Protein Variants | Comment | Organism |
---|---|---|
C338A | complete loss of activity. Unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ | Rhodospirillum rubrum |
C451A | complete loss of activity. Unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ | Rhodospirillum rubrum |
C451S | 1.4% of wild-type specific activity. Unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ | Rhodospirillum rubrum |
C481A | unable to grow in the dark with CO as the energy source, amount of enzyme present in membrane fraction is similar to wild-type as well as accumulation of Ni2+ | Rhodospirillum rubrum |
C531A | 0.1% of wild-type specific activity | Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
CO | mutant C451S, pH 7.5 | Rhodospirillum rubrum | |
0.032 | - |
CO | wild-type, pH 7.5 | Rhodospirillum rubrum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rhodospirillum rubrum | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | wild-type, 8.97 mol per mol of enzyme | Rhodospirillum rubrum | |
Nickel | wild-type, 0.85 mol per mol of enzyme. Analysis of activation of wild-type and mutant apo-enzymes by nickel | Rhodospirillum rubrum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.9 | - |
mutant C531A, pH 7.5 | Rhodospirillum rubrum |
86.3 | - |
mutant C451S, pH 7.5 | Rhodospirillum rubrum |
6230 | - |
wild-type, pH 7.5 | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + methyl viologen | - |
Rhodospirillum rubrum | CO2 + reduced methyl viologen | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
mutant C451S | Rhodospirillum rubrum |
8.6 | - |
wild-type | Rhodospirillum rubrum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | wild-type and mutants apo-enzymes, stable for at least 30 min within this range | Rhodospirillum rubrum |