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Literature summary for 1.2.7.12 extracted from
- Structures of three members of Pfam PF02663 (FmdE) implicated in microbial methanogenesis reveal a conserved alpha+beta core domain and an auxiliary C-terminal treble-clef zinc finger (2010), Acta Crystallogr. Sect. F, 66, 1335-1346.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Thermoplasma acidophilum |
| expressed in Escherichia coli | Desulfitobacterium hafniense |
| expressed in Escherichia coli | Syntrophus aciditrophicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| nanodroplet vapor diffusion method, using 0.01 M nickel chloride, 20% (w/v) PEG MME 2000 and 0.1 M Tris pH 8.5 | Syntrophus aciditrophicus |
| nanodroplet vapor diffusion method, using 0.2 M magnesium nitrate and 20% (w/v) PEG 3350 or 10% (w/v) PEG 8000, 0.2 M zinc acetate and 0.1 M MES pH 6.0 | Thermoplasma acidophilum |
| nanodroplet vapor diffusion method, using 0.2 M MgCl2 and 20% (w/v) PEG 3350 | Desulfitobacterium hafniense |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ni2+ | contains nickel | Desulfitobacterium hafniense |  |
| Zn2+ | contains zinc | Thermoplasma acidophilum | |
| Zn2+ | contains zinc | Desulfitobacterium hafniense | |
| Zn2+ | contains zinc | Syntrophus aciditrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17400 | - |
calculated from amino acid sequence | Desulfitobacterium hafniense |
| 21500 | - |
calculated from amino acid sequence | Syntrophus aciditrophicus |
| 23100 | - |
calculated from amino acid sequence | Thermoplasma acidophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfitobacterium hafniense | B8FYU2 | subunit E | - |
| Desulfitobacterium hafniense DCB-2 | B8FYU2 | subunit E | - |
| Syntrophus aciditrophicus | Q2LQ23 | subunit E | - |
| Syntrophus aciditrophicus SB | Q2LQ23 | subunit E | - |
| Thermoplasma acidophilum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-chelating resin column chromatography | Thermoplasma acidophilum |
| nickel-chelating resin column chromatography | Desulfitobacterium hafniense |
| nickel-chelating resin column chromatography | Syntrophus aciditrophicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FmdE | - |
Thermoplasma acidophilum |
| FmdE | - |
Desulfitobacterium hafniense |
| FmdE | - |
Syntrophus aciditrophicus |
| PF02663 | - |
Thermoplasma acidophilum |
| PF02663 | - |
Desulfitobacterium hafniense |
| PF02663 | - |
Syntrophus aciditrophicus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Desulfitobacterium hafniense | calculated from amino acid sequence | - |
5.6 |
| Thermoplasma acidophilum | calculated from amino acid sequence | - |
6.1 |
| Syntrophus aciditrophicus | calculated from amino acid sequence | - |
6.2 |
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