Crystallization (Comment) | Organism |
---|---|
formaldehyde ferredoxin oxidoreductase, purified enzyme, modified melting-point capillary method, room temperature under argon atmosphere, protein solution: 55-65 mg/ml, 50 mM Tris-HCl, pH 8.0, 2 mM dithionite, 2 mM DTT, 0.2 M KCl, precipitant solution: 30% v/v glycerol, 20% w/v PEG 4000, 0.1 M sodium citrate, pH 5.6, 0.2 M NaCl, several weeks, X-ray structure determination and analysis | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | binding site, formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | |
Fe2+ | contains 1 iron-sulfur cluster [4Fe-4S] per subunit | Pyrococcus furiosus | |
Fe2+ | the [4Fe-4S]-cluster is coordinated by Cys287 of the formaldehyde ferredoxin oxidoreductase and Asp14 of ferredoxin, structure | Pyrococcus furiosus | |
Tungsten | - |
Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
hyperthermophilic archaeon | - |
Pyrococcus furiosus | - |
Protein Data Bank entries 1B25 and 1B4N | - |
Pyrococcus furiosus | - |
contains a formaldehyde and an aldehyde ferredoxin oxidoreductase | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin | active site structure | Pyrococcus furiosus | |
an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin | intermolecular interaction analysis | Pyrococcus furiosus | |
an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin | substrate binding, catalytic site and reaction mechanism including cofactor action | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aldehyde + H2O + oxidized ferredoxin | formaldehyde ferredoxin oxidoreductase, C1-C3 aldehydes | Pyrococcus furiosus | an acid + H+ + reduced ferredoxin | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
More | formaldehyde ferredoxin oxidoreductase and aldehyde ferredoxin oxidoreductase are 2 separate enzymes | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | formaldehyde ferredoxin oxidoreductase, binding mechanism | Pyrococcus furiosus | |
tungsten cofactor | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | |
tungsten-molybdopterin | tungstopterin center | Pyrococcus furiosus |