Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,3-bisphosphoglycerate | stimulates | Pyrococcus furiosus | |
3-phosphoglycerate | stimulates | Pyrococcus furiosus | |
acetyl phosphate | stimulates | Pyrococcus furiosus | |
Ionic strength | particularly by polyanions | Pyrococcus furiosus | |
potassium chloride | 3.5fold activation at 200 mM | Pyrococcus furiosus | |
potassium phosphate | 3.5fold activation at 200 mM | Pyrococcus furiosus | |
Sodium arsenate | 3.5fold activation at 200 mM | Pyrococcus furiosus | |
Sodium citrate | 3.5fold activation at 200 mM | Pyrococcus furiosus | |
Sodium sulfate | 3.5fold activation at 200 mM | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
crotonaldehyde | 25% inhibition at 10 mM | Pyrococcus furiosus | |
Dithionite | 50% inhibition at 3 mM, pH 8.0; O2-scavenger; reversible by 70% | Pyrococcus furiosus | |
formaldehyde | 25% inhibition at 10 mM | Pyrococcus furiosus | |
glyceraldehyde-3-phosphate | substrate inhibition above 0.5 mM | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
oxidized ferredoxin | pH 8.4, 70°C | Pyrococcus furiosus | |
0.028 | - |
D-Glyceraldehyde-3-phosphate | with ferredoxin as electron acceptor, pH 8.4, 70°C | Pyrococcus furiosus | |
0.043 | - |
D-Glyceraldehyde-3-phosphate | with benzyl viologen as electron acceptor, pH 8.4, 70°C | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pyrococcus furiosus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | enzyme contains iron-sulfur cluster | Pyrococcus furiosus | |
Fe2+ | 5-6 iron g-atoms per mol of enzyme | Pyrococcus furiosus | |
Tungsten | 0.85 tungsten g-atoms per mol of enzyme, part of a tungsten-molybdopterin cofactor | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Pyrococcus furiosus |
63000 | - |
1 * 63000, SDS-PAGE | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | Pyrococcus furiosus | might have a glycolytic role and functions in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase | 3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
hyperthermophilic archaeon | - |
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Oxidation Stability | Organism |
---|---|
aerobic conditions: loss of about 50% activity in cell extract after 12 h at 23°C | Pyrococcus furiosus |
sensitive against O2 | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
30fold, under strictly anaerobic conditions | Pyrococcus furiosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin = 3-phospho-D-glycerate + 2 H+ + reduced ferredoxin | this enzyme is thought to function in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase in the novel Embden-Meyerhoff-type glycolytic pathway found in Pyrococcus furiosus. It is specific for glyceraldehyde-3-phosphate | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | - |
purified enzyme | Pyrococcus furiosus |
Storage Stability | Organism |
---|---|
23°C, under argon, 12 h, no loss of activity in cell extract | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen | artificial electron acceptor | Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced benzyl viologen | - |
? | |
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? | |
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | might have a glycolytic role and functions in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase | Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? | |
additional information | no activity with NAD(P)+ as electron acceptor | Pyrococcus furiosus | ? | - |
? | |
additional information | no activity with formaldehyde, propionaldehyde, phenylacetaldehyde, butyraldehyde, crotonaldehyde, acetaldehyde, dihydroxyacetone phosphate, glyceraldehyde, benzaldehyde, glucose, glucose 6-phosphate, or glyoxylate | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
glyceraldehyde-3-phosphate ferredoxin oxidoreductase | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Pyrococcus furiosus | |
tungsten-molybdopterin | tungstopterin center | Pyrococcus furiosus |