BRENDA - Enzyme Database show
show all sequences of 1.2.7.7

Thermococcus profundus 2-ketoisovalerate ferredoxin oxidoreductase, a key enzyme in the archaeal energy-producing amino acid metabolic pathway

Ozawa, Y.; Nakamura, T.; Kamata, N.; Yasujima, D.; Urushiyama, A.; Yamakura, F.; Ohmori, D.; Imai, T.; J. Biochem. 137, 101-107 (2005)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
coenzyme A
-
Thermococcus profundus
0.17
-
2-ketoisovalerate
-
Thermococcus profundus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
4Fe-4S cluster
-
Thermococcus profundus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
13000
-
delta subunit, determined by SDS-PAGE
Thermococcus profundus
22000
-
gamma subunit, determined by SDS-PAGE
Thermococcus profundus
31000
-
beta subunit, determined by SDS-PAGE
Thermococcus profundus
45000
-
alpha subunit, determined by SDS-PAGE
Thermococcus profundus
218000
-
native enzyme, determined by gel filtration
Thermococcus profundus
220000
-
native enzyme, determined by analytical centrifugation
Thermococcus profundus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-methyl-2-oxobutanoate + CoA + oxidized ferredoxin
Thermococcus profundus
-
S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus profundus
-
-
-
Purification (Commentary)
Commentary
Organism
cell-free extracts are loaded onto a Q Sepharose Fast Flow column, activity containing fractions are loaded onto a hydroxyapatite column, finally a TOYOPEARL column is used
Thermococcus profundus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.76
-
cell-free extract
Thermococcus profundus
3.8
-
Hydroxyapatite purified
Thermococcus profundus
4.3
-
Q Sepharose purified
Thermococcus profundus
128
-
Red TOYOPEARL purified
Thermococcus profundus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-ketoisovalerate + CoA + oxidized ferredoxin
-
680533
Thermococcus profundus
?
-
-
-
?
3-methyl-2-oxobutanoate + CoA + oxidized ferredoxin
-
680533
Thermococcus profundus
S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
-
-
?
Subunits
Subunits
Commentary
Organism
octamer
heterooctamer consisting of four different subunits
Thermococcus profundus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
80
-
activity assay, VOR activity is routinely assayed by means of CoA-dependent reduction of methyl viologen with 2-ketoisovalerate at 80C
Thermococcus profundus
93
-
the optimal temperature for the 2-ketoisovalerate methyl viologen oxidoreductase assay is determined to be 93C
Thermococcus profundus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
80
112
-
Thermococcus profundus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
activity assay
Thermococcus profundus
Cofactor
Cofactor
Commentary
Organism
Structure
CoA
-
Thermococcus profundus
Ferredoxin
-
Thermococcus profundus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
CoA
-
Thermococcus profundus
Ferredoxin
-
Thermococcus profundus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
coenzyme A
-
Thermococcus profundus
0.17
-
2-ketoisovalerate
-
Thermococcus profundus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
4Fe-4S cluster
-
Thermococcus profundus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
13000
-
delta subunit, determined by SDS-PAGE
Thermococcus profundus
22000
-
gamma subunit, determined by SDS-PAGE
Thermococcus profundus
31000
-
beta subunit, determined by SDS-PAGE
Thermococcus profundus
45000
-
alpha subunit, determined by SDS-PAGE
Thermococcus profundus
218000
-
native enzyme, determined by gel filtration
Thermococcus profundus
220000
-
native enzyme, determined by analytical centrifugation
Thermococcus profundus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-methyl-2-oxobutanoate + CoA + oxidized ferredoxin
Thermococcus profundus
-
S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
cell-free extracts are loaded onto a Q Sepharose Fast Flow column, activity containing fractions are loaded onto a hydroxyapatite column, finally a TOYOPEARL column is used
Thermococcus profundus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.76
-
cell-free extract
Thermococcus profundus
3.8
-
Hydroxyapatite purified
Thermococcus profundus
4.3
-
Q Sepharose purified
Thermococcus profundus
128
-
Red TOYOPEARL purified
Thermococcus profundus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-ketoisovalerate + CoA + oxidized ferredoxin
-
680533
Thermococcus profundus
?
-
-
-
?
3-methyl-2-oxobutanoate + CoA + oxidized ferredoxin
-
680533
Thermococcus profundus
S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
octamer
heterooctamer consisting of four different subunits
Thermococcus profundus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
80
-
activity assay, VOR activity is routinely assayed by means of CoA-dependent reduction of methyl viologen with 2-ketoisovalerate at 80C
Thermococcus profundus
93
-
the optimal temperature for the 2-ketoisovalerate methyl viologen oxidoreductase assay is determined to be 93C
Thermococcus profundus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
80
112
-
Thermococcus profundus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
activity assay
Thermococcus profundus
Other publictions for EC 1.2.7.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736738
Lin
Consolidated bioprocessing of ...
Hungateiclostridium thermocellum, Hungateiclostridium thermocellum DSM 1313
Metab. Eng.
31
44-52
2015
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736364
Nohara
Genetic examination and mass b ...
Thermococcus kodakarensis, Thermococcus kodakarensis KUW1
J. Bacteriol.
196
3831-3839
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680533
Ozawa
Thermococcus profundus 2-ketoi ...
Thermococcus profundus
J. Biochem.
137
101-107
2005
-
-
-
-
-
-
-
2
-
1
6
1
-
4
-
-
1
-
-
-
4
-
2
1
2
1
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
6
1
-
-
-
1
-
-
4
-
2
1
2
1
-
-
1
-
-
-
-
-
-
-
-
-
639362
Schut
2-Keto acid oxidoreductases fr ...
Pyrococcus furiosus, Thermococcus litoralis
Methods Enzymol.
331
144-158
2001
2
-
1
-
-
-
-
2
-
2
4
2
-
2
1
-
1
2
-
-
-
-
24
1
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
2
-
2
4
2
-
1
-
1
-
-
-
-
24
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644697
Tersteegen
Structures and functions of fo ...
Methanothermobacter thermautotrophicus, Pyrococcus furiosus, Thermococcus litoralis
Eur. J. Biochem.
244
862-868
1997
-
-
-
-
-
-
-
2
-
-
3
4
-
8
-
-
1
3
-
-
1
-
18
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
4
-
-
-
1
-
-
1
-
18
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288443
Kletzin
Molecular and phylogenetic cha ...
Pyrococcus furiosus
J. Bacteriol.
178
248-257
1996
1
-
1
-
-
-
-
-
-
1
4
1
-
7
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
4
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644696
Heider
Characterization of 2-ketoisov ...
Pyrococcus furiosus, Pyrococcus sp., Pyrococcus sp. ES-4, Thermococcus litoralis, Thermococcus sp., Thermococcus sp. ES-1
J. Bacteriol.
178
780-787
1996
3
-
-
-
-
-
2
22
-
3
9
5
-
14
1
-
4
4
-
-
2
-
72
2
2
2
2
24
1
-
1
-
-
-
-
3
-
-
-
-
-
-
-
2
-
22
-
3
9
5
-
1
-
4
-
-
2
-
72
2
2
2
2
24
1
-
1
-
-
-
-
-
-
-