Cloned (Comment) | Organism |
---|---|
genes cutM, cutS, and cutL, gene cluster cutMSL, DNA and amino acid sequence determination and anakysis, sequence comparisons, genetic organization | Hydrogenophaga pseudoflava |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Hydrogenophaga pseudoflava | |
iron-sulfur center | two [2Fe-2S] clusters in the small subunit | Hydrogenophaga pseudoflava | |
Molybdenum | - |
Hydrogenophaga pseudoflava |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17752 | - |
x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation | Hydrogenophaga pseudoflava |
30694 | - |
x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation | Hydrogenophaga pseudoflava |
87224 | - |
x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation | Hydrogenophaga pseudoflava |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenophaga pseudoflava | P19913 and P19914 and P19915 | large, medium, and small subunits; genes cutM, cutS, and cutL | - |
Hydrogenophaga pseudoflava DSM 1084 | P19913 and P19914 and P19915 | large, medium, and small subunits; genes cutM, cutS, and cutL | - |
Purification (Comment) | Organism |
---|---|
native enzyme by ammonium sulfate fractionation, dialysis, and anion exchange chromatography, followed by ultrafiltration, and gel filtration | Hydrogenophaga pseudoflava |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride + H2O | - |
Hydrogenophaga pseudoflava | CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride | - |
? | |
CO + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride + H2O | - |
Hydrogenophaga pseudoflava DSM 1084 | CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation | Hydrogenophaga pseudoflava |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hydrogenophaga pseudoflava |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Hydrogenophaga pseudoflava |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | in the medium subunit | Hydrogenophaga pseudoflava | |
molybdenum cofactor | in the large subunit | Hydrogenophaga pseudoflava |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hydrogenophaga pseudoflava | large subunit, sequence calculation | - |
5.88 |
Hydrogenophaga pseudoflava | small subunit, sequence calculation | - |
7.77 |
Hydrogenophaga pseudoflava | medium subunit, sequence calculation | - |
8.43 |
General Information | Comment | Organism |
---|---|---|
evolution | the sequences of CutMSL are highly conserved in CO-DHs and other molybdenum-containing hydroxylases | Hydrogenophaga pseudoflava |