BRENDA - Enzyme Database
show all sequences of 1.2.5.3

Orbital contributions to CO oxidation in Mo-Cu carbon monoxide dehydrogenase

Stein, B.; Kirk, M.; Chem. Commun. (Camb.) 50, 1104-1106 (2014)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
n-butylisocyanide
-
Hydrogenophaga pseudoflava
n-butylisocyanide
-
Oligotropha carboxidovorans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu
-
Hydrogenophaga pseudoflava
Cu
-
Oligotropha carboxidovorans
Mo
-
Hydrogenophaga pseudoflava
Mo
-
Oligotropha carboxidovorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CO + a quinone + H2O
Hydrogenophaga pseudoflava
-
CO2 + a quinol
-
-
?
CO + a quinone + H2O
Oligotropha carboxidovorans
-
CO2 + a quinol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hydrogenophaga pseudoflava
P19913 and P19914 and P19915
large, medium, and small subunit
-
Oligotropha carboxidovorans
P19920
-
-
Reaction
Reaction
Commentary
Organism
CO + a quinone + H2O = CO2 + a quinol
reaction mechanism that initially involves nucleophilic attack of a Mo=O oxo on the carbon center of Cu(I)-CO, resulting in a 5-membered cyclic m2-e(2) CO2-bridged Mo(VI)-Cu(I)-Cys complex I that can bind HO-/H2O to yield 1-OH. This is followed by a second nucleophilic attack on the activated mu2-nu2 CO2 carbon centre of 1-OH to yield a Mo(IV)-bicarbonate product complex, 1-P. This second nucleophilic attack is suggested based on electronic structure description of cyclic m2-e(2) CO2-bridged Mo(VI)-Cu(I)-Cys complex I, which possesses a bent and activated CO2 bound to the Mo and Cu ions. Proposed catalytic cycle for CODH that avoids formation of a stable C-S bonded cyclic m2-e(2) CO2-bridged Mo(VI)-Cu(I)-Cys complex II
Hydrogenophaga pseudoflava
CO + a quinone + H2O = CO2 + a quinol
reaction mechanism that initially involves nucleophilic attack of a Mo=O oxo on the carbon center of Cu(I)-CO, resulting in a 5-membered cyclic m2-e(2) CO2-bridged Mo(VI)-Cu(I)-Cys complex I that can bind HO-/H2O to yield 1-OH. This is followed by a second nucleophilic attack on the activated mu2-nu2 CO2 carbon centre of 1-OH to yield a Mo(IV)-bicarbonate product complex, 1-P. This second nucleophilic attack is suggested based on our electronic structure description of cyclic m2-e(2) CO2-bridged Mo(VI)-Cu(I)-Cys complex I, which possesses a bent and activated CO2 bound to the Mo and Cu ions. Proposed catalytic cycle for CODH that avoids formation of a stable C-S bonded cyclic m2-e(2) CO2-bridged Mo(VI)-Cu(I)-Cys complex II
Oligotropha carboxidovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CO + a quinone + H2O
-
735935
Hydrogenophaga pseudoflava
CO2 + a quinol
-
-
-
?
CO + a quinone + H2O
-
735935
Oligotropha carboxidovorans
CO2 + a quinol
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
quinone
-
Hydrogenophaga pseudoflava
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
quinone
-
Hydrogenophaga pseudoflava
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
n-butylisocyanide
-
Hydrogenophaga pseudoflava
n-butylisocyanide
-
Oligotropha carboxidovorans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu
-
Hydrogenophaga pseudoflava
Cu
-
Oligotropha carboxidovorans
Mo
-
Hydrogenophaga pseudoflava
Mo
-
Oligotropha carboxidovorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CO + a quinone + H2O
Hydrogenophaga pseudoflava
-
CO2 + a quinol
-
-
?
CO + a quinone + H2O
Oligotropha carboxidovorans
-
CO2 + a quinol
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CO + a quinone + H2O
-
735935
Hydrogenophaga pseudoflava
CO2 + a quinol
-
-
-
?
CO + a quinone + H2O
-
735935
Oligotropha carboxidovorans
CO2 + a quinol
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
the enzyme is a member of the xanthine oxidase (XO) family of pyranopterin molybdenum enzymes that typically catalyse the oxidative hydroxylation of N-heterocyle and aldehyde
Hydrogenophaga pseudoflava
evolution
the enzyme is a member of the xanthine oxidase (XO) family of pyranopterin molybdenum enzymes that typically catalyse the oxidative hydroxylation of N-heterocyle and aldehyde substrates
Oligotropha carboxidovorans
additional information
mechanism of Mo/Cu carbon monoxide dehydrogenase, electronic structure contributions to reactivity, overview
Hydrogenophaga pseudoflava
additional information
mechanism of Mo/Cu carbon monoxide dehydrogenase, electronic structure contributions to reactivity, overview
Oligotropha carboxidovorans
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is a member of the xanthine oxidase (XO) family of pyranopterin molybdenum enzymes that typically catalyse the oxidative hydroxylation of N-heterocyle and aldehyde
Hydrogenophaga pseudoflava
evolution
the enzyme is a member of the xanthine oxidase (XO) family of pyranopterin molybdenum enzymes that typically catalyse the oxidative hydroxylation of N-heterocyle and aldehyde substrates
Oligotropha carboxidovorans
additional information
mechanism of Mo/Cu carbon monoxide dehydrogenase, electronic structure contributions to reactivity, overview
Hydrogenophaga pseudoflava
additional information
mechanism of Mo/Cu carbon monoxide dehydrogenase, electronic structure contributions to reactivity, overview
Oligotropha carboxidovorans
Other publictions for EC 1.2.5.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736514
Hille
The aerobic CO dehydrogenase f ...
Oligotropha carboxidovorans
J. Biol. Inorg. Chem.
20
243-251
2015
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1
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1
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4
4
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735935
Stein
Orbital contributions to CO ox ...
Hydrogenophaga pseudoflava, Oligotropha carboxidovorans
Chem. Commun. (Camb.)
50
1104-1106
2014
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4
4
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736513
Pelzmann
Insights into the posttranslat ...
Oligotropha carboxidovorans
J. Biol. Inorg. Chem.
19
1399-1414
2014
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1
1
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4
4
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736437
Wilcoxen
The hydrogenase activity of th ...
Oligotropha carboxidovorans
J. Biol. Chem.
288
36052-36060
2013
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1
1
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1
1
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725197
Wilcoxen
Substitution of silver for cop ...
Oligotropha carboxidovorans
J. Am. Chem. Soc.
133
12934-12936
2011
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1
1
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1
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3
3
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735667
Wilcoxen
Reaction of the molybdenum- an ...
, Oligotropha carboxidovorans ATCC 49405
Biochemistry
50
1910-1916
2011
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2
2
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725052
Nishimura
-
Purification and characterizat ...
Aeropyrum pernix, Aeropyrum pernix TB5
Fish. Sci.
76
999-1006
2010
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2
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725392
Zhang
Kinetic and spectroscopic stud ...
Oligotropha carboxidovorans
J. Biol. Chem.
285
12571-12578
2010
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736301
Gourlay
Paramagnetic active site model ...
Oligotropha carboxidovorans
J. Am. Chem. Soc.
128
2164-2165
2006
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2
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736512
Resch
Structural and functional reco ...
Oligotropha carboxidovorans, Oligotropha carboxidovorans DSM 1227
J. Biol. Inorg. Chem.
10
518-528
2005
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390484
Lorite
Carbon monoxide dehydrogenase ...
Bradyrhizobium japonicum, Bradyrhizobium japonicum 110spc4
Appl. Environ. Microbiol.
66
1871-1876
2000
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656481
Hanzelmann
The effect of intracellular mo ...
Hydrogenophaga pseudoflava, Hydrogenophaga pseudoflava DSM 1084
J. Mol. Biol.
301
1221-1235
2000
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1
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736397
Gremer
Binding of flavin adenine dinu ...
Oligotropha carboxidovorans, Oligotropha carboxidovorans DSM 1227
J. Biol. Chem.
275
1864-1872
2000
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4
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2
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390482
Dobbek
Crystal structure and mechanis ...
Oligotropha carboxidovorans
Proc. Natl. Acad. Sci. USA
96
8884-8889
1999
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1
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390483
Kang
Cloning and molecular characte ...
Hydrogenophaga pseudoflava, Hydrogenophaga pseudoflava DSM 1084
J. Bacteriol.
181
5581-5590
1999
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390479
Schubel
Molecular characterization of ...
Oligotropha carboxidovorans, Oligotropha carboxidovorans OM5
J. Bacteriol.
177
2197-2203
1995
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