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Literature summary for 1.2.7.1 extracted from
- The bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase from Thermococcus guaymasensis (2014), Archaea, 2014, 349379.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
CoA | pH 8.4, 80°C | Thermococcus guaymasensis |  |
| 0.25 | - |
pyruvate | pH 8.4, 80°C | Thermococcus guaymasensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
| 26000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
| 35000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
| 46000 | - |
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
| 258000 | - |
gel filtration | Thermococcus guaymasensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA + 2 oxidized ferredoxin | Thermococcus guaymasensis | - |
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
| pyruvate + CoA + 2 oxidized ferredoxin | Thermococcus guaymasensis DSM 11113 | - |
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus guaymasensis | W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 | W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta | - |
| Thermococcus guaymasensis DSM 11113 | W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 | W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| oxygen-sensitive enzyme, the t1/2 for the enzyme activity in 50 mM Tris-HCl, pH 7.8 containing 2 mM sodium dithionite and 2 mM DTT is approximately 30 min | Thermococcus guaymasensis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus guaymasensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.2 | - |
purified enzyme, pH 8.4, 80°C | Thermococcus guaymasensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA + 2 oxidized ferredoxin | - |
Thermococcus guaymasensis | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
| pyruvate + CoA + 2 oxidized ferredoxin | - |
Thermococcus guaymasensis DSM 11113 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE | Thermococcus guaymasensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase | - |
Thermococcus guaymasensis |
| POR | - |
Thermococcus guaymasensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
above | Thermococcus guaymasensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Thermococcus guaymasensis |
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Release 2023.1 (January 2023)
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