BRENDA - Enzyme Database
show all sequences of 1.2.7.5

Aldehyde oxidoreductases from Pyrococcus furiosus

Roy, R.; Menon, A.L.; Adams, M.W.W.; Methods Enzymol. 331, 132-144 (2001)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Sulfide
4-5fold increase in activity at room temperature and pH 8.0 at 20 mM, in presence of 20 mM dithionite; activation of formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
Tungsten
stimulates cell growth
Pyrococcus furiosus
Tungsten
stimulates cell growth
Thermococcus litoralis
Crystallization (Commentary)
Crystallization
Organism
-
Pyrococcus furiosus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
acetaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.028
-
isovalerylaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.05
-
Indoleacetaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.057
-
benzaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.076
-
phenylacetaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.14
-
crotonaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.15
-
propionaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.8
-
glutaric dialdehyde
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
Pyrococcus furiosus
1.42
-
formaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
8
-
Succinic semialdehyde
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
Pyrococcus furiosus
15
-
phenylpropionaldehyde
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
Pyrococcus furiosus
25
-
formaldehyde
formaldehyde ferredoxin oxidoreductase, pH 8.4, 80°C, with benzyl viologen as electron acceptor
Pyrococcus furiosus
25
-
Indoleacetaldehyde
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
Pyrococcus furiosus
60
-
acetaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Pyrococcus furiosus
60
-
propionaldehyde
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
Pyrococcus furiosus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 Ca2+ per subunit, formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
Fe2+
contains 1 iron-sulfur cluster [4Fe-4S] per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus endeavori
Fe2+
contains 1 additional Fe2+ besides the [4Fe-4S]-cluster; contains 1 iron-sulfur cluster [4Fe-4S] per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus furiosus
Fe2+
contains 1 iron-sulfur cluster [4Fe-4S] per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus litoralis
Fe2+
contains 1 iron-sulfur cluster [4Fe-4S] per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus sp.
Mg2+
both enzyme forms; contains 1 Mg-atom per subunit
Pyrococcus furiosus
Mg2+
contains 1 Mg-atom per subunit
Thermococcus sp.
Tungsten
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus endeavori
Tungsten
1 tungsten per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus furiosus
Tungsten
1 tungsten per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus litoralis
Tungsten
1 tungsten per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
2 * 67000
Pyrococcus furiosus
67000
-
2 * 67000, SDS-PAGE
Thermococcus sp.
136100
-
-
Pyrococcus furiosus
280000
-
-
Pyrococcus furiosus
280000
-
-
Thermococcus litoralis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
an aldehyde + H2O + oxidized ferredoxin
Thermococcus sp.
-
an acid + H+ + reduced ferredoxin
-
Thermococcus sp.
?
an aldehyde + H2O + oxidized ferredoxin
Pyrococcus endeavori
-
an acid + H+ + reduced ferredoxin
-
Pyrococcus endeavori
?
an aldehyde + H2O + oxidized ferredoxin
Pyrococcus furiosus
formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea
an acid + H+ + reduced ferredoxin
-
Pyrococcus furiosus
?
an aldehyde + H2O + oxidized ferredoxin
Thermococcus litoralis
formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea
an acid + H+ + reduced ferredoxin
-
Thermococcus litoralis
?
an aldehyde + H2O + oxidized ferredoxin
Thermococcus sp. ES1
-
an acid + H+ + reduced ferredoxin
-
Thermococcus sp. ES1
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus endeavori
-
hyperthermophilic archaeon
-
Pyrococcus furiosus
-
contains a formaldehyde and an aldehyde ferredoxin oxidoreductase; hyperthermophilic archaeon; strain DSM 3638
-
Pyrococcus furiosus
Q51739
gene aor; hyperthermophilic archaeon; strain DSM 3638
-
Thermococcus litoralis
-
formaldehyde ferredoxin oxidoreductase; hyperthermophilic archaeon
-
Thermococcus sp.
-
hyperthermophilic archaeon
-
Thermococcus sp. ES1
-
hyperthermophilic archaeon
-
Oxidation Stability
Oxidation Stability
Organism
aldehyde ferredoxin oxidoreductase, t1/2: 30 min at 23 °C
Pyrococcus furiosus
formaldehyde ferredoxin oxidoreductase, t1/2: 9 h at 23°C
Pyrococcus furiosus
Purification (Commentary)
Commentary
Organism
formaldehyde and aldehyde ferredoxin oxidoreductase; formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required
Pyrococcus furiosus
formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required
Thermococcus litoralis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
acetate + H+ + reduced benzyl viologen
-
644691
Thermococcus sp.
ir
acetaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Pyrococcus furiosus
acetate + H+ + reduced benzyl viologen
-
644691
Pyrococcus furiosus
ir
acetaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
acetate + H+ + reduced benzyl viologen
-
644691
Thermococcus litoralis
ir
acetaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp. ES1
acetate + H+ + reduced benzyl viologen
-
644691
Thermococcus sp. ES1
ir
an aldehyde + H2O + oxidized ferredoxin
-
644691
Pyrococcus furiosus
an acid + H+ + reduced ferredoxin
-
644691
Pyrococcus furiosus
ir
an aldehyde + H2O + oxidized ferredoxin
-
644691
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus sp.
?
an aldehyde + H2O + oxidized ferredoxin
-
644691
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus sp.
ir
an aldehyde + H2O + oxidized ferredoxin
-
644691
Thermococcus litoralis
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus litoralis
ir
an aldehyde + H2O + oxidized ferredoxin
-
644691
Pyrococcus endeavori
an acid + H+ + reduced ferredoxin
-
644691
Pyrococcus endeavori
?
an aldehyde + H2O + oxidized ferredoxin
-
644691
Pyrococcus endeavori
an acid + H+ + reduced ferredoxin
-
644691
Pyrococcus endeavori
ir
an aldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea
644691
Pyrococcus furiosus
an acid + H+ + reduced ferredoxin
-
644691
Pyrococcus furiosus
?
an aldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea
644691
Thermococcus litoralis
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus litoralis
?
an aldehyde + H2O + oxidized ferredoxin
-
644691
Thermococcus sp. ES1
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus sp. ES1
?
an aldehyde + H2O + oxidized ferredoxin
-
644691
Thermococcus sp. ES1
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus sp. ES1
ir
benzaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
benzoic acid + H+ + reduced benzyl viologen
-
-
-
ir
benzaldehyde + H2O + oxidized benzyl viologen
no activity
644691
Thermococcus litoralis
benzoic acid + H+ + reduced benzyl viologen
-
-
-
-
crotonaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
crotonaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
crotonaldehyde + H2O + oxidized benzyl viologen
no activity
644691
Thermococcus litoralis
pyruvate + H+ + reduced benzyl viologen
-
-
-
-
crotonaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp. ES1
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
formaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
formate + H+ + reduced benzyl viologen
-
644691
Thermococcus sp.
ir
formaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Pyrococcus furiosus
formate + H+ + reduced benzyl viologen
-
644691
Pyrococcus furiosus
ir
formaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
formate + H+ + reduced benzyl viologen
-
644691
Thermococcus litoralis
ir
formaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp. ES1
formate + H+ + reduced benzyl viologen
-
644691
Thermococcus sp. ES1
ir
glutaraldehyde + H2O + oxidized methyl viologen
-
644691
Pyrococcus furiosus
glutarate + H+ + reduced methyl viologen
-
-
-
-
glutaric dialdehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
?
-
-
-
ir
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
indoleacetate + H+ + reduced benzyl viologen
-
-
-
-
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
indoleacetate + H+ + reduced benzyl viologen
-
-
-
ir
indoleacetaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
indoleacetate + H+ + reduced benzyl viologen
-
-
-
ir
isovalerylaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
isovalerate + H+ + reduced benzyl viologen
-
-
-
ir
isovalerylaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase, no activity
644691
Pyrococcus furiosus
isovalerate + H+ + reduced benzyl viologen
-
-
-
-
isovalerylaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase, no activity
644691
Thermococcus litoralis
isovalerate + H+ + reduced benzyl viologen
-
-
-
-
phenylacetaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
phenylacetate + H+ + reduced benzyl viologen
-
-
-
ir
phenylacetaldehyde + H2O + oxidized benzyl viologen
no activity
644691
Thermococcus litoralis
phenylacetate + H+ + reduced benzyl viologen
-
-
-
-
phenylpropionaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
phenylpropanoate + H+ + reduced benzyl viologen
-
-
-
ir
propionaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
propionate + H+ + reduced benzyl viologen
-
644691
Thermococcus sp.
ir
propionaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Pyrococcus furiosus
propionate + H+ + reduced benzyl viologen
-
644691
Pyrococcus furiosus
ir
propionaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
propionate + H+ + reduced benzyl viologen
-
644691
Thermococcus litoralis
ir
succinic semialdehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
succinic acid + H+ + reduced benzyl viologen
-
-
-
ir
Subunits
Subunits
Commentary
Organism
dimer
; 2 * 67000
Pyrococcus furiosus
dimer
2 * 67000, SDS-PAGE
Thermococcus sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
80
-
assay at
Pyrococcus endeavori
80
-
assay at
Pyrococcus furiosus
80
-
assay at
Thermococcus litoralis
80
-
assay at
Thermococcus sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
80
-
aldehyde ferredoxin oxidoreductase, t1/2: 15 min; formaldehyde ferredoxin oxidoreductase, t1/2: 6 h
Pyrococcus furiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Pyrococcus endeavori
8.4
-
assay at
Pyrococcus furiosus
8.4
-
assay at
Thermococcus litoralis
8.4
-
assay at
Thermococcus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Pyrococcus endeavori
Ferredoxin
;
Pyrococcus furiosus
Ferredoxin
-
Thermococcus litoralis
Ferredoxin
-
Thermococcus sp.
tungsten cofactor
pterin ring system
Pyrococcus endeavori
tungsten cofactor
formaldehyde ferredoxin oxidoreductase; pterin ring system; pterin ring system
Pyrococcus furiosus
tungsten cofactor
formaldehyde ferredoxin oxidoreductase; pterin ring system
Thermococcus litoralis
tungsten cofactor
pterin ring system
Thermococcus sp.
tungsten-molybdopterin
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus endeavori
tungsten-molybdopterin
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus furiosus
tungsten-molybdopterin
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus litoralis
tungsten-molybdopterin
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Sulfide
4-5fold increase in activity at room temperature and pH 8.0 at 20 mM, in presence of 20 mM dithionite; activation of formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
Tungsten
stimulates cell growth
Pyrococcus furiosus
Tungsten
stimulates cell growth
Thermococcus litoralis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Pyrococcus endeavori
Ferredoxin
-
Pyrococcus furiosus
Ferredoxin
-
Thermococcus litoralis
Ferredoxin
-
Thermococcus sp.
tungsten cofactor
pterin ring system
Pyrococcus endeavori
tungsten cofactor
pterin ring system
Pyrococcus furiosus
tungsten cofactor
formaldehyde ferredoxin oxidoreductase; pterin ring system
Pyrococcus furiosus
tungsten cofactor
formaldehyde ferredoxin oxidoreductase; pterin ring system
Thermococcus litoralis
tungsten cofactor
pterin ring system
Thermococcus sp.
tungsten-molybdopterin
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus endeavori
tungsten-molybdopterin
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus furiosus
tungsten-molybdopterin
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus litoralis
tungsten-molybdopterin
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus sp.
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Pyrococcus furiosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
acetaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.028
-
isovalerylaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.05
-
Indoleacetaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.057
-
benzaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.076
-
phenylacetaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.14
-
crotonaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.15
-
propionaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
0.8
-
glutaric dialdehyde
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
Pyrococcus furiosus
1.42
-
formaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Thermococcus sp.
8
-
Succinic semialdehyde
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
Pyrococcus furiosus
15
-
phenylpropionaldehyde
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
Pyrococcus furiosus
25
-
formaldehyde
formaldehyde ferredoxin oxidoreductase, pH 8.4, 80°C, with benzyl viologen as electron acceptor
Pyrococcus furiosus
25
-
Indoleacetaldehyde
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
Pyrococcus furiosus
60
-
acetaldehyde
pH 8.4, 85°C, benzyl viologen as electron acceptor
Pyrococcus furiosus
60
-
propionaldehyde
pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor
Pyrococcus furiosus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 Ca2+ per subunit, formaldehyde ferredoxin oxidoreductase
Pyrococcus furiosus
Fe2+
contains 1 iron-sulfur cluster [4Fe-4S] per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus endeavori
Fe2+
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus furiosus
Fe2+
contains 1 additional Fe2+ besides the [4Fe-4S]-cluster; contains 1 iron-sulfur cluster [4Fe-4S] per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus furiosus
Fe2+
contains 1 iron-sulfur cluster [4Fe-4S] per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus litoralis
Fe2+
contains 1 iron-sulfur cluster [4Fe-4S] per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus sp.
Mg2+
both enzyme forms; contains 1 Mg-atom per subunit
Pyrococcus furiosus
Mg2+
contains 1 Mg-atom per subunit
Thermococcus sp.
Tungsten
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus endeavori
Tungsten
in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus furiosus
Tungsten
1 tungsten per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Pyrococcus furiosus
Tungsten
1 tungsten per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus litoralis
Tungsten
1 tungsten per subunit; in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues
Thermococcus sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
2 * 67000
Pyrococcus furiosus
67000
-
2 * 67000, SDS-PAGE
Thermococcus sp.
136100
-
-
Pyrococcus furiosus
280000
-
-
Pyrococcus furiosus
280000
-
-
Thermococcus litoralis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
an aldehyde + H2O + oxidized ferredoxin
Thermococcus sp.
-
an acid + H+ + reduced ferredoxin
-
Thermococcus sp.
?
an aldehyde + H2O + oxidized ferredoxin
Pyrococcus endeavori
-
an acid + H+ + reduced ferredoxin
-
Pyrococcus endeavori
?
an aldehyde + H2O + oxidized ferredoxin
Pyrococcus furiosus
formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea
an acid + H+ + reduced ferredoxin
-
Pyrococcus furiosus
?
an aldehyde + H2O + oxidized ferredoxin
Thermococcus litoralis
formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea
an acid + H+ + reduced ferredoxin
-
Thermococcus litoralis
?
an aldehyde + H2O + oxidized ferredoxin
Thermococcus sp. ES1
-
an acid + H+ + reduced ferredoxin
-
Thermococcus sp. ES1
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
aldehyde ferredoxin oxidoreductase, t1/2: 30 min at 23 °C
Pyrococcus furiosus
formaldehyde ferredoxin oxidoreductase, t1/2: 9 h at 23°C
Pyrococcus furiosus
Purification (Commentary) (protein specific)
Commentary
Organism
formaldehyde and aldehyde ferredoxin oxidoreductase; formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required
Pyrococcus furiosus
formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required
Thermococcus litoralis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
acetate + H+ + reduced benzyl viologen
-
644691
Thermococcus sp.
ir
acetaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Pyrococcus furiosus
acetate + H+ + reduced benzyl viologen
-
644691
Pyrococcus furiosus
ir
acetaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
acetate + H+ + reduced benzyl viologen
-
644691
Thermococcus litoralis
ir
acetaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp. ES1
acetate + H+ + reduced benzyl viologen
-
644691
Thermococcus sp. ES1
ir
an aldehyde + H2O + oxidized ferredoxin
-
644691
Pyrococcus furiosus
an acid + H+ + reduced ferredoxin
-
644691
Pyrococcus furiosus
ir
an aldehyde + H2O + oxidized ferredoxin
-
644691
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus sp.
?
an aldehyde + H2O + oxidized ferredoxin
-
644691
Thermococcus sp.
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus sp.
ir
an aldehyde + H2O + oxidized ferredoxin
-
644691
Thermococcus litoralis
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus litoralis
ir
an aldehyde + H2O + oxidized ferredoxin
-
644691
Pyrococcus endeavori
an acid + H+ + reduced ferredoxin
-
644691
Pyrococcus endeavori
?
an aldehyde + H2O + oxidized ferredoxin
-
644691
Pyrococcus endeavori
an acid + H+ + reduced ferredoxin
-
644691
Pyrococcus endeavori
ir
an aldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea
644691
Pyrococcus furiosus
an acid + H+ + reduced ferredoxin
-
644691
Pyrococcus furiosus
?
an aldehyde + H2O + oxidized ferredoxin
formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea
644691
Thermococcus litoralis
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus litoralis
?
an aldehyde + H2O + oxidized ferredoxin
-
644691
Thermococcus sp. ES1
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus sp. ES1
?
an aldehyde + H2O + oxidized ferredoxin
-
644691
Thermococcus sp. ES1
an acid + H+ + reduced ferredoxin
-
644691
Thermococcus sp. ES1
ir
benzaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
benzoic acid + H+ + reduced benzyl viologen
-
-
-
ir
benzaldehyde + H2O + oxidized benzyl viologen
no activity
644691
Thermococcus litoralis
benzoic acid + H+ + reduced benzyl viologen
-
-
-
-
crotonaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
crotonaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
crotonaldehyde + H2O + oxidized benzyl viologen
no activity
644691
Thermococcus litoralis
pyruvate + H+ + reduced benzyl viologen
-
-
-
-
crotonaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp. ES1
pyruvate + H+ + reduced benzyl viologen
-
-
-
ir
formaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
formate + H+ + reduced benzyl viologen
-
644691
Thermococcus sp.
ir
formaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Pyrococcus furiosus
formate + H+ + reduced benzyl viologen
-
644691
Pyrococcus furiosus
ir
formaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
formate + H+ + reduced benzyl viologen
-
644691
Thermococcus litoralis
ir
formaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp. ES1
formate + H+ + reduced benzyl viologen
-
644691
Thermococcus sp. ES1
ir
glutaraldehyde + H2O + oxidized methyl viologen
-
644691
Pyrococcus furiosus
glutarate + H+ + reduced methyl viologen
-
-
-
-
glutaric dialdehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
?
-
-
-
ir
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
644691
Pyrococcus furiosus
indoleacetate + H+ + reduced benzyl viologen
-
-
-
-
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
indoleacetate + H+ + reduced benzyl viologen
-
-
-
ir
indoleacetaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
indoleacetate + H+ + reduced benzyl viologen
-
-
-
ir
isovalerylaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
isovalerate + H+ + reduced benzyl viologen
-
-
-
ir
isovalerylaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase, no activity
644691
Pyrococcus furiosus
isovalerate + H+ + reduced benzyl viologen
-
-
-
-
isovalerylaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase, no activity
644691
Thermococcus litoralis
isovalerate + H+ + reduced benzyl viologen
-
-
-
-
phenylacetaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
phenylacetate + H+ + reduced benzyl viologen
-
-
-
ir
phenylacetaldehyde + H2O + oxidized benzyl viologen
no activity
644691
Thermococcus litoralis
phenylacetate + H+ + reduced benzyl viologen
-
-
-
-
phenylpropionaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
phenylpropanoate + H+ + reduced benzyl viologen
-
-
-
ir
propionaldehyde + H2O + oxidized benzyl viologen
-
644691
Thermococcus sp.
propionate + H+ + reduced benzyl viologen
-
644691
Thermococcus sp.
ir
propionaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Pyrococcus furiosus
propionate + H+ + reduced benzyl viologen
-
644691
Pyrococcus furiosus
ir
propionaldehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
propionate + H+ + reduced benzyl viologen
-
644691
Thermococcus litoralis
ir
succinic semialdehyde + H2O + oxidized benzyl viologen
formaldehyde ferredoxin oxidoreductase
644691
Thermococcus litoralis
succinic acid + H+ + reduced benzyl viologen
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
-
Pyrococcus furiosus
dimer
2 * 67000
Pyrococcus furiosus
dimer
2 * 67000, SDS-PAGE
Thermococcus sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
80
-
assay at
Pyrococcus endeavori
80
-
assay at
Pyrococcus furiosus
80
-
assay at
Thermococcus litoralis
80
-
assay at
Thermococcus sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
80
-
aldehyde ferredoxin oxidoreductase, t1/2: 15 min; formaldehyde ferredoxin oxidoreductase, t1/2: 6 h
Pyrococcus furiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Pyrococcus endeavori
8.4
-
assay at
Pyrococcus furiosus
8.4
-
assay at
Thermococcus litoralis
8.4
-
assay at
Thermococcus sp.
Other publictions for EC 1.2.7.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740953
Liew
Metabolic engineering of Clost ...
Clostridium autoethanogenum
Metab. Eng.
40
104-114
2017
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
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-
-
-
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1
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-
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-
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1
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
740209
Ammam
Effect of tungstate on acetate ...
Sporomusa ovata, Sporomusa ovata DSM-2662
Biotechnol. Biofuels
9
163
2016
-
-
-
-
-
-
-
-
-
1
-
2
-
4
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-
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2
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1
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2
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737164
Basen
Single gene insertion drives b ...
Pyrococcus furiosus
Proc. Natl. Acad. Sci. USA
111
17618-17623
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740626
Debnar-Daumler
Simultaneous involvement of a ...
Aromatoleum aromaticum
J. Bacteriol.
196
483-492
2014
-
-
-
-
-
-
-
-
-
2
1
1
-
3
-
-
1
-
-
-
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
2
1
1
-
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-
1
-
-
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722823
Liao
Why is the molybdenum-substitu ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
18
175-181
2013
-
-
-
-
-
-
-
-
-
2
-
1
-
1
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1
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-
2
-
1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722893
Liao
Tungsten-dependent formaldehyd ...
Pyrococcus furiosus
J. Inorg. Biochem.
105
927-936
2011
-
-
-
-
-
-
-
-
-
1
-
1
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
745210
Sevcenco
Molybdenum incorporation in t ...
Pyrococcus furiosus
J. Bacteriol.
192
4143-4152
2010
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
1
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-
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-
-
1
-
-
-
-
-
-
-
-
1
1
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-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
692610
Sugimoto
In situ generation of oxo-sulf ...
synthetic construct
Inorg. Chem.
46
8460-8462
2007
-
1
-
-
-
-
-
-
-
-
-
-
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1
-
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1
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1
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1
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-
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-
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-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
675237
Thapper
Biochemical and spectroscopic ...
Desulfovibrio aminophilus, Desulfovibrio aminophilus DSM 12254
J. Inorg. Biochem.
100
44-50
2006
-
-
-
-
-
-
-
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2
2
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2
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1
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5
1
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-
2
2
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1
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656293
Hagedoorn
Purification and characterizat ...
Pyrobaculum aerophilum
J. Biol. Inorg. Chem.
10
259-269
2005
-
-
-
-
-
-
-
1
-
3
2
-
-
4
-
-
1
-
-
-
1
-
6
1
1
-
-
-
-
-
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-
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-
-
-
-
-
-
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1
-
3
2
-
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-
-
1
-
-
1
-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
674251
Bevers
WOR5, a novel tungsten-contain ...
Pyrococcus furiosus
J. Bacteriol.
187
7056-7061
2005
-
-
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1
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4
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4
-
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-
-
1
1
-
-
-
654430
Wahyudi
Characterization of aldehyde f ...
Magnetospirillum magneticum, Magnetospirillum magneticum AMB-1
Biochem. Biophys. Res. Commun.
303
223-229
2003
-
-
-
-
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1
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2
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8
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1
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2
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1
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1
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2
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-
-
-
644691
Roy
Aldehyde oxidoreductases from ...
Pyrococcus endeavori, Pyrococcus furiosus, Thermococcus litoralis, Thermococcus sp., Thermococcus sp. ES1
Methods Enzymol.
331
132-144
2001
3
-
-
1
-
-
-
15
-
11
5
5
-
7
2
-
2
-
-
-
-
-
40
2
4
-
1
-
4
-
-
12
-
-
-
3
-
-
15
1
-
-
-
-
-
15
-
13
5
5
-
2
-
2
-
-
-
-
40
3
4
-
1
-
4
-
-
-
-
-
-
-
-
-
745202
Adams
Key role for sulfur in peptid ...
Pyrococcus furiosus
J. Bacteriol.
183
716-724
2001
-
-
-
-
-
-
-
-
-
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1
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1
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1
-
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-
-
-
-
-
1
-
-
1
-
-
644689
Roy
Purification and molecular cha ...
Pyrococcus furiosus
J. Bacteriol.
181
1171-1180
1999
1
-
-
-
-
1
-
2
-
4
2
1
-
4
-
-
1
-
-
-
2
-
12
1
1
1
2
-
1
1
-
2
-
-
-
1
-
-
2
-
-
1
-
-
-
2
-
4
2
1
-
-
-
1
-
-
2
-
12
1
1
1
2
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1
1
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644690
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Formaldehyde ferredoxin oxidor ...
Pyrococcus furiosus
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1999
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1
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721578
Zhou
Site-directed mutations of the ...
Pyrococcus furiosus
Biochemistry
36
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1997
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4
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1
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1
644687
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Spectroscopic characterization ...
Pyrococcus endeavori, Pyrococcus furiosus
J. Am. Chem. Soc.
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12391-12405
1996
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6
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6
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2
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644688
Das
-
Modeling the tungsten sites of ...
Pyrococcus furiosus
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1387-1397
1996
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2
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722057
Arendsen
-
Redox chemistry of biological ...
Pyrococcus furiosus
Chemistry
1
292-296
1996
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2
2
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1
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722490
Mukund
Molybdenum and vanadium do not ...
Pyrococcus furiosus
J. Bacteriol.
178
163-167
1996
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1
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11935
Heider
Purification, characterization ...
Thermococcus sp., Thermococcus sp. ES1
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4757-4764
1995
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15
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2
1
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8
1
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16
1
1
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2
15
1
1
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4
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4
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11
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15
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6
2
1
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1
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16
1
1
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2
15
1
1
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644684
Kletzin
Molecular characterization of ...
Pyrococcus furiosus
J. Bacteriol.
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4817-4819
1995
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4
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644686
Chan
Structure of a hyperthermophil ...
Pyrococcus furiosus
Science
267
1463-1469
1995
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3
2
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2
1
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722438
George
-
Aldehyde ferredoxin oxidoreduc ...
Pyrococcus furiosus
J. Am. Chem. Soc.
114
3521-3523
1992
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11938
Mukund
The novel tungsten-iron-sulfur ...
Pyrococcus furiosus
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266
14208-14216
1991
1
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5
2
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2
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1
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15
1
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5
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2
2
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15
1
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