BRENDA - Enzyme Database show
show all sequences of 1.2.7.12

Carbon-dependent control of electron transfer and central carbon pathway genes for methane biosynthesis in the Archaean, Methanosarcina acetivorans strain C2A

Rohlin, L.; Gunsalus, R.P.; BMC Microbiol. 10, 62 (2010)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formylmethanofuran + H2O + oxidized ferredoxin
Methanosarcina acetivorans
-
CO2 + methanofuran + reduced ferredoxin
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanosarcina acetivorans
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formylmethanofuran + H2O + oxidized ferredoxin
-
711603
Methanosarcina acetivorans
CO2 + methanofuran + reduced ferredoxin
-
-
-
r
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formylmethanofuran + H2O + oxidized ferredoxin
Methanosarcina acetivorans
-
CO2 + methanofuran + reduced ferredoxin
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formylmethanofuran + H2O + oxidized ferredoxin
-
711603
Methanosarcina acetivorans
CO2 + methanofuran + reduced ferredoxin
-
-
-
r
Expression
Organism
Commentary
Expression
Methanosarcina acetivorans
the molybdenum-type formylmethanofuran dehydrogenase operon reporter genes fmdE1 and fmdA1 are expressed at 14fold higher levels during methanol growth conditions relative to acetate growth. Genes fmdF2, fmdA2, and fmdB2 are expressed about 2fold higher during these conditions, but the maximal level of expression is less than 5% of that seen for the fmdE1 and fmdA1 genes. The tungsten-type formylmethanofuran dehydrogenase genes fwdB1 and fwdA1 are also expressed 15fold higher levels during methanol growth relative to acetate
up
Expression (protein specific)
Organism
Commentary
Expression
Methanosarcina acetivorans
the molybdenum-type formylmethanofuran dehydrogenase operon reporter genes fmdE1 and fmdA1 are expressed at 14fold higher levels during methanol growth conditions relative to acetate growth. Genes fmdF2, fmdA2, and fmdB2 are expressed about 2fold higher during these conditions, but the maximal level of expression is less than 5% of that seen for the fmdE1 and fmdA1 genes. The tungsten-type formylmethanofuran dehydrogenase genes fwdB1 and fwdA1 are also expressed 15fold higher levels during methanol growth relative to acetate
up
Other publictions for EC 1.2.7.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739961
Matschiavelli
Role of a putative tungsten-de ...
Methanosarcina acetivorans
Arch. Microbiol.
197
379-388
2015
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
726376
Kaster
Coupling of ferredoxin and het ...
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
Proc. Natl. Acad. Sci. USA
108
2981-2986
2011
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710739
Axelrod
Structures of three members of ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense DCB-2, Syntrophus aciditrophicus, Syntrophus aciditrophicus SB, Thermoplasma acidophilum
Acta Crystallogr. Sect. F
66
1335-1346
2010
-
-
3
3
-
-
-
-
-
4
3
-
-
12
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
3
-
3
-
-
-
-
-
-
-
4
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
711603
Rohlin
Carbon-dependent control of el ...
Methanosarcina acetivorans
BMC Microbiol.
10
62
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
713413
Costa
Protein complexing in a methan ...
Methanothermobacter marburgensis, Methanothermobacter marburgensis S2
Proc. Natl. Acad. Sci. USA
107
11050-11055
2010
-
-
-
-
-
-
-
-
-
1
-
2
-
5
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690436
Andreesen
Tungsten, the surprisingly pos ...
Methanothermobacter wolfeii
Ann. N. Y. Acad. Sci.
1125
215-229
2008
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655637
Stojanowic
CO2 reduction to the level of ...
Methanosarcina barkeri
FEMS Microbiol. Lett.
235
163-167
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655471
de Poorter
Bioenergetics of the formyl-me ...
Methanothermobacter thermautotrophicus
Eur. J. Biochem.
270
66-75
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741352
Meuer
Genetic analysis of the archae ...
Methanosarcina barkeri
Proc. Natl. Acad. Sci. USA
99
5632-5637
2002
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11928
Hochheimer
The formylmethanofuran dehydro ...
Methanothermobacter thermautotrophicus, Methanothermobacter wolfeii
Arch. Microbiol.
170
389-393
1998
-
-
-
-
-
-
-
-
-
4
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11931
Vorholt
A selenium-dependent and a sel ...
Methanopyrus kandleri
Mol. Microbiol.
23
1033-1042
1997
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11933
Vorholt
The active species of 'CO2' ut ...
Methanosarcina barkeri, Methanothermobacter thermautotrophicus
Eur. J. Biochem.
248
919-924
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721443
Vorholt
Pathways of autotrophic CO2 fi ...
Ferroglobus placidus
Arch. Microbiol.
167
19-23
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
11919
Vorholt
A polyferredoxin with eight [4 ...
Methanosarcina barkeri
Eur. J. Biochem.
236
309-317
1996
-
-
1
-
-
-
-
1
-
2
4
1
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
4
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11934
Hochheimer
The molybdenum formylmethanofu ...
Methanothermobacter thermautotrophicus
Eur. J. Biochem.
242
156-162
1996
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721441
Vornolt
-
Enzymes and coenzymes of the c ...
Archaeoglobus fulgidus, Archaeoglobus lithotrophicus, Archaeoglobus lithotrophicus TF-2, Archaeoglobus profundus, Archaeoglobus profundus DSM 5631
Arch. Microbiol.
163
112-118
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
3
3
-
-
-
3
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
3
-
-
-
3
-
-
-
-
3
3
-
-
-
11920
Bertram
Thermodynamics of the formylme ...
Methanothermobacter thermautotrophicus
Eur. J. Biochem.
226
811-818
1994
-
-
-
-
-
-
-
7
-
-
-
1
-
4
-
-
-
-
-
-
-
-
6
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
1
-
-
-
-
-
-
-
-
6
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
11921
Bertram
Formylmethanofuran dehydrogena ...
Methanosarcina barkeri, Methanothermobacter thermautotrophicus, Methanothermobacter wolfeii
Eur. J. Biochem.
220
477-484
1994
-
-
-
-
-
-
3
10
-
5
-
-
-
3
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
10
-
5
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11929
Bertram
Tungstate can substitute for m ...
Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus Marburg / DSM 2133
Arch. Microbiol.
161
220-228
1994
-
-
-
-
-
-
-
2
-
2
5
-
-
33
-
-
1
-
-
-
1
-
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
5
-
-
-
-
1
-
-
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
11922
Schmitz
A molybdenum and a tungsten is ...
Methanothermobacter wolfeii
Eur. J. Biochem.
209
1013-1018
1992
-
-
-
-
-
-
1
4
-
3
5
-
-
4
-
-
1
-
-
-
1
-
3
2
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
5
-
-
-
-
1
-
-
1
-
3
2
-
-
2
-
1
1
-
-
-
-
-
-
-
-
11926
Schmitz
Properties of the tungsten-sub ...
Methanothermobacter wolfeii
FEBS Lett.
309
78-81
1992
-
-
-
-
-
-
-
5
-
2
-
-
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
11927
Brner
Molybdopterin adenine dinucleo ...
Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus Marburg / DSM 2133
FEBS Lett.
290
31-34
1991
-
-
-
-
-
-
-
2
-
3
1
-
-
32
-
-
1
-
-
-
1
-
4
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
3
1
-
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
11930
Schmitz
-
N5,N10-Methylenetetrahydrometh ...
Archaeoglobus fulgidus
Arch. Microbiol.
156
427-434
1991
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11923
Karrasch
The molybdoenzyme formylmethan ...
Methanosarcina barkeri
Eur. J. Biochem.
194
367-372
1990
-
-
-
-
-
1
-
1
-
2
9
-
-
4
-
-
1
-
-
-
-
-
1
1
1
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
2
9
-
-
-
-
1
-
-
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
11925
Breitung
N-Furfurylformamide as a pseud ...
Methanosarcina barkeri, Methanothermobacter thermautotrophicus
FEBS Lett.
268
257-260
1990
-
-
-
-
-
-
-
5
-
-
-
-
-
5
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11932
Karrasch
The molybdenum cofactor of for ...
Methanosarcina barkeri
FEBS Lett.
274
48-52
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11924
Karrasch
Formylmethanofuran dehydrogena ...
Methanosarcina barkeri, Methanothermobacter thermautotrophicus
FEBS Lett.
253
226-230
1989
-
-
-
-
-
-
-
-
-
2
-
2
-
5
-
-
1
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-