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Literature summary for 1.2.7.3 extracted from
- A reverse TCA cycle 2-oxoacid ferredoxin oxidoreductase that makes C-C bonds from CO2 (2019), Joule, 3, 595-611 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Magnetococcus marinus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structure of both native and substrate-bound forms, to 1.94 and 2.8 A resolution, respectively | Magnetococcus marinus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E45Q | mutation in subunit alpha, residue forms second coordination sphere interactions with Arg63 of subunit beta. Mutant has a lower KM for 2-oxoglutarate than wild-type while kcat is diminished | Magnetococcus marinus |
| Y436F | mutation in subunit alpha, residue forms second coordination sphere interactions with Arg63 of subunit beta. Mutant has a lower KM for 2-oxoglutarate than wild-type while kcat is diminished | Magnetococcus marinus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.64 | - |
2-oxoglutarate | mutant E45Q, pH 7.5, temperature not specified in the publication | Magnetococcus marinus |  |
| 0.97 | - |
2-oxoglutarate | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 1.3 | - |
reduced ferredoxin | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 1.8 | - |
oxidized ferredoxin | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 1.8 | - |
2-oxoglutarate | mutant Y436F, pH 7.5, temperature not specified in the publication | Magnetococcus marinus | |
| 4.4 | - |
2-oxoglutarate | wild-type, pH 8.5, temperature not specified in the publication | Magnetococcus marinus | |
| 4.9 | - |
2-oxoglutarate | wild-type, pH 7.5, temperature not specified in the publication | Magnetococcus marinus | |
| 6.8 | - |
CoA | wild-type, pH 8.5, temperature not specified in the publication | Magnetococcus marinus | |
| 10 | - |
CoA | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 32 | - |
succinyl-CoA | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Magnetococcus marinus | A0L8G4 and A0L8G5 | A0L8G4 i.e. pyruvate flavodoxin/ferredoxin oxidoreductase domain protein, A0L8G5 i.e. beta subunit | - |
| Magnetococcus marinus MC-1 | A0L8G4 and A0L8G5 | A0L8G4 i.e. pyruvate flavodoxin/ferredoxin oxidoreductase domain protein, A0L8G5 i.e. beta subunit | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + CoA + 2 oxidized ferredoxin | - |
Magnetococcus marinus | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
r | |
| 2-oxoglutarate + CoA + 2 oxidized ferredoxin | - |
Magnetococcus marinus MC-1 | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
r | |
| additional information | enzyme is both CO2-fixing and 2-oxoglutarate oxidizing. Negligible activity with other 2-oxoacids, including glyoxylate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, 3-methyl-2-oxovalerate, phenylpyruvate and oxaloacetate and 2-oxoadipate | Magnetococcus marinus | ? | - |
- |
|
| additional information | enzyme is both CO2-fixing and 2-oxoglutarate oxidizing. Negligible activity with other 2-oxoacids, including glyoxylate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, 3-methyl-2-oxovalerate, phenylpyruvate and oxaloacetate and 2-oxoadipate | Magnetococcus marinus MC-1 | ? | - |
- |
|
| succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
Magnetococcus marinus | 2-oxoglutarate + CoA + 2 oxidized ferredoxin | - |
? | |
| succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
Magnetococcus marinus MC-1 | 2-oxoglutarate + CoA + 2 oxidized ferredoxin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mmc1_1749 | gene name, pyruvate flavodoxin/ferredoxin oxidoreductase domain protein | Magnetococcus marinus |
| Mmc1_1750 | gene name, beta subunit | Magnetococcus marinus |
| OGOR | - |
Magnetococcus marinus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
succinyl-CoA | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 0.51 | - |
reduced ferredoxin | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 6.8 | - |
2-oxoglutarate | mutant E45Q, pH 7.5, temperature not specified in the publication | Magnetococcus marinus | |
| 10.8 | - |
2-oxoglutarate | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 11.2 | - |
CoA | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 12.3 | - |
oxidized ferredoxin | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 17.8 | - |
2-oxoglutarate | mutant Y436F, pH 7.5, temperature not specified in the publication | Magnetococcus marinus | |
| 22.2 | - |
2-oxoglutarate | wild-type, pH 7.5, temperature not specified in the publication | Magnetococcus marinus | |
| 24.7 | - |
CoA | wild-type, pH 8.5, temperature not specified in the publication | Magnetococcus marinus | |
| 30.3 | - |
2-oxoglutarate | wild-type, pH 8.5, temperature not specified in the publication | Magnetococcus marinus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 10.5 | - |
Magnetococcus marinus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| [4Fe-4S]-center | protein contains 3.6 mol of iron and 3.4 mol of sulfur per mol of alphabeta heterodimer | Magnetococcus marinus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | OGOR operates bidirectionally, both CO2-fixing and 2-oxoglutarate oxidizing, and only ferredoxin Fd1 supports efficient catalysis. Residue Glu45alpha is a mobile residue that impacts catalytic bias toward CO2-fixation although it makes no direct contact with TPP-bound intermediates | Magnetococcus marinus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
2-oxoglutarate | wild-type, pH 7.5, temperature not specified in the publication | Magnetococcus marinus | |
| 6.9 | - |
2-oxoglutarate | wild-type, pH 8.5, temperature not specified in the publication | Magnetococcus marinus | |
| 9.9 | - |
2-oxoglutarate | mutant Y436F, pH 7.5, temperature not specified in the publication | Magnetococcus marinus | |
| 11 | - |
2-oxoglutarate | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 11 | - |
2-oxoglutarate | mutant E45Q, pH 7.5, temperature not specified in the publication | Magnetococcus marinus | |
| 14 | - |
succinyl-CoA | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 390 | - |
reduced ferredoxin | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 1100 | - |
CoA | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
| 3600 | - |
CoA | wild-type, pH 8.5, temperature not specified in the publication | Magnetococcus marinus | |
| 6800 | - |
oxidized ferredoxin | wild-type, pH 7.0, temperature not specified in the publication | Magnetococcus marinus | |
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