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Literature summary for 1.2.7.6 extracted from

  • Mukund, S.; Adams, M.W.
    Glyceraldehyde-3-phosphate ferredoxin oxidoreductase, a novel tungsten-containing enzyme with a potential glycolytic role in the hyperthermophilic archaeon Pyrococcus furiosus (1995), J. Biol. Chem., 270, 8389-8392.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2,3-bisphosphoglycerate stimulates Pyrococcus furiosus
3-phosphoglycerate stimulates Pyrococcus furiosus
acetyl phosphate stimulates Pyrococcus furiosus
Ionic strength particularly by polyanions Pyrococcus furiosus
potassium chloride 3.5fold activation at 200 mM Pyrococcus furiosus
potassium phosphate 3.5fold activation at 200 mM Pyrococcus furiosus
Sodium arsenate 3.5fold activation at 200 mM Pyrococcus furiosus
Sodium citrate 3.5fold activation at 200 mM Pyrococcus furiosus
Sodium sulfate 3.5fold activation at 200 mM Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
crotonaldehyde 25% inhibition at 10 mM Pyrococcus furiosus
Dithionite 50% inhibition at 3 mM, pH 8.0; O2-scavenger; reversible by 70% Pyrococcus furiosus
formaldehyde 25% inhibition at 10 mM Pyrococcus furiosus
glyceraldehyde-3-phosphate substrate inhibition above 0.5 mM Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
oxidized ferredoxin pH 8.4, 70°C Pyrococcus furiosus
0.028
-
D-Glyceraldehyde-3-phosphate with ferredoxin as electron acceptor, pH 8.4, 70°C Pyrococcus furiosus
0.043
-
D-Glyceraldehyde-3-phosphate with benzyl viologen as electron acceptor, pH 8.4, 70°C Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Pyrococcus furiosus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ enzyme contains iron-sulfur cluster Pyrococcus furiosus
Fe2+ 5-6 iron g-atoms per mol of enzyme Pyrococcus furiosus
Tungsten 0.85 tungsten g-atoms per mol of enzyme, part of a tungsten-molybdopterin cofactor Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Pyrococcus furiosus
63000
-
1 * 63000, SDS-PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin Pyrococcus furiosus might have a glycolytic role and functions in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
hyperthermophilic archaeon
-
Pyrococcus furiosus
-
strain DSM 3638
-

Oxidation Stability

Oxidation Stability Organism
aerobic conditions: loss of about 50% activity in cell extract after 12 h at 23°C Pyrococcus furiosus
sensitive against O2 Pyrococcus furiosus

Purification (Commentary)

Purification (Comment) Organism
30fold, under strictly anaerobic conditions Pyrococcus furiosus

Reaction

Reaction Comment Organism Reaction ID
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin = 3-phospho-D-glycerate + 2 H+ + reduced ferredoxin this enzyme is thought to function in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase in the novel Embden-Meyerhoff-type glycolytic pathway found in Pyrococcus furiosus. It is specific for glyceraldehyde-3-phosphate Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
140
-
purified enzyme Pyrococcus furiosus

Storage Stability

Storage Stability Organism
23°C, under argon, 12 h, no loss of activity in cell extract Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen artificial electron acceptor Pyrococcus furiosus 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
?
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin
-
Pyrococcus furiosus 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
?
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin might have a glycolytic role and functions in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase Pyrococcus furiosus 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
?
additional information no activity with NAD(P)+ as electron acceptor Pyrococcus furiosus ?
-
?
additional information no activity with formaldehyde, propionaldehyde, phenylacetaldehyde, butyraldehyde, crotonaldehyde, acetaldehyde, dihydroxyacetone phosphate, glyceraldehyde, benzaldehyde, glucose, glucose 6-phosphate, or glyoxylate Pyrococcus furiosus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 63000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
glyceraldehyde-3-phosphate ferredoxin oxidoreductase
-
Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
assay at Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
Pyrococcus furiosus
tungsten-molybdopterin tungstopterin center Pyrococcus furiosus