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Literature summary for 1.2.99.6 extracted from

  • Hensgens, C.M.H.; Hagen, W.R.; Hansen, T.A.
    Purification and characterization of a benzylviologen-linked tungsten-containing aldehyde oxidoreductase from Desulfovibrio gigas (1995), J. Bacteriol., 177, 6195-6200.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.99.6

Inhibitors

Inhibitors Comment Organism Structure
AsO2-
-
 Megalodesulfovibrio gigas
CN-
-
 Megalodesulfovibrio gigas
iodoacetate
-
 Megalodesulfovibrio gigas
methanol
-
 Megalodesulfovibrio gigas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0108
-
 propanal with benzyl viologen as cosubstrate Megalodesulfovibrio gigas
0.0125
-
 acetaldehyde with benzyl viologen as cosubstrate Megalodesulfovibrio gigas
0.02
-
 benzaldehyde with benzyl viologen as cosubstrate Megalodesulfovibrio gigas
0.0308
-
 furfural with benzyl viologen as cosubstrate Megalodesulfovibrio gigas
0.55
-
 benzyl viologen
-
 Megalodesulfovibrio gigas

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains 4.8 gatom of Fe per mol of subunit Megalodesulfovibrio gigas
Iron possibly contains a [4Fe-4S]1+cluster Megalodesulfovibrio gigas
K+ stimulates, optimal concentration: 10 mM Megalodesulfovibrio gigas
Tungsten enzyme contains 0.68 gatom of tungsten per mol of subunit Megalodesulfovibrio gigas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 2 * 62000, SDS-PAGE Megalodesulfovibrio gigas
120000 126000 gel filtration Megalodesulfovibrio gigas

Organism

Organism UniProt Comment Textmining
Megalodesulfovibrio gigas
-
-
-
Megalodesulfovibrio gigas NCIMB 9332
-
-
-

Oxidation Stability

Oxidation Stability Organism
unstable to air Megalodesulfovibrio gigas

Purification (Commentary)

Purification (Comment) Organism
-
 Megalodesulfovibrio gigas

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38.5
-
-
 Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Methoxybenzaldehyde + benzyl viologen at 19% of the activity relative to acetaldehyde Megalodesulfovibrio gigas 4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
 ?
Acetaldehyde + 2,6-dichlorophenol-indophenol at 1.3% of the activity relative to benzyl viologen Megalodesulfovibrio gigas acetate + reduced 2,6-dichlorophenol-indophenol
-
 ?
Acetaldehyde + 2,6-dichlorophenol-indophenol at 1.3% of the activity relative to benzyl viologen Megalodesulfovibrio gigas NCIMB 9332 acetate + reduced 2,6-dichlorophenol-indophenol
-
 ?
Acetaldehyde + 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide at 5% of the activity relative to benzyl viologen Megalodesulfovibrio gigas acetate + reduced 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide
-
 ?
Acetaldehyde + 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide at 5% of the activity relative to benzyl viologen Megalodesulfovibrio gigas NCIMB 9332 acetate + reduced 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide
-
 ?
Acetaldehyde + benzyl viologen
-
 Megalodesulfovibrio gigas acetate + reduced benzyl viologen
-
 ?
Acetaldehyde + benzyl viologen
-
 Megalodesulfovibrio gigas NCIMB 9332 acetate + reduced benzyl viologen
-
 ?
Acetaldehyde + methyl viologen at 19% of the activity relative to benzyl viologen Megalodesulfovibrio gigas acetate + reduced methyl viologen
-
 ?
Acetaldehyde + methyl viologen at 19% of the activity relative to benzyl viologen Megalodesulfovibrio gigas NCIMB 9332 acetate + reduced methyl viologen
-
 ?
Benzaldehyde + benzyl viologen at 93% of the activity relative to acetaldehyde Megalodesulfovibrio gigas Benzoate + reduced benzyl viologen
-
 ?
Benzaldehyde + benzyl viologen at 93% of the activity relative to acetaldehyde Megalodesulfovibrio gigas NCIMB 9332 Benzoate + reduced benzyl viologen
-
 ?
Butyraldehyde + benzyl viologen at 16% of the activity relative to acetaldehyde Megalodesulfovibrio gigas Butanoate + reduced benzyl viologen
-
 ?
Furfural + benzyl viologen at 77% of the activity relative to acetaldehyde Megalodesulfovibrio gigas 2-Furancarboxylic acid + reduced benzyl viologen
-
 ?
Glutaraldehyde + benzyl viologen at 16% of the activity relative to acetaldehyde Megalodesulfovibrio gigas Glutarate + reduced benzyl viologen
-
 ?
Glycolaldehyde + benzyl viologen at 23% of the activity relative to acetaldehyde Megalodesulfovibrio gigas Glycolate + reduced benzyl viologen
-
 ?
Hexanal + benzyl viologen at 6% of the activity relative to acetaldehyde Megalodesulfovibrio gigas Hexanoate + reduced benzyl viologen
-
 ?
Methanal + benzyl viologen at 16% of the activity relative to acetaldehyde Megalodesulfovibrio gigas Formate + reduced benzyl viologen
-
 ?
Pentanal + benzyl viologen at 9% of the activity relative to acetaldehyde Megalodesulfovibrio gigas Pentanoate + reduced benzyl viologen
-
 ?
Phenylacetaldehyde + benzyl viologen at 31% of the activity relative to acetaldehyde Megalodesulfovibrio gigas Phenylacetate + reduced benzyl viologen
-
 ?
Propanal + benzyl viologen at 117% of the activity relative to acetaldehyde Megalodesulfovibrio gigas Propanoate + reduced benzyl viologen
-
 ?
Salicylaldehyde + benzyl viologen at 14% of the activity relative to acetaldehyde Megalodesulfovibrio gigas Salicylate + reduced benzyl viologen
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 62000, SDS-PAGE Megalodesulfovibrio gigas

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
48
-
 and above Megalodesulfovibrio gigas

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 decrease of activity after 3 min Megalodesulfovibrio gigas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 broad, with benzyl viologen and acetaldehyde as substrates Megalodesulfovibrio gigas

Cofactor

Cofactor Comment Organism Structure
molybdopterin enzyme contains molybdopterin Megalodesulfovibrio gigas