Application | Comment | Organism |
---|---|---|
synthesis | establishment of a functional heterologous expression system of Moco-free apo-CODH in Escherichia coli. The expression of the CoxMSL genes alone results in a colorless and inactive protein that lacks the cofactors. Expression of an active protein requires coexpression of CoxI | Afipia carboxidovorans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Afipia carboxidovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00052 | - |
CO | pH 7.2, 30°C | Afipia carboxidovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | - |
Afipia carboxidovorans | |
Molybdenum | - |
Afipia carboxidovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
285000 | - |
gel filtration | Afipia carboxidovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Afipia carboxidovorans | P19921 and P19920 and P19919 | P19921 i.e. small subunit CoxS, P19920 i.e. medium subunit CoxM, P19919 i.e. large subunit CoxL | - |
Afipia carboxidovorans DSM 1227 | P19921 and P19920 and P19919 | P19921 i.e. small subunit CoxS, P19920 i.e. medium subunit CoxM, P19919 i.e. large subunit CoxL | - |
Purification (Comment) | Organism |
---|---|
recombinant protein, purification by Ni-NTA and size exclusion chromatography | Afipia carboxidovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
recombinant purified enzyme, pH 7.2, 30°C | Afipia carboxidovorans |
2.6 | - |
recombinant purified enzyme, reconstitution of the active site with sulfur and copper, pH 7.2, 30°C | Afipia carboxidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride + H2O | - |
Afipia carboxidovorans | CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride | - |
? | |
CO + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride + H2O | - |
Afipia carboxidovorans DSM 1227 | CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride | - |
? | |
additional information | is able to catalyze both the oxidation of CO to CO2 and the oxidation of H2 to protons and electrons | Afipia carboxidovorans | ? | - |
- |
|
additional information | is able to catalyze both the oxidation of CO to CO2 and the oxidation of H2 to protons and electrons | Afipia carboxidovorans DSM 1227 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
CoxS | - |
Afipia carboxidovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.6 | - |
CO | pH 7.2, 30°C | Afipia carboxidovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[2Fe-2S]-center | presence of 2 [Fe2S2] clusters, UV-vis spectrum shows a shoulder at 550 nm | Afipia carboxidovorans |