BRENDA - Enzyme Database show
show all sequences of 1.2.7.B2

Aldehyde oxidoreductases from Pyrococcus furiosus

Roy, R.; Menon, A.L.; Adams, M.W.W.; Methods Enzymol. 331, 132-144 (2001)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
glutaric dialdehyde
80C, pH 8.4
Pyrococcus furiosus
8
-
Succinic semialdehyde
80C, pH 8.4
Pyrococcus furiosus
15
-
phenylpropionaldehyde
80C, pH 8.4
Pyrococcus furiosus
25
-
formaldehyde
80C, pH 8.4
Pyrococcus furiosus
25
-
Indole-3-acetaldehyde
80C, pH 8.4
Pyrococcus furiosus
60
-
acetaldehyde
80C, pH 8.4
Pyrococcus furiosus
62
-
propionaldehyde
80C, pH 8.4
Pyrococcus furiosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus furiosus
O93738
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
acetate + H+ + reduced benzyl viologen
-
-
-
?
formaldehyde + H2O + 2 oxidized ferredoxin
-
644691
Pyrococcus furiosus
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
formaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
formate + H+ + reduced benzyl viologen
-
-
-
?
glutaric dialdehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
? + reduced benzyl viologen + H+
-
-
-
?
indole-3-acetaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
indole-3-acetate + H+ + reduced benzyl viologen
-
-
-
?
additional information
no activity with crotonaldehyde, benzaldehyde, isovalerylaldehyde or phenylacetaldehyde
644691
Pyrococcus furiosus
?
-
-
-
-
phenylpropionaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
phenylpropionate + H+ + reduced benzyl viologen
-
-
-
?
propionaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
propionate + H+ + reduced benzyl viologen
-
-
-
?
succinic semialdehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
succinate + H+ + reduced benzyl viologen
-
-
-
?
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
glutaric dialdehyde
80C, pH 8.4
Pyrococcus furiosus
8
-
Succinic semialdehyde
80C, pH 8.4
Pyrococcus furiosus
15
-
phenylpropionaldehyde
80C, pH 8.4
Pyrococcus furiosus
25
-
formaldehyde
80C, pH 8.4
Pyrococcus furiosus
25
-
Indole-3-acetaldehyde
80C, pH 8.4
Pyrococcus furiosus
60
-
acetaldehyde
80C, pH 8.4
Pyrococcus furiosus
62
-
propionaldehyde
80C, pH 8.4
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
acetate + H+ + reduced benzyl viologen
-
-
-
?
formaldehyde + H2O + 2 oxidized ferredoxin
-
644691
Pyrococcus furiosus
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
formaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
formate + H+ + reduced benzyl viologen
-
-
-
?
glutaric dialdehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
? + reduced benzyl viologen + H+
-
-
-
?
indole-3-acetaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
indole-3-acetate + H+ + reduced benzyl viologen
-
-
-
?
additional information
no activity with crotonaldehyde, benzaldehyde, isovalerylaldehyde or phenylacetaldehyde
644691
Pyrococcus furiosus
?
-
-
-
-
phenylpropionaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
phenylpropionate + H+ + reduced benzyl viologen
-
-
-
?
propionaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
propionate + H+ + reduced benzyl viologen
-
-
-
?
succinic semialdehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
succinate + H+ + reduced benzyl viologen
-
-
-
?
Other publictions for EC 1.2.7.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740555
Smalley
Functional and genomic diversi ...
Methyloversatilis discipulorum, Methyloversatilis discipulorum FAM1, Methyloversatilis discipulorum RZ18-153, Methyloversatilis discipulorum RZ94
Int. J. Syst. Evol. Microbiol.
65
2227-2233
2015
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4
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10
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4
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-
722823
Liao
Why is the molybdenum-substitu ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
18
175-181
2013
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1
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722893
Liao
Tungsten-dependent formaldehyd ...
Pyrococcus furiosus
J. Inorg. Biochem.
105
927-936
2011
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745210
Sevcenco
Molybdenum incorporation in t ...
Pyrococcus furiosus
J. Bacteriol.
192
4143-4152
2010
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1
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1
1
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1
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1
1
1
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723098
Sevcenco
The tungsten metallome of Pyro ...
Pyrococcus furiosus
Metallomics
1
395-402
2009
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1
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3
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1
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1
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674933
Bol
Redox chemistry of tungsten an ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
11
999-1006
2006
1
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6
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2
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5
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1
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1
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1
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6
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3
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3
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1
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2
2
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2
2
674251
Bevers
WOR5, a novel tungsten-contain ...
Pyrococcus furiosus
J. Bacteriol.
187
7056-7061
2005
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3
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1
1
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644691
Roy
Aldehyde oxidoreductases from ...
Pyrococcus furiosus
Methods Enzymol.
331
132-144
2001
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7
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1
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9
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7
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9
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745202
Adams
Key role for sulfur in peptid ...
Pyrococcus furiosus
J. Bacteriol.
183
716-724
2001
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1
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1
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722824
Dhawan
Spectroscopic studies of the t ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
J. Biol. Inorg. Chem.
5
313-327
2000
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644689
Roy
Purification and molecular cha ...
Pyrococcus furiosus
J. Bacteriol.
181
1171-1180
1999
1
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3
8
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2
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1
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9
1
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7
1
1
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1
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3
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8
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2
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1
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1
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9
1
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7
1
1
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7
7
644690
Hu
Formaldehyde ferredoxin oxidor ...
Pyrococcus furiosus
J. Mol. Biol.
286
899-914
1999
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5
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722490
Mukund
Molybdenum and vanadium do not ...
Pyrococcus furiosus
J. Bacteriol.
178
163-167
1996
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1
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644684
Kletzin
Molecular characterization of ...
Thermococcus litoralis
J. Bacteriol.
177
4817-4819
1995
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644682
Mukund
Characterization of a novel tu ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
J. Biol. Chem.
268
13592-13600
1993
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3
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2
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8
1
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11
1
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11
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