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Literature summary for 1.2.7.4 extracted from

  • Drake, H.L.; Hu, S.I.; Wood, H.G.
    Purification of carbon monoxide dehydrogenase, a nickel enzyme from Clostridium thermoaceticum (1980), J. Biol. Chem., 255, 7174-7180.
    View publication on PubMed
Search for more literature for 1.2.7.4

Inhibitors

Inhibitors Comment Organism Structure
CN- KCN, reversible Moorella thermoacetica
KCN CO reverses cyanide inhibition, but promotes reaction with methyl iodide Moorella thermoacetica
additional information not affected by propyl iodide, methyl iodide, carbon tetrachloride,or metal chelators; not inhibited by propyl iodide, methyl iodide, carbon tetrachloride, EDTA or nitrilotriacetate Moorella thermoacetica

Metals/Ions

Metals/Ions Comment Organism Structure
Ni2+
-
 Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
410000
-
 gel filtration Moorella thermoacetica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CO + H2O + electron acceptor Moorella thermoacetica ferredoxin and a membrane-bound b-type cytochrome are considered to be the native electron carriers ?
-
 ?
CO + H2O + oxidized cytochrome b Moorella thermoacetica membrane-bound b-type, native electron carrier CO2 + reduced cytochrome b
-
 ?
CO + H2O + oxidized ferredoxin Moorella thermoacetica
-
 CO2 + reduced ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
-
-
Moorella thermoacetica
-
 formerly Clostridium thermoaceticum
-

Oxidation Stability

Oxidation Stability Organism
enzymatic activity is destroyed by air, a low amount of activity still is present after 2 h exposure to air Moorella thermoacetica

Purification (Commentary)

Purification (Comment) Organism
-
 Moorella thermoacetica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 method for in situ assay Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + cytochrome b
-
 Moorella thermoacetica CO2 + reduced cytochrome b
-
 ?
CO + H2O + cytochrome c3 cytochrome 3 from Desulfovibrio vulgaris Moorella thermoacetica CO2 + reduced cytochrome c3
-
 ?
CO + H2O + electron acceptor ferredoxin and a membrane-bound b-type cytochrome are considered to be the native electron carriers Moorella thermoacetica ?
-
 ?
CO + H2O + ferredoxin active with ferredoxin obtained from Clostridium thermoaceticum, no activity with ferredoxin from spinach Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
CO + H2O + FMN
-
 Moorella thermoacetica CO2 + FMNH2
-
 ?
CO + H2O + methyl viologen no activity with FAD, NAD+ and NADP+ Moorella thermoacetica CO2 + reduced methyl viologen
-
 ?
CO + H2O + oxidized cytochrome b membrane-bound b-type, native electron carrier Moorella thermoacetica CO2 + reduced cytochrome b
-
 ?
CO + H2O + oxidized cytochrome c3 Desulfovibrio vulgaris cytochrome c3 Moorella thermoacetica CO2 + reduced cytochrome c3
-
 ?
CO + H2O + oxidized ferredoxin
-
 Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
CO + H2O + oxidized ferredoxin ferredoxin from Clostridium pasteurianum is also a substrate Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
CO + H2O + oxidized flavodoxin
-
 Moorella thermoacetica CO2 + reduced flavodoxin
-
 ?
CO + H2O + oxidized methyl viologen
-
 Moorella thermoacetica CO2 + reduced methyl viologen
-
 ?
additional information spinach ferredoxin, FAD, NAD+ and NADP+ are not reduced Moorella thermoacetica ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Moorella thermoacetica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.6
-
-
 Moorella thermoacetica
10.6
-
 metyl viologen as substrate Moorella thermoacetica