BRENDA - Enzyme Database show
show all sequences of 1.2.7.5

Purification and characterization of the tungsten enzyme aldehyde:ferredoxin oxidoreductase from the hyperthermophilic denitrifier Pyrobaculum aerophilum

Hagedoorn, P.L.; Chen, T.; Schroder, I.; Piersma, S.R.; Vries, S.D.; Hagen, W.R.; J. Biol. Inorg. Chem. 10, 259-269 (2005)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
crotonaldehyde
pH 8.4, 60°C
Pyrobaculum aerophilum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
contains 3.3 mol Fe per mol of monomer, the enzyme contains a single [4Fe-4S]2+/1+ cluster per monomer
Pyrobaculum aerophilum
additional information
contains no detectable molybdenum
Pyrobaculum aerophilum
Tungsten
contains 0.7 mol tungsten per mol of monomer, the enzyme contains a single tungstopterin cofactor
Pyrobaculum aerophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
2 * 68000, SDS-PAGE
Pyrobaculum aerophilum
130000
-
gel filtration
Pyrobaculum aerophilum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrobaculum aerophilum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pyrobaculum aerophilum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.5
-
-
Pyrobaculum aerophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized benzyl viologen
at 5% of the activity with crotonaldehyde
656293
Pyrobaculum aerophilum
acetate + reduced benzyl viologen
-
-
-
?
benzaldehyde + H2O + oxidized benzyl viologen
at 149% of the activity with crotonaldehyde
656293
Pyrobaculum aerophilum
benzoate + reduced benzyl viologen
-
-
-
?
cinnamaldehyde + H2O + oxidized benzyl viologen
at 139% of the activity with crotonaldehyde
656293
Pyrobaculum aerophilum
cinnamate + reduced benzyl viologen
-
-
-
?
crotonaldehyde + H2O + oxidized benzyl viologen
-
656293
Pyrobaculum aerophilum
crotonate + reduced benzyl viologen
-
-
-
?
crotonaldehyde + H2O + oxidized ferredoxin
utilizes a 7Fe ferredoxin as the putative physiological redox partner, instead of a 4Fe ferredoxin as in Pyrococcus furiosus, 17% of the activity with benzyl viologen
656293
Pyrobaculum aerophilum
crotonate + reduced ferredoxin
-
-
-
?
formaldehyde + H2O + oxidized benzyl viologen
-
656293
Pyrobaculum aerophilum
formate + reduced benzyl viologen
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 68000, SDS-PAGE
Pyrobaculum aerophilum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
100
-
-
Pyrobaculum aerophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
crotonaldehyde
pH 8.4, 60°C
Pyrobaculum aerophilum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
contains 3.3 mol Fe per mol of monomer, the enzyme contains a single [4Fe-4S]2+/1+ cluster per monomer
Pyrobaculum aerophilum
additional information
contains no detectable molybdenum
Pyrobaculum aerophilum
Tungsten
contains 0.7 mol tungsten per mol of monomer, the enzyme contains a single tungstopterin cofactor
Pyrobaculum aerophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
2 * 68000, SDS-PAGE
Pyrobaculum aerophilum
130000
-
gel filtration
Pyrobaculum aerophilum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pyrobaculum aerophilum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.5
-
-
Pyrobaculum aerophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized benzyl viologen
at 5% of the activity with crotonaldehyde
656293
Pyrobaculum aerophilum
acetate + reduced benzyl viologen
-
-
-
?
benzaldehyde + H2O + oxidized benzyl viologen
at 149% of the activity with crotonaldehyde
656293
Pyrobaculum aerophilum
benzoate + reduced benzyl viologen
-
-
-
?
cinnamaldehyde + H2O + oxidized benzyl viologen
at 139% of the activity with crotonaldehyde
656293
Pyrobaculum aerophilum
cinnamate + reduced benzyl viologen
-
-
-
?
crotonaldehyde + H2O + oxidized benzyl viologen
-
656293
Pyrobaculum aerophilum
crotonate + reduced benzyl viologen
-
-
-
?
crotonaldehyde + H2O + oxidized ferredoxin
utilizes a 7Fe ferredoxin as the putative physiological redox partner, instead of a 4Fe ferredoxin as in Pyrococcus furiosus, 17% of the activity with benzyl viologen
656293
Pyrobaculum aerophilum
crotonate + reduced ferredoxin
-
-
-
?
formaldehyde + H2O + oxidized benzyl viologen
-
656293
Pyrobaculum aerophilum
formate + reduced benzyl viologen
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 68000, SDS-PAGE
Pyrobaculum aerophilum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
100
-
-
Pyrobaculum aerophilum
Other publictions for EC 1.2.7.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740953
Liew
Metabolic engineering of Clost ...
Clostridium autoethanogenum
Metab. Eng.
40
104-114
2017
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1
1
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740209
Ammam
Effect of tungstate on acetate ...
Sporomusa ovata, Sporomusa ovata DSM-2662
Biotechnol. Biofuels
9
163
2016
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737164
Basen
Single gene insertion drives b ...
Pyrococcus furiosus
Proc. Natl. Acad. Sci. USA
111
17618-17623
2014
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740626
Debnar-Daumler
Simultaneous involvement of a ...
Aromatoleum aromaticum
J. Bacteriol.
196
483-492
2014
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2
1
1
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1
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8
1
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722823
Liao
Why is the molybdenum-substitu ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
18
175-181
2013
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2
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1
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722893
Liao
Tungsten-dependent formaldehyd ...
Pyrococcus furiosus
J. Inorg. Biochem.
105
927-936
2011
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1
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745210
Sevcenco
Molybdenum incorporation in t ...
Pyrococcus furiosus
J. Bacteriol.
192
4143-4152
2010
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1
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692610
Sugimoto
In situ generation of oxo-sulf ...
synthetic construct
Inorg. Chem.
46
8460-8462
2007
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1
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675237
Thapper
Biochemical and spectroscopic ...
Desulfovibrio aminophilus, Desulfovibrio aminophilus DSM 12254
J. Inorg. Biochem.
100
44-50
2006
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2
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1
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656293
Hagedoorn
Purification and characterizat ...
Pyrobaculum aerophilum
J. Biol. Inorg. Chem.
10
259-269
2005
-
-
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-
1
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3
2
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4
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1
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1
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6
1
1
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1
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3
2
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1
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1
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6
1
1
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674251
Bevers
WOR5, a novel tungsten-contain ...
Pyrococcus furiosus
J. Bacteriol.
187
7056-7061
2005
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1
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654430
Wahyudi
Characterization of aldehyde f ...
Magnetospirillum magneticum, Magnetospirillum magneticum AMB-1
Biochem. Biophys. Res. Commun.
303
223-229
2003
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644691
Roy
Aldehyde oxidoreductases from ...
Pyrococcus endeavori, Pyrococcus furiosus, Thermococcus litoralis, Thermococcus sp., Thermococcus sp. ES1
Methods Enzymol.
331
132-144
2001
3
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15
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11
5
5
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2
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40
2
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1
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12
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15
1
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15
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13
5
5
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2
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40
3
4
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1
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4
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745202
Adams
Key role for sulfur in peptid ...
Pyrococcus furiosus
J. Bacteriol.
183
716-724
2001
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644689
Roy
Purification and molecular cha ...
Pyrococcus furiosus
J. Bacteriol.
181
1171-1180
1999
1
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644690
Hu
Formaldehyde ferredoxin oxidor ...
Pyrococcus furiosus
J. Mol. Biol.
286
899-914
1999
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721578
Zhou
Site-directed mutations of the ...
Pyrococcus furiosus
Biochemistry
36
10892-10900
1997
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1
644687
Koehler
-
Spectroscopic characterization ...
Pyrococcus endeavori, Pyrococcus furiosus
J. Am. Chem. Soc.
118
12391-12405
1996
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6
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6
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644688
Das
-
Modeling the tungsten sites of ...
Pyrococcus furiosus
J. Am. Chem. Soc.
118
1387-1397
1996
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722057
Arendsen
-
Redox chemistry of biological ...
Pyrococcus furiosus
Chemistry
1
292-296
1996
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2
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1
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1
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Molybdenum and vanadium do not ...
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Purification, characterization ...
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16
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15
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11
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6
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16
1
1
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2
15
1
1
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Kletzin
Molecular characterization of ...
Pyrococcus furiosus
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4
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644686
Chan
Structure of a hyperthermophil ...
Pyrococcus furiosus
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3
2
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3
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722438
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Aldehyde ferredoxin oxidoreduc ...
Pyrococcus furiosus
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11938
Mukund
The novel tungsten-iron-sulfur ...
Pyrococcus furiosus
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1
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