Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains one [4Fe-4S] cluster per subunit | Pyrococcus furiosus | |
Tungsten | one tungstobispterin cofactor per subunit | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
2 * 67000, SDS-PAGE | Pyrococcus furiosus |
135000 | - |
non-denaturing PAGE | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pyrococcus furiosus | ferredoxin can be the physiological electron acceptor | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | E7FHH1 and Q8U0V2 | E7FHH1: alpha-subunit, Q8U0V2: electron-transfer beta-subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.6 | - |
pH 8.4, 60°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ethylhexanal + H2O + oxidized benzyl viologen | Vmax/Km is 56% compared to the value for hexanal | Pyrococcus furiosus | 2-ethylhexanoate + reduced benzyl viologen + H+ | - |
? | |
2-methylbutyraldehyde + H2O + oxidized benzyl viologen | Vmax/Km is 20% compared to the value for hexanal | Pyrococcus furiosus | 2-methylbutyrate + reduced benzyl viologen + H+ | - |
? | |
2-methylvaleraldehyde + H2O + oxidized benzyl viologen | Vmax/Km is 54% compared to the value for hexanal | Pyrococcus furiosus | 2-methylvalerate + reduced benzyl viologen + H+ | - |
? | |
2-naphthaldehyde + H2O + oxidized benzyl viologen | Vmax/Km is 6.7% compared to the value for hexanal | Pyrococcus furiosus | 2-naphthoate + reduced benzyl viologen + H+ | - |
? | |
3-phenylbutyraldehyde + H2O + oxidized benzyl viologen | Vmax/Km is 11% compared to the value for hexanal | Pyrococcus furiosus | 3-phenylbutyrate + reduced benzyl viologen + H+ | - |
? | |
cinnamaldehyde + H2O + oxidized benzyl viologen | Vmax/Km is 5.3% compared to the value for hexanal | Pyrococcus furiosus | cinnamate + reduced benzyl viologen + H+ | - |
? | |
hexanal + H2O + 2 oxidized ferredoxin | - |
Pyrococcus furiosus | hexanoate + 2 reduced ferredoxin + 2 H+ | - |
? | |
hexanal + H2O + oxidized methyl viologen | - |
Pyrococcus furiosus | hexanoate + reduced methyl viologen + H+ | - |
? | |
hydratropaldehyde + H2O + oxidized benzyl viologen | Vmax/Km is 89% compared to the value for hexanal | Pyrococcus furiosus | ? + reduced benzyl viologen + H+ | - |
? | |
isobutyraldehyde + H2O + oxidized benzyl viologen | Vmax/Km is 17% compared to the value for hexanal | Pyrococcus furiosus | isobutyrate + reduced benzyl viologen + H+ | - |
? | |
additional information | ferredoxin can be the physiological electron acceptor | Pyrococcus furiosus | ? | - |
? | |
additional information | it is distinguished from aldehyde ferredoxin oxidoreductase (EC 1.2.7.5) by its low activity and affinity for crotonaldehyde, wich is one of the best substrates of aldehyde ferredoxin oxidoreductase. It is distinguished from formaldehyde ferredoxin oxidoreductase by its low activity and affinity for formaldehyde, which is one of the best substrates of formaldehyde ferredoxin oxidoreductase. It shows high affinity for several substituted and nonsubstituted aliphatic and aromatic aldehydes with various chain lengths. Very low activity (Vmax/Km is less than 5% compared to activity with hexanal): formaldehyde, crotonaldehyde, acetaldehyde, glutaraldehyde, 2-methoxybenzaldehyde. No activity with glyceraldehyde 3-phosphate | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 67000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
WOR5 | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Pyrococcus furiosus |