Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
CoA | - |
Pyrococcus furiosus | |
0.048 | - |
Ferredoxin | - |
Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
24000 | - |
2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
66000 | - |
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
71000 | - |
2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Methanothermobacter thermautotrophicus | - |
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Pyrococcus furiosus | - |
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Pyrococcus furiosus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
irreversibly inactivated by oxygen | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Methanothermobacter thermautotrophicus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+ | - |
r | |
2-keto-4-methylthiobutyrate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | CO2 + reduced ferredoxin + 3-methylthiopropionyl-CoA | - |
r | |
2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | CO2 + reduced ferredoxin + isovaleryl-CoA | - |
r | |
indolepyruvate + CoA + oxidized methylviologen | - |
Pyrococcus furiosus | indoleacetyl-CoA + CO2 + reduced methylviologen | - |
? | |
additional information | pyruvate, 2-ketoisovalerate, 2-ketoglutarate, 2 ketobutyrate, and phenylglyoxylate are no substrates | Pyrococcus furiosus | ? | - |
? | |
p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
phenylpyruvate + CoA + oxidized ferredoxin | - |
Methanothermobacter thermautotrophicus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Methanothermobacter thermautotrophicus |
tetramer | 2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
tetramer | 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |