KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
coenzyme A | - |
Thermococcus profundus | |
0.17 | - |
2-ketoisovalerate | - |
Thermococcus profundus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S cluster | - |
Thermococcus profundus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
delta subunit, determined by SDS-PAGE | Thermococcus profundus |
22000 | - |
gamma subunit, determined by SDS-PAGE | Thermococcus profundus |
31000 | - |
beta subunit, determined by SDS-PAGE | Thermococcus profundus |
45000 | - |
alpha subunit, determined by SDS-PAGE | Thermococcus profundus |
218000 | - |
native enzyme, determined by gel filtration | Thermococcus profundus |
220000 | - |
native enzyme, determined by analytical centrifugation | Thermococcus profundus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoate + CoA + oxidized ferredoxin | Thermococcus profundus | - |
S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus profundus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cell-free extracts are loaded onto a Q Sepharose Fast Flow column, activity containing fractions are loaded onto a hydroxyapatite column, finally a TOYOPEARL column is used | Thermococcus profundus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.76 | - |
cell-free extract | Thermococcus profundus |
3.8 | - |
Hydroxyapatite purified | Thermococcus profundus |
4.3 | - |
Q Sepharose purified | Thermococcus profundus |
128 | - |
Red TOYOPEARL purified | Thermococcus profundus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ketoisovalerate + CoA + oxidized ferredoxin | - |
Thermococcus profundus | ? | - |
? | |
3-methyl-2-oxobutanoate + CoA + oxidized ferredoxin | - |
Thermococcus profundus | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | heterooctamer consisting of four different subunits | Thermococcus profundus |
Synonyms | Comment | Organism |
---|---|---|
2-ketoisovalerate ferredoxin oxidoreductase | - |
Thermococcus profundus |
3-methyl-2-oxobutanoate dehydrogenase | - |
Thermococcus profundus |
VOR | - |
Thermococcus profundus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
activity assay, VOR activity is routinely assayed by means of CoA-dependent reduction of methyl viologen with 2-ketoisovalerate at 80°C | Thermococcus profundus |
93 | - |
the optimal temperature for the 2-ketoisovalerate methyl viologen oxidoreductase assay is determined to be 93°C | Thermococcus profundus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 112 | - |
Thermococcus profundus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
activity assay | Thermococcus profundus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Thermococcus profundus | |
Ferredoxin | - |
Thermococcus profundus |