Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | stimulatory | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: carbon monoxide, sodium cyanide, sodium fluoride, sodium azide | Thermotoga maritima | |
Sodium nitrite | - |
Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
CoA | pH 8.2, 80°C | Thermotoga maritima | |
14.5 | - |
pyruvate | pH 8.2, 80°C | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE | Thermotoga maritima |
23000 | - |
x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE | Thermotoga maritima |
34000 | - |
x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE | Thermotoga maritima |
43000 | - |
x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE | Thermotoga maritima |
155000 | - |
gel fitlration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Oxidation Stability | Organism |
---|---|
25°C, half-life in presence of air 70 min | Thermotoga maritima |
Purification (Comment) | Organism |
---|---|
purification under strictly unaerobic conditions | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CoA is absolutely required. No substrates: 2-oxoglutarate, phenyl pyruvate, or indolyl pyruvate | Thermotoga maritima | ? | - |
? | |
pyruvate + CoA + oxidized methyl viologen | - |
Thermotoga maritima | acetyl-CoA + CO2 + reduced methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life 15 h | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | proteins contains at least two ferredoxin-type [4Fe-4S] clusters per molecule. Km value for ferredoxin is 0.026 microM | Thermotoga maritima |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
54 | - |
Sodium nitrite | pH 8.2, 80°C | Thermotoga maritima |