BRENDA - Enzyme Database
show all sequences of 1.2.99.6

Pterin cofactor, substrate specificity, and observations on the kinetics of the reversible tungsten-containing aldehyde oxidoreductase from Clostridium thermoaceticum

Huber, C.; Skopan, H.; Feicht, R.; White, H.; Simon, H.; Arch. Microbiol. 164, 110-118 (1995)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-Hydroxypyridine-5-carboxylate
-
Moorella thermoacetica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
4-methoxybenzaldehyde
terephthalaldehyde, with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.005
-
4-methoxybenzaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.006
-
Butanal
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.008
-
propanal
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.009
-
benzaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.01
-
ES-1 ferredoxin
acetaldehyde, with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.012
-
cinnamaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.02
-
benzaldehyde
3,4-dimethoxy-5-hydroxybenzaldehyde, with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.03
-
reduced carbamoyl methyl viologen
with propanoate as cosubstrate
Moorella thermoacetica
0.05
-
Indoleacetaldehyde
reduced carbamoyl methyl viologen, with benzoate as cosubstrate
Moorella thermoacetica
0.1
-
5-Phenyl-2,4-pentadienate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.16
-
reduced carbamoyl methyl viologen
with benzoate as cosubstrate
Moorella thermoacetica
1
-
4-Chlorobenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
1.6
-
methanal
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
1.6
-
methanal
4-fluorobenzoate, with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
1.6
-
methanal
4-methoxybenzoate, with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
1.7
-
cinnamate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
2.4
-
3-chlorobenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
2.9
-
methyl viologen
3-methoxybenzoate, with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
3
-
4-hydroxy-3-methoxybenzaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
3
-
4-hydroxy-3-methoxybenzaldehyde
4-methylbenzoate, with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
3.5
-
3-Fluorobenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
4
-
terephthalate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
5.6
-
acetate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
6.1
-
3-methylbenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
6.3
-
propanoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
8
-
4-(hydroxymethyl)benzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
10
-
3,5-dimethoxy-4-hydroxybenzaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
12
-
4-hydroxybenzaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
31
-
benzoate
with reduced carbamoyl methyl viologen as cosubstrate, pH 4.5
Moorella thermoacetica
53
-
benzoate
with reduced carbamoyl methyl viologen as cosubstrate, pH 5.5
Moorella thermoacetica
78
-
3-hydroxybenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
78
-
3-hydroxybenzoate
pyridine-4-carboxylate, with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
130
-
4-hydroxybenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
Organism
Organism
UniProt
Commentary
Textmining
Moorella thermoacetica
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3,4-Dimethoxy-5-hydroxy-benzaldehyde + carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3,4-Dimethoxy-5-hydroxy-benzoate + reduced carbamoyl methyl viologen
-
-
-
?
3,5-Dimethoxy-4-hydroxy-benzaldehyde + carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3,5-Dimethoxy-4-hydroxy-benzoate + reduced carbamoyl methyl viologen
-
-
-
?
3-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3-Methoxybenzaldhyde + carbamoyl methyl viologen
-
-
-
?
3-Methylbenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-(Hydroxymethyl)benzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
4-(Hydroxymethyl)benzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
4-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
4-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Hydroxy-3-methoxy-benzaldehyde + carbamoyl methyl viologen
-
11939
Moorella thermoacetica
4-Hydroxy-3-methoxy-benzoate + reduced carbamoyl methyl viologen
-
-
-
?
4-Hydroxybenzaldehyde + carbamoyl methyl viologen
r
11939
Moorella thermoacetica
4-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
4-Methoxybenzaldehyde + benzyl viologen
-
11939
Moorella thermoacetica
4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
4-Methylbenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
4-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
5-Phenyl-2,4-pentadienate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
? + carbamoyl methyl viologen
-
-
-
?
Acetaldehyde + carbamoyl methyl viologen
r
11939
Moorella thermoacetica
acetate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
Benzaldehyde + carbamoyl methyl viologen
r
11939
Moorella thermoacetica
Benzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
Butanal + NH2CO-methyl viologen
-
11939
Moorella thermoacetica
Butanoate + reduced NH2CO-methyl viologen
-
-
-
?
Cinnamaldehyde + carbamoyl methyl viologen
r
11939
Moorella thermoacetica
Cinnamate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
Indoleacetaldehyde + benzyl viologen
-
11939
Moorella thermoacetica
Indoleacetate + reduced benzyl viologen
-
-
-
?
Methanal + carbamoyl methyl viologen
-
11939
Moorella thermoacetica
Formate + reduced methyl viologen
-
-
-
?
Propanal + methyl viologen
-
11939
Moorella thermoacetica
Propanoate + reduced methyl viologen
-
-
-
?
Propanal + NH2CO-methyl viologen
r
11939
Moorella thermoacetica
Propanoate + reduced carbamoyl methyl viologen
-
-
-
?
Pyridine 4-carboxylate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
Pyridine 4-carboxaldehyde + carbamoyl methyl viologen
-
-
-
?
terephthalaldehyde + carbamoyl methyl viologen
r
11939
Moorella thermoacetica
terephthalate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
46
-
at pH 4.5
Moorella thermoacetica
51
-
propanoate as substrate, pH 4.8
Moorella thermoacetica
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
51
71
51°C: optimum, 71°C: about 60% of maximal activity, propanoate and reduced carbamoyl methyl viologen as substrates
Moorella thermoacetica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1
-
4-fluorobenzoate
Moorella thermoacetica
4.4
4.7
benzoate and reduced carbamoyl methyl viologen as substrate
Moorella thermoacetica
4.7
-
propanoate and reduced carbamoyl methyl viologen as substrate
Moorella thermoacetica
4.7
-
4-methoxybenzoate
Moorella thermoacetica
4.7
-
4-methoxybenzoate and reduced carbamoyl methyl viologen as substrate
Moorella thermoacetica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.9
4.1
pH 3.9: 80% of maximal activity, pH 4.1: optimum, 4-fluorobenzoate and reduced carbamoyl methyl viologen as substrates
Moorella thermoacetica
3.9
4.7
pH 3.9: 55% of maximal activity, pH 4.7: optimum, 4-methoxybenzoate and reduced carbamoyl methyl viologen as substrates
Moorella thermoacetica
3.9
4.7
pH 3.9: 55% of maximal activity, pH 4.3-4.7: optimum
Moorella thermoacetica
4.7
5.5
pH 4.7: optimum, pH 5.5: 20% of maximal activity, propanoate reduced carbamoyl methyl viologen as substrates
Moorella thermoacetica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-Hydroxypyridine-5-carboxylate
-
Moorella thermoacetica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
4-methoxybenzaldehyde
terephthalaldehyde, with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.005
-
4-methoxybenzaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.006
-
Butanal
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.008
-
propanal
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.009
-
benzaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.01
-
ES-1 ferredoxin
acetaldehyde, with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.012
-
cinnamaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.02
-
benzaldehyde
3,4-dimethoxy-5-hydroxybenzaldehyde, with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.03
-
reduced carbamoyl methyl viologen
with propanoate as cosubstrate
Moorella thermoacetica
0.05
-
Indoleacetaldehyde
reduced carbamoyl methyl viologen, with benzoate as cosubstrate
Moorella thermoacetica
0.1
-
5-Phenyl-2,4-pentadienate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
0.16
-
reduced carbamoyl methyl viologen
with benzoate as cosubstrate
Moorella thermoacetica
1
-
4-Chlorobenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
1.6
-
methanal
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
1.6
-
methanal
4-fluorobenzoate, with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
1.6
-
methanal
4-methoxybenzoate, with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
1.7
-
cinnamate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
2.4
-
3-chlorobenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
2.9
-
methyl viologen
3-methoxybenzoate, with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
3
-
4-hydroxy-3-methoxybenzaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
3
-
4-hydroxy-3-methoxybenzaldehyde
4-methylbenzoate, with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
3.5
-
3-Fluorobenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
4
-
terephthalate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
5.6
-
acetate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
6.1
-
3-methylbenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
6.3
-
propanoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
8
-
4-(hydroxymethyl)benzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
10
-
3,5-dimethoxy-4-hydroxybenzaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
12
-
4-hydroxybenzaldehyde
with carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
31
-
benzoate
with reduced carbamoyl methyl viologen as cosubstrate, pH 4.5
Moorella thermoacetica
53
-
benzoate
with reduced carbamoyl methyl viologen as cosubstrate, pH 5.5
Moorella thermoacetica
78
-
3-hydroxybenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
78
-
3-hydroxybenzoate
pyridine-4-carboxylate, with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
130
-
4-hydroxybenzoate
with reduced carbamoyl methyl viologen as cosubstrate
Moorella thermoacetica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3,4-Dimethoxy-5-hydroxy-benzaldehyde + carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3,4-Dimethoxy-5-hydroxy-benzoate + reduced carbamoyl methyl viologen
-
-
-
?
3,5-Dimethoxy-4-hydroxy-benzaldehyde + carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3,5-Dimethoxy-4-hydroxy-benzoate + reduced carbamoyl methyl viologen
-
-
-
?
3-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
3-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3-Methoxybenzaldhyde + carbamoyl methyl viologen
-
-
-
?
3-Methylbenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
3-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-(Hydroxymethyl)benzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
4-(Hydroxymethyl)benzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
4-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
4-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
4-Hydroxy-3-methoxy-benzaldehyde + carbamoyl methyl viologen
-
11939
Moorella thermoacetica
4-Hydroxy-3-methoxy-benzoate + reduced carbamoyl methyl viologen
-
-
-
?
4-Hydroxybenzaldehyde + carbamoyl methyl viologen
r
11939
Moorella thermoacetica
4-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
4-Methoxybenzaldehyde + benzyl viologen
-
11939
Moorella thermoacetica
4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
4-Methylbenzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
4-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
5-Phenyl-2,4-pentadienate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
? + carbamoyl methyl viologen
-
-
-
?
Acetaldehyde + carbamoyl methyl viologen
r
11939
Moorella thermoacetica
acetate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
Benzaldehyde + carbamoyl methyl viologen
r
11939
Moorella thermoacetica
Benzoate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
Butanal + NH2CO-methyl viologen
-
11939
Moorella thermoacetica
Butanoate + reduced NH2CO-methyl viologen
-
-
-
?
Cinnamaldehyde + carbamoyl methyl viologen
r
11939
Moorella thermoacetica
Cinnamate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
Indoleacetaldehyde + benzyl viologen
-
11939
Moorella thermoacetica
Indoleacetate + reduced benzyl viologen
-
-
-
?
Methanal + carbamoyl methyl viologen
-
11939
Moorella thermoacetica
Formate + reduced methyl viologen
-
-
-
?
Propanal + methyl viologen
-
11939
Moorella thermoacetica
Propanoate + reduced methyl viologen
-
-
-
?
Propanal + NH2CO-methyl viologen
r
11939
Moorella thermoacetica
Propanoate + reduced carbamoyl methyl viologen
-
-
-
?
Pyridine 4-carboxylate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
Pyridine 4-carboxaldehyde + carbamoyl methyl viologen
-
-
-
?
terephthalaldehyde + carbamoyl methyl viologen
r
11939
Moorella thermoacetica
terephthalate + reduced carbamoyl methyl viologen
-
11939
Moorella thermoacetica
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
46
-
at pH 4.5
Moorella thermoacetica
51
-
propanoate as substrate, pH 4.8
Moorella thermoacetica
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
51
71
51°C: optimum, 71°C: about 60% of maximal activity, propanoate and reduced carbamoyl methyl viologen as substrates
Moorella thermoacetica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1
-
4-fluorobenzoate
Moorella thermoacetica
4.4
4.7
benzoate and reduced carbamoyl methyl viologen as substrate
Moorella thermoacetica
4.7
-
propanoate and reduced carbamoyl methyl viologen as substrate
Moorella thermoacetica
4.7
-
4-methoxybenzoate
Moorella thermoacetica
4.7
-
4-methoxybenzoate and reduced carbamoyl methyl viologen as substrate
Moorella thermoacetica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.9
4.1
pH 3.9: 80% of maximal activity, pH 4.1: optimum, 4-fluorobenzoate and reduced carbamoyl methyl viologen as substrates
Moorella thermoacetica
3.9
4.7
pH 3.9: 55% of maximal activity, pH 4.7: optimum, 4-methoxybenzoate and reduced carbamoyl methyl viologen as substrates
Moorella thermoacetica
3.9
4.7
pH 3.9: 55% of maximal activity, pH 4.3-4.7: optimum
Moorella thermoacetica
4.7
5.5
pH 4.7: optimum, pH 5.5: 20% of maximal activity, propanoate reduced carbamoyl methyl viologen as substrates
Moorella thermoacetica
Other publictions for EC 1.2.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
655482
Rauh
Tungsten-containing aldehyde o ...
Peptoclostridium acidaminophilum
Eur. J. Biochem.
271
212-219
2004
-
-
-
-
-
-
-
8
-
-
2
-
-
7
-
-
1
-
-
-
1
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
2
-
-
-
-
1
-
-
1
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654439
Andrade
Kinetic behavior of Desulfovib ...
Desulfovibrio gigas
Biochem. Biophys. Res. Commun.
308
73-78
2003
-
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
3
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
11935
Heider
Purification, characterization ...
Thermococcus sp., Thermococcus sp. ES-1
J. Bacteriol.
177
4757-4764
1995
-
-
-
-
-
-
9
15
-
6
2
3
-
3
1
-
1
-
-
1
1
-
23
1
-
1
-
2
15
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
9
-
15
-
6
2
3
-
1
-
1
-
1
1
-
23
1
1
-
2
15
1
-
-
-
-
-
-
-
-
-
11936
Hensgens
Purification and characterizat ...
Desulfovibrio gigas, Desulfovibrio gigas NCIMB 9332
J. Bacteriol.
177
6195-6200
1995
-
-
-
-
-
-
4
5
-
4
2
-
-
10
1
-
1
-
-
-
1
-
21
1
-
1
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
5
-
4
2
-
-
1
-
1
-
-
1
-
21
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
11939
Huber
-
Pterin cofactor, substrate spe ...
Moorella thermoacetica
Arch. Microbiol.
164
110-118
1995
-
-
-
-
-
-
1
34
-
-
-
-
-
1
-
-
-
-
-
-
-
-
25
-
-
2
1
-
-
5
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
34
-
-
-
-
-
-
-
-
-
-
-
-
25
-
2
1
-
-
5
4
-
-
-
-
-
-
-
-
11940
Huber
-
Further characterization of tw ...
Clostridium formicaceticum
Arch. Microbiol.
162
303-309
1994
-
-
-
-
-
-
3
5
-
-
-
-
-
1
1
-
-
1
-
-
-
-
4
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
5
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
11941
White
The role of tungstate and/or m ...
Moorella thermoacetica, no activity in Butyribacterium methylotrophicum, no activity in Clostridium aceticum, no activity in Eubacterium limosum
Arch. Microbiol.
158
81-84
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11942
Strobl
The tungsten-containing aldehy ...
Moorella thermoacetica
Biol. Chem. Hoppe-Seyler
373
123-132
1992
-
-
-
-
-
-
-
-
-
4
7
-
-
3
-
-
1
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
7
-
-
-
-
1
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11938
Mukund
The novel tungsten-iron-sulfur ...
Pyrococcus furiosus
J. Biol. Chem.
266
14208-14216
1991
-
-
-
-
-
-
1
1
-
2
-
1
-
9
1
-
1
-
-
-
1
-
6
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
1
-
1
-
1
-
-
1
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
11943
White
Purification and some properti ...
Clostridium formicaceticum
Biol. Chem. Hoppe-Seyler
372
999-1005
1991
-
-
-
-
-
-
-
13
-
2
2
-
-
6
1
-
1
-
-
-
1
-
16
1
-
1
1
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
13
-
2
2
-
-
1
-
1
-
-
1
-
16
1
1
1
-
-
2
2
-
-
-
-
-
-
-
-
11937
White
Carboxylic acid reductase: a n ...
Moorella thermoacetica
Eur. J. Biochem.
184
89-96
1989
-
-
-
-
-
-
-
-
-
1
1
-
-
2
1
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-