
Propanal + reduced 1,1',2,2'-tetramethylviologen
-
11943
Clostridium formicaceticum
Propanoate + 1,1',2,2'-tetramethylviologen
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 67000, SDS-PAGE
Clostridium formicaceticum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
aldehyde dehydrogenation
Clostridium formicaceticum
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
50
60% of maximal activity at 30C and 50C, inactive at 60C, aldehyde dehydrogenation
Clostridium formicaceticum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of propanoate or benzoate
Clostridium formicaceticum
9
-
butanal and NH2-CO-methyl viologen as substrates
Clostridium formicaceticum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
7
pH 6.0: optimum, pH 7.0: 25% of maximal activity, reduction of propanoate or benzoate
Clostridium formicaceticum
8
10
about 90% of maximal activity, butanal and NH2-CO-methyl viologen as substrates
Clostridium formicaceticum
Other publictions for EC 1.2.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
655482
Rauh
Tungsten-containing aldehyde o ...
Peptoclostridium acidaminophilum
Eur. J. Biochem.
271
212-219
2004
-
-
-
-
-
-
-
8
-
-
2
-
-
7
-
-
1
-
-
-
1
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
2
-
-
-
-
1
-
-
1
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654439
Andrade
Kinetic behavior of Desulfovib ...
Desulfovibrio gigas
Biochem. Biophys. Res. Commun.
308
73-78
2003
-
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
3
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
11935
Heider
Purification, characterization ...
Thermococcus sp., Thermococcus sp. ES-1
J. Bacteriol.
177
4757-4764
1995
-
-
-
-
-
-
9
15
-
6
2
3
-
3
1
-
1
-
-
1
1
-
23
1
-
1
-
2
15
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
9
-
15
-
6
2
3
-
1
-
1
-
1
1
-
23
1
1
-
2
15
1
-
-
-
-
-
-
-
-
-
11936
Hensgens
Purification and characterizat ...
Desulfovibrio gigas, Desulfovibrio gigas NCIMB 9332
J. Bacteriol.
177
6195-6200
1995
-
-
-
-
-
-
4
5
-
4
2
-
-
10
1
-
1
-
-
-
1
-
21
1
-
1
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
5
-
4
2
-
-
1
-
1
-
-
1
-
21
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
11939
Huber
-
Pterin cofactor, substrate spe ...
Moorella thermoacetica
Arch. Microbiol.
164
110-118
1995
-
-
-
-
-
-
1
34
-
-
-
-
-
1
-
-
-
-
-
-
-
-
25
-
-
2
1
-
-
5
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
34
-
-
-
-
-
-
-
-
-
-
-
-
25
-
2
1
-
-
5
4
-
-
-
-
-
-
-
-
11940
Huber
-
Further characterization of tw ...
Clostridium formicaceticum
Arch. Microbiol.
162
303-309
1994
-
-
-
-
-
-
3
5
-
-
-
-
-
1
1
-
-
1
-
-
-
-
4
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
5
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
11941
White
The role of tungstate and/or m ...
Moorella thermoacetica, no activity in Butyribacterium methylotrophicum, no activity in Clostridium aceticum, no activity in Eubacterium limosum
Arch. Microbiol.
158
81-84
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11942
Strobl
The tungsten-containing aldehy ...
Moorella thermoacetica
Biol. Chem. Hoppe-Seyler
373
123-132
1992
-
-
-
-
-
-
-
-
-
4
7
-
-
3
-
-
1
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
7
-
-
-
-
1
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11938
Mukund
The novel tungsten-iron-sulfur ...
Pyrococcus furiosus
J. Biol. Chem.
266
14208-14216
1991
-
-
-
-
-
-
1
1
-
2
-
1
-
9
1
-
1
-
-
-
1
-
6
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
1
-
1
-
1
-
-
1
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
11943
White
Purification and some properti ...
Clostridium formicaceticum
Biol. Chem. Hoppe-Seyler
372
999-1005
1991
-
-
-
-
-
-
-
13
-
2
2
-
-
6
1
-
1
-
-
-
1
-
16
1
-
1
1
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
13
-
2
2
-
-
1
-
1
-
-
1
-
16
1
1
1
-
-
2
2
-
-
-
-
-
-
-
-
11937
White
Carboxylic acid reductase: a n ...
Moorella thermoacetica
Eur. J. Biochem.
184
89-96
1989
-
-
-
-
-
-
-
-
-
1
1
-
-
2
1
-
1
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