BRENDA - Enzyme Database show
show all sequences of 1.2.7.11

2-Oxoacid:ferredoxin oxidoreductase from the thermoacidophilic archaeon, Sulfolobus sp. strain 7

Zhang, Q.; Iwaaki, T.; Wakagi, T.; Oshima, T.; J. Biochem. 120, 587-599 (1996)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Sulfolobus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
pyruvate
pH 6.8, 50C
Sulfolobus sp.
0.87
-
2-oxoglutarate
pH 6.8, 50C
Sulfolobus sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
contains one [4Fe-4S]2+,1+ cluster per alpha/beta structure
Sulfolobus sp.
Mg2+
contains 1.4 mol of Mg per mol of protein
Sulfolobus sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sulfolobus sp.
P72578 and P72579
P72578: subunit alpha, P72579: subunit beta
-
Sulfolobus sp. 7
P72578 and P72579
P72578: subunit alpha, P72579: subunit beta
-
Oxidation Stability
Oxidation Stability
Organism
purified enzyme is stable against oxygen
Sulfolobus sp.
Purification (Commentary)
Commentary
Organism
-
Sulfolobus sp.
Storage Stability
Storage Stability
Organism
-80C, purified enzyme can be stored after dialysis against 20 mM potassium phosphate buffer, pH 6.8
Sulfolobus sp.
20C, 4C or -80C, activity in cytosolic fraction is not very stable
Sulfolobus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxobutyrate + CoA + oxidized ferredoxin
-
288435
Sulfolobus sp.
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
2-oxobutyrate + CoA + oxidized ferredoxin
-
288435
Sulfolobus sp. 7
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
288435
Sulfolobus sp.
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
288435
Sulfolobus sp. 7
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
pyruvate + CoA + 2 oxidized ferredoxin
Vmax/Km is 1.9fold higher than that for 2-oxoglutarate
288435
Sulfolobus sp.
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
pyruvate + CoA + 2 oxidized ferredoxin
Vmax/Km is 1.9fold higher than that for 2-oxoglutarate
288435
Sulfolobus sp. 7
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
assay at
Sulfolobus sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
assay at
Sulfolobus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
the enzyme contains one thiamine diphosphate per alphabeta structure
Sulfolobus sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Sulfolobus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
the enzyme contains one thiamine diphosphate per alphabeta structure
Sulfolobus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
pyruvate
pH 6.8, 50C
Sulfolobus sp.
0.87
-
2-oxoglutarate
pH 6.8, 50C
Sulfolobus sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
contains one [4Fe-4S]2+,1+ cluster per alpha/beta structure
Sulfolobus sp.
Mg2+
contains 1.4 mol of Mg per mol of protein
Sulfolobus sp.
Oxidation Stability (protein specific)
Oxidation Stability
Organism
purified enzyme is stable against oxygen
Sulfolobus sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sulfolobus sp.
Storage Stability (protein specific)
Storage Stability
Organism
-80C, purified enzyme can be stored after dialysis against 20 mM potassium phosphate buffer, pH 6.8
Sulfolobus sp.
20C, 4C or -80C, activity in cytosolic fraction is not very stable
Sulfolobus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxobutyrate + CoA + oxidized ferredoxin
-
288435
Sulfolobus sp.
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
2-oxobutyrate + CoA + oxidized ferredoxin
-
288435
Sulfolobus sp. 7
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
288435
Sulfolobus sp.
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
288435
Sulfolobus sp. 7
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
pyruvate + CoA + 2 oxidized ferredoxin
Vmax/Km is 1.9fold higher than that for 2-oxoglutarate
288435
Sulfolobus sp.
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
pyruvate + CoA + 2 oxidized ferredoxin
Vmax/Km is 1.9fold higher than that for 2-oxoglutarate
288435
Sulfolobus sp. 7
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
assay at
Sulfolobus sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
assay at
Sulfolobus sp.
Other publictions for EC 1.2.7.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739701
Yan
Crystal structures of archaeal ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7, Sulfurisphaera tokodaii DSM 16993
Sci. Rep.
6
33061
2016
-
-
1
1
4
-
-
9
-
1
2
4
-
11
-
-
1
-
-
-
-
-
32
1
1
-
-
-
1
-
-
3
-
-
-
-
-
4
10
4
8
-
-
-
-
18
-
2
4
4
-
-
-
4
-
-
-
-
32
4
2
-
-
-
2
-
-
-
-
1
2
-
-
-
740343
Gibson
A structural phylogeny for und ...
Desulfovibrio africanus, Saccharolobus solfataricus
Curr. Opin. Struct. Biol.
41
54-61
2016
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
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2
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2
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2
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2
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740367
Li
-
The catalytic bias of 2-oxoaci ...
Desulfovibrio africanus, Hydrogenobacter thermophilus
Electrochim. Acta
199
349-356
2016
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724470
Yan
Four Cys residues in heterodim ...
Sulfurisphaera tokodaii
Biochim. Biophys. Acta
1844
736-743
2014
-
-
-
-
5
-
-
-
-
1
-
1
-
3
-
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
740620
Yokooji
Genetic examination of initial ...
Thermococcus kodakarensis
J. Bacteriol.
195
1940-1948
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718962
Luo
Identification of the lysine r ...
Sulfurisphaera tokodaii
Biochim. Biophys. Acta
1794
335-340
2009
-
-
1
-
2
-
1
3
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
674385
Park
Purifications and characteriza ...
Saccharolobus solfataricus, Saccharolobus solfataricus DSM 1616, Saccharolobus solfataricus P1
J. Biochem. Mol. Biol.
39
46-54
2006
-
-
-
-
-
-
-
4
-
-
3
-
-
16
-
-
1
-
-
-
1
-
18
2
2
-
-
5
2
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
3
-
-
-
-
1
-
-
1
-
18
2
2
-
-
5
2
1
-
-
-
-
-
-
6
6
673610
Nishizawa
Gene expression and characteri ...
Aeropyrum pernix
FEBS Lett.
579
2319-2322
2005
-
-
1
-
-
-
1
2
-
-
5
1
-
2
-
-
1
-
-
-
-
-
12
1
2
1
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
-
-
5
1
-
-
-
1
-
-
-
-
12
1
2
1
1
-
1
1
-
-
-
-
-
-
-
-
654838
Fukuda
Substrate recognition by 2-oxo ...
Sulfolobus sp., Sulfolobus sp. 7
Biochim. Biophys. Acta
1597
74-80
2002
-
-
-
-
-
-
-
6
-
-
-
-
-
16
-
-
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
719399
Fukuda
Role of a highly conserved YPI ...
Sulfolobus sp., Sulfolobus sp. 7
Eur. J. Biochem.
268
5639-5646
2001
-
-
1
-
14
-
-
20
-
1
3
-
-
17
-
-
1
-
-
-
-
-
6
1
2
-
2
21
2
-
-
1
-
-
-
-
-
1
1
-
14
-
-
-
-
20
-
1
3
-
-
-
-
1
-
-
-
-
6
1
2
-
2
21
2
-
-
-
-
-
-
-
22
22
725876
Iwasaki
Ferredoxin and related enzymes ...
Sulfolobus sp., Sulfolobus sp. 7
Methods Enzymol.
334
3-22
2001
-
-
-
-
-
-
-
2
-
2
2
-
-
3
-
-
1
-
-
-
-
-
6
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
2
-
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288435
Zhang
2-Oxoacid:ferredoxin oxidoredu ...
Sulfolobus sp. 7, Sulfolobus sp.
J. Biochem.
120
587-599
1996
-
-
1
-
-
-
-
2
-
2
-
-
-
9
1
-
1
-
-
-
-
2
6
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
2
-
-
-
1
-
1
-
-
-
2
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
725332
Iwasaki
Ferredoxin-dependent redox sys ...
Sulfolobus sp., Sulfolobus sp. 7
J. Biol. Chem.
270
17878-17883
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
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-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
288417
Kerscher
Purification and properties of ...
Halobacterium salinarum
Eur. J. Biochem.
116
587-594
1981
-
-
-
-
-
1
1
4
-
1
3
-
-
2
-
-
1
-
-
-
-
-
3
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
4
-
1
3
-
-
-
-
1
-
-
-
-
3
1
1
1
-
-
1
1
-
-
-
-
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-
-
-