BRENDA - Enzyme Database
show all sequences of 1.2.7.B2

Redox chemistry of tungsten and iron-sulfur prosthetic groups in Pyrococcus furiosus formaldehyde ferredoxin oxidoreductase

Bol, E.; Bevers, L.E.; Hagedoorn, P.L.; Hagen, W.R.; J. Biol. Inorg. Chem. 11, 999-1006 (2006)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Sulfide
incubation of the enzyme (14 mg/ml) in 100 mM Tris/HCl (pH 8.0) buffer with excess sodium sulfide (20 mM) under anaerobic conditions leads to an enhancement of the specific activity by 40%
Pyrococcus furiosus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
oxidized ferredoxin
pH 8.4, 80°C
Pyrococcus furiosus
0.021
-
formaldehyde
pH 8.4, 80°C, with ferredoxin as electron acceptor
Pyrococcus furiosus
0.025
-
formaldehyde
pH 8.4, 20°C
Pyrococcus furiosus
0.033
-
formaldehyde
pH 8.4, 80°C
Pyrococcus furiosus
0.071
-
formaldehyde
pH 8.4, 80°C, with benzyl viologen as electron acceptor
Pyrococcus furiosus
0.073
-
Oxidized benzyl viologen
pH 8.4, 80°C
Pyrococcus furiosus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
contains one [4Fe-4S] cubane per 69000 Da subunit
Pyrococcus furiosus
Tungsten
contains one tungstodipterin per 69000 Da subunit; the tungsten in the active enzyme is a two-electron acceptor, W(VI/IV)
Pyrococcus furiosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus furiosus
-
-
-
Pyrococcus furiosus
Q8U1K3
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formaldehyde + H2O + 2 oxidized ferredoxin
-
674933
Pyrococcus furiosus
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
formaldehyde + H2O + oxidized benzyl viologen
-
674933
Pyrococcus furiosus
formate + H+ + reduced benzyl viologen
-
-
-
?
formaldehyde + H2O + oxidized benzyl viologen
no activity with methanol or formic acid as substrate
674933
Pyrococcus furiosus
formate + 2 H+ + reduced benzyl viologen
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
80
-
assay at
Pyrococcus furiosus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
14
-
formaldehyde
pH 8.4, 20°C
Pyrococcus furiosus
54
-
formaldehyde
pH 8.4, 80°C
Pyrococcus furiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at; assay at
Pyrococcus furiosus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Sulfide
incubation of the enzyme (14 mg/ml) in 100 mM Tris/HCl (pH 8.0) buffer with excess sodium sulfide (20 mM) under anaerobic conditions leads to an enhancement of the specific activity by 40%
Pyrococcus furiosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
oxidized ferredoxin
pH 8.4, 80°C
Pyrococcus furiosus
0.021
-
formaldehyde
pH 8.4, 80°C, with ferredoxin as electron acceptor
Pyrococcus furiosus
0.025
-
formaldehyde
pH 8.4, 20°C
Pyrococcus furiosus
0.033
-
formaldehyde
pH 8.4, 80°C
Pyrococcus furiosus
0.071
-
formaldehyde
pH 8.4, 80°C, with benzyl viologen as electron acceptor
Pyrococcus furiosus
0.073
-
Oxidized benzyl viologen
pH 8.4, 80°C
Pyrococcus furiosus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
contains one [4Fe-4S] cubane per 69000 Da subunit
Pyrococcus furiosus
Tungsten
the tungsten in the active enzyme is a two-electron acceptor, W(VI/IV)
Pyrococcus furiosus
Tungsten
contains one tungstodipterin per 69000 Da subunit
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formaldehyde + H2O + 2 oxidized ferredoxin
-
674933
Pyrococcus furiosus
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
formaldehyde + H2O + oxidized benzyl viologen
-
674933
Pyrococcus furiosus
formate + H+ + reduced benzyl viologen
-
-
-
?
formaldehyde + H2O + oxidized benzyl viologen
no activity with methanol or formic acid as substrate
674933
Pyrococcus furiosus
formate + 2 H+ + reduced benzyl viologen
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
80
-
assay at
Pyrococcus furiosus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
14
-
formaldehyde
pH 8.4, 20°C
Pyrococcus furiosus
54
-
formaldehyde
pH 8.4, 80°C
Pyrococcus furiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Pyrococcus furiosus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
560
-
formaldehyde
pH 8.4, 20°C
Pyrococcus furiosus
1636
-
formaldehyde
pH 8.4, 80°C
Pyrococcus furiosus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
560
-
formaldehyde
pH 8.4, 20°C
Pyrococcus furiosus
1636
-
formaldehyde
pH 8.4, 80°C
Pyrococcus furiosus
Other publictions for EC 1.2.7.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740555
Smalley
Functional and genomic diversi ...
Methyloversatilis discipulorum, Methyloversatilis discipulorum FAM1, Methyloversatilis discipulorum RZ18-153, Methyloversatilis discipulorum RZ94
Int. J. Syst. Evol. Microbiol.
65
2227-2233
2015
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4
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4
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722823
Liao
Why is the molybdenum-substitu ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
18
175-181
2013
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722893
Liao
Tungsten-dependent formaldehyd ...
Pyrococcus furiosus
J. Inorg. Biochem.
105
927-936
2011
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745210
Sevcenco
Molybdenum incorporation in t ...
Pyrococcus furiosus
J. Bacteriol.
192
4143-4152
2010
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1
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1
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723098
Sevcenco
The tungsten metallome of Pyro ...
Pyrococcus furiosus
Metallomics
1
395-402
2009
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674933
Bol
Redox chemistry of tungsten an ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
11
999-1006
2006
1
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2
674251
Bevers
WOR5, a novel tungsten-contain ...
Pyrococcus furiosus
J. Bacteriol.
187
7056-7061
2005
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3
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1
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644691
Roy
Aldehyde oxidoreductases from ...
Pyrococcus furiosus
Methods Enzymol.
331
132-144
2001
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7
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1
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9
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7
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9
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745202
Adams
Key role for sulfur in peptid ...
Pyrococcus furiosus
J. Bacteriol.
183
716-724
2001
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1
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722824
Dhawan
Spectroscopic studies of the t ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
J. Biol. Inorg. Chem.
5
313-327
2000
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644689
Roy
Purification and molecular cha ...
Pyrococcus furiosus
J. Bacteriol.
181
1171-1180
1999
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8
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1
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7
7
644690
Hu
Formaldehyde ferredoxin oxidor ...
Pyrococcus furiosus
J. Mol. Biol.
286
899-914
1999
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722490
Mukund
Molybdenum and vanadium do not ...
Pyrococcus furiosus
J. Bacteriol.
178
163-167
1996
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644684
Kletzin
Molecular characterization of ...
Thermococcus litoralis
J. Bacteriol.
177
4817-4819
1995
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644682
Mukund
Characterization of a novel tu ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
J. Biol. Chem.
268
13592-13600
1993
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