BRENDA - Enzyme Database
show all sequences of 1.2.5.2

Molecular and catalytic properties of the aldehyde dehydrogenase of Gluconacetobacter diazotrophicus, a quinoheme protein containing pyrroloquinoline quinone, cytochrome b, and cytochrome c

Gomez-Manzo, S.; Chavez-Pacheco, J.L.; Contreras-Zentella, M.; Sosa-Torres, M.E.; Arreguin-Espinosa, R.; Perez de la Mora, M.; Membrillo-Hernandez, J.; Escamilla, J.E.; J. Bacteriol. 192, 5718-5724 (2010) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CuSO4
1 mM, cosubstate ferricyanide, strong inhibition; 1 mM, strong inhibition
Gluconacetobacter diazotrophicus
HgCl2
1 mM, cosubstate ferricyanide, strong inhibition; 1 mM, strong inhibition
Gluconacetobacter diazotrophicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.3
-
acetic acid
cosubstrate ferricyandie, pH 3.5, 30°C
Gluconacetobacter diazotrophicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-bound complex
Gluconacetobacter diazotrophicus
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions
Gluconacetobacter diazotrophicus
79700
-
1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions
Gluconacetobacter diazotrophicus
79700
-
1 * 79700 and 1 * 50000,SDS-PAGE
Gluconacetobacter diazotrophicus
129000
-
PAGE
Gluconacetobacter diazotrophicus
129000
-
nondenaturing PAGE
Gluconacetobacter diazotrophicus
Organism
Organism
UniProt
Commentary
Textmining
Gluconacetobacter diazotrophicus
-
-
-
Gluconacetobacter diazotrophicus ATCC 49037
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Gluconacetobacter diazotrophicus
Storage Stability
Storage Stability
Organism
-70°C, stable for several months
Gluconacetobacter diazotrophicus
-70°C, stable for weeks without appreciable loss of activity
Gluconacetobacter diazotrophicus
4°C, stable for several weeks
Gluconacetobacter diazotrophicus
4°C, stable for several weeks without appreciable loss of activity
Gluconacetobacter diazotrophicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetaldehyde + 2 ferricyanide + H2O
-
731999
Gluconacetobacter diazotrophicus
acetate + 2 ferrocyanide + 2 H+
-
-
-
?
acetaldehyde + 2 ferricyanide + H2O
-
731999
Gluconacetobacter diazotrophicus ATCC 49037
acetate + 2 ferrocyanide + 2 H+
-
-
-
?
acetaldehyde + ferricyanide
-
731999
Gluconacetobacter diazotrophicus
acetic acid + ferrocyanide + H+
-
-
-
?
acetaldehyde + ferricyanide
-
731999
Gluconacetobacter diazotrophicus ATCC 49037
acetic acid + ferrocyanide + H+
-
-
-
?
acetaldehyde + oxidized phenazine methosulfate
-
731999
Gluconacetobacter diazotrophicus
acetic acid + reduced phenazine methosulfate
-
-
-
?
acetaldehyde + oxidized phenazine methosulfate
-
731999
Gluconacetobacter diazotrophicus ATCC 49037
acetic acid + reduced phenazine methosulfate
-
-
-
?
glutaraldehyde + 2 ferricyanide + H2O
26.4% compared to the activity with acetaldehyde
731999
Gluconacetobacter diazotrophicus
glutarate + 2 ferrocyanide + 2 H+
-
-
-
?
glutaraldehyde + oxidized phenazine methosulfate
26.5% of the activity with acetaldehyde
731999
Gluconacetobacter diazotrophicus
glutaric acid + reduced phenazine methosulfate
-
-
-
?
additional information
formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme
731999
Gluconacetobacter diazotrophicus
?
-
-
-
?
additional information
no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes
731999
Gluconacetobacter diazotrophicus
?
-
-
-
?
additional information
formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme
731999
Gluconacetobacter diazotrophicus ATCC 49037
?
-
-
-
?
additional information
no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes
731999
Gluconacetobacter diazotrophicus ATCC 49037
?
-
-
-
?
propanal + oxidized phenazine methosulfate
72.7% of the activity with acetaldehyde
731999
Gluconacetobacter diazotrophicus
propanoic acid + reduced phenazine methosulfate
-
-
-
?
propionaldehyde + 2 ferricyanide + H2O
72.7% compared to the activity with acetaldehyde
731999
Gluconacetobacter diazotrophicus
propionate + 2 ferrocyanide + 2 H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterodimer
1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions
Gluconacetobacter diazotrophicus
heterodimer
1 * 79700 and 1 * 50000,SDS-PAGE
Gluconacetobacter diazotrophicus
Synonyms
Synonyms
Commentary
Organism
aldehyde dehydrogenase (acceptor)
-
Gluconacetobacter diazotrophicus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
22000
-
acetic acid
cosubstrate ferricyandie, pH 3.5, 30°C
Gluconacetobacter diazotrophicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5
-
electron accceptor ferricyanide
Gluconacetobacter diazotrophicus
3.5
-
using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors
Gluconacetobacter diazotrophicus
7
-
electron acceptor phenazine methosulfate plus 2,6-dichlorophenolindophenol
Gluconacetobacter diazotrophicus
7
-
using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors
Gluconacetobacter diazotrophicus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b
-
Gluconacetobacter diazotrophicus
cytochrome b
the enzyme complex contains one cytochrome b
Gluconacetobacter diazotrophicus
cytochrome c
the smaller subunit bears three cytochromes c
Gluconacetobacter diazotrophicus
pyrroloquinoline quinone
-
Gluconacetobacter diazotrophicus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Gluconacetobacter diazotrophicus
isoelectric focusing
-
5.2
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b
-
Gluconacetobacter diazotrophicus
cytochrome b
the enzyme complex contains one cytochrome b
Gluconacetobacter diazotrophicus
cytochrome c
the smaller subunit bears three cytochromes c
Gluconacetobacter diazotrophicus
pyrroloquinoline quinone
-
Gluconacetobacter diazotrophicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuSO4
1 mM, strong inhibition
Gluconacetobacter diazotrophicus
CuSO4
1 mM, cosubstate ferricyanide, strong inhibition
Gluconacetobacter diazotrophicus
HgCl2
1 mM, strong inhibition
Gluconacetobacter diazotrophicus
HgCl2
1 mM, cosubstate ferricyanide, strong inhibition
Gluconacetobacter diazotrophicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.3
-
acetic acid
cosubstrate ferricyandie, pH 3.5, 30°C
Gluconacetobacter diazotrophicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-bound complex
Gluconacetobacter diazotrophicus
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions
Gluconacetobacter diazotrophicus
79700
-
1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions
Gluconacetobacter diazotrophicus
79700
-
1 * 79700 and 1 * 50000,SDS-PAGE
Gluconacetobacter diazotrophicus
129000
-
PAGE
Gluconacetobacter diazotrophicus
129000
-
nondenaturing PAGE
Gluconacetobacter diazotrophicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gluconacetobacter diazotrophicus
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, stable for several months
Gluconacetobacter diazotrophicus
-70°C, stable for weeks without appreciable loss of activity
Gluconacetobacter diazotrophicus
4°C, stable for several weeks
Gluconacetobacter diazotrophicus
4°C, stable for several weeks without appreciable loss of activity
Gluconacetobacter diazotrophicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetaldehyde + 2 ferricyanide + H2O
-
731999
Gluconacetobacter diazotrophicus
acetate + 2 ferrocyanide + 2 H+
-
-
-
?
acetaldehyde + 2 ferricyanide + H2O
-
731999
Gluconacetobacter diazotrophicus ATCC 49037
acetate + 2 ferrocyanide + 2 H+
-
-
-
?
acetaldehyde + ferricyanide
-
731999
Gluconacetobacter diazotrophicus
acetic acid + ferrocyanide + H+
-
-
-
?
acetaldehyde + ferricyanide
-
731999
Gluconacetobacter diazotrophicus ATCC 49037
acetic acid + ferrocyanide + H+
-
-
-
?
acetaldehyde + oxidized phenazine methosulfate
-
731999
Gluconacetobacter diazotrophicus
acetic acid + reduced phenazine methosulfate
-
-
-
?
acetaldehyde + oxidized phenazine methosulfate
-
731999
Gluconacetobacter diazotrophicus ATCC 49037
acetic acid + reduced phenazine methosulfate
-
-
-
?
glutaraldehyde + 2 ferricyanide + H2O
26.4% compared to the activity with acetaldehyde
731999
Gluconacetobacter diazotrophicus
glutarate + 2 ferrocyanide + 2 H+
-
-
-
?
glutaraldehyde + oxidized phenazine methosulfate
26.5% of the activity with acetaldehyde
731999
Gluconacetobacter diazotrophicus
glutaric acid + reduced phenazine methosulfate
-
-
-
?
additional information
formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme
731999
Gluconacetobacter diazotrophicus
?
-
-
-
?
additional information
no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes
731999
Gluconacetobacter diazotrophicus
?
-
-
-
?
additional information
formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme
731999
Gluconacetobacter diazotrophicus ATCC 49037
?
-
-
-
?
additional information
no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes
731999
Gluconacetobacter diazotrophicus ATCC 49037
?
-
-
-
?
propanal + oxidized phenazine methosulfate
72.7% of the activity with acetaldehyde
731999
Gluconacetobacter diazotrophicus
propanoic acid + reduced phenazine methosulfate
-
-
-
?
propionaldehyde + 2 ferricyanide + H2O
72.7% compared to the activity with acetaldehyde
731999
Gluconacetobacter diazotrophicus
propionate + 2 ferrocyanide + 2 H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions
Gluconacetobacter diazotrophicus
heterodimer
1 * 79700 and 1 * 50000,SDS-PAGE
Gluconacetobacter diazotrophicus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
22000
-
acetic acid
cosubstrate ferricyandie, pH 3.5, 30°C
Gluconacetobacter diazotrophicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5
-
electron accceptor ferricyanide
Gluconacetobacter diazotrophicus
3.5
-
using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors
Gluconacetobacter diazotrophicus
7
-
electron acceptor phenazine methosulfate plus 2,6-dichlorophenolindophenol
Gluconacetobacter diazotrophicus
7
-
using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors
Gluconacetobacter diazotrophicus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Gluconacetobacter diazotrophicus
isoelectric focusing
-
5.2
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6720
-
acetic acid
cosubstrate ferricyandie, pH 3.5, 30°C
Gluconacetobacter diazotrophicus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6720
-
acetic acid
cosubstrate ferricyandie, pH 3.5, 30°C
Gluconacetobacter diazotrophicus
Other publictions for EC 1.2.5.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731507
Aquino Neto
High current density PQQ-depen ...
Gluconobacter oxydans, Gluconobacter oxydans DSM 3504
Biosens. Bioelectron.
72
247-254
2015
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731204
Zeiser
Characterization of a periplas ...
Sphingomonas wittichii, Sphingomonas wittichii DSM 6014
Appl. Microbiol. Biotechnol.
98
2067-2079
2014
-
-
1
-
-
-
4
1
1
-
8
-
-
9
-
-
-
-
-
-
9
-
15
2
2
1
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
4
-
1
1
-
8
-
-
-
-
-
-
-
9
-
15
2
1
-
-
-
1
-
1
-
-
1
1
-
1
1
724837
Aquino Neto
-
Direct electron transfer-based ...
Gluconobacter sp., Gluconobacter sp. 33 (DSM Z 3504)
Electrochim. Acta
87
323-329
2013
-
-
-
-
-
-
-
1
-
-
3
-
-
2
-
-
1
-
-
1
-
2
6
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
1
-
2
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731035
Xu
-
Investigating the impact of mu ...
Gluconobacter oxydans, Gluconobacter oxydans DSM 3504
ACS Catal.
3
1756-1763
2013
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731999
Gomez-Manzo
Molecular and catalytic proper ...
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus ATCC 49037
J. Bacteriol.
192
5718-5724
2010
-
-
-
-
-
-
2
1
1
-
5
-
-
2
-
-
1
-
-
-
-
4
14
2
1
-
-
-
1
4
-
-
4
-
1
-
-
-
-
4
-
-
-
-
4
-
1
1
-
5
-
-
-
-
1
-
-
-
4
14
2
-
-
-
1
4
-
-
1
-
-
-
-
1
1
687166
Gomez-Manzo
The PQQ-alcohol dehydrogenase ...
Gluconacetobacter diazotrophicus
Int. J. Food Microbiol.
125
71-78
2008
-
-
-
-
-
-
-
-
1
-
3
1
-
1
-
-
1
-
-
-
1
-
5
1
-
-
-
1
-
1
1
-
2
-
1
-
-
-
-
2
-
-
-
-
-
-
-
1
-
3
1
-
-
-
1
-
-
1
-
5
1
-
-
1
-
1
1
-
1
-
-
-
-
-
-
685650
Shinagawa
A novel type of formaldehyde-o ...
Acetobacter sp., Acetobacter sp. SKU 14
Biosci. Biotechnol. Biochem.
70
850-857
2006
-
-
-
-
-
-
-
1
3
-
6
-
-
7
-
-
2
-
-
1
1
-
15
2
2
2
1
3
-
2
1
8
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
3
-
6
-
-
-
-
2
-
1
1
-
15
2
2
1
3
-
2
1
8
-
-
-
-
-
-
-
687366
Vangnai
Two distinct alcohol dehydroge ...
Thauera butanivorans
J. Bacteriol.
184
1916-1924
2002
1
-
1
-
1
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
3
1
2
-
-
-
-
-
-
-
2
-
-
-
1
-
1
2
-
1
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389961
Zarnt
Catalytic and molecular proper ...
Cupriavidus necator, Cupriavidus necator Bo
J. Bacteriol.
183
1954-1960
2001
-
-
1
-
-
-
3
5
-
-
1
-
-
7
-
-
-
-
-
-
-
-
10
1
1
-
-
-
-
-
-
-
1
3
-
-
-
-
1
1
-
-
-
-
3
3
5
-
-
1
-
-
-
-
-
-
-
-
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688853
Vangnai
An inducible 1-butanol dehydro ...
Thauera butanivorans
Microbiology
147
745-756
2001
2
-
-
-
-
-
-
1
1
1
2
2
-
1
-
-
1
-
1
4
4
1
10
1
-
1
-
2
-
1
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
1
1
1
2
2
-
-
-
1
1
4
4
1
10
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
684539
Zarnt
Degradation of tetrahydrofurfu ...
Cupriavidus necator
Appl. Environ. Microbiol.
63
4891-4898
1997
1
-
-
-
-
-
1
-
-
1
2
-
-
1
-
-
1
-
-
1
1
1
6
1
-
-
-
-
-
-
-
-
2
-
1
-
1
-
-
2
-
-
-
-
1
-
-
-
1
2
-
-
-
-
1
-
1
1
1
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
687362
Toyama
Three distinct quinoprotein al ...
Pseudomonas putida, Pseudomonas putida HK5
J. Bacteriol.
177
2442-2450
1995
3
-
-
-
-
-
1
-
3
-
6
6
-
9
-
-
3
-
-
3
4
-
28
3
4
-
-
-
-
3
-
-
6
-
3
-
3
-
-
6
-
-
-
-
1
-
-
3
-
6
6
-
-
-
3
-
3
4
-
28
3
-
-
-
-
3
-
-
3
-
-
-
-
-
-
389935
Görisch
Quinoprotein ethanol dehydroge ...
Pseudomonas aeruginosa, Pseudomonas putida
Antonie van Leeuwenhoek
56
35-45
1989
-
-
-
2
-
-
-
-
-
-
2
-
-
2
-
-
2
-
-
-
-
-
7
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288470
Adachi
-
Preparation of cells and cytop ...
Acetobacter aceti
Agric. Biol. Chem.
52
2083-2084
1988
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
661296
Rupp
Purification, crystallisation ...
Pseudomonas aeruginosa
Biol. Chem. Hoppe-Seyler
369
431-439
1988
-
-
-
1
-
-
-
1
-
-
3
-
-
1
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
389953
Groen
Quinohaemoprotein alcohol dehy ...
Comamonas testosteroni
Biochem. J.
234
611-615
1986
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
1
5
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288472
Hommel
-
A pyridine nucleotide-independ ...
Acetobacter pasteurianus
FEMS Microbiol. Lett.
22
139-142
1984
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
5
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
5
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389951
Groen
Quinoprotein alcohol dehydroge ...
Pseudomonas aeruginosa
Biochem. J.
223
921-924
1984
-
-
-
-
-
-
-
3
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288471
Ameyama
-
Aldehyde dehydrogenase from ac ...
Acetobacter aceti, Gluconobacter oxydans
Methods Enzymol.
89
491-497
1982
-
-
-
-
-
-
-
2
-
-
2
2
-
2
-
-
2
-
-
-
2
2
8
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
2
-
-
-
2
-
-
2
2
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
288474
Ameyama
-
Purification and characterizat ...
Acetobacter aceti
Agric. Biol. Chem.
45
1889-1890
1981
-
-
-
-
-
1
-
1
1
-
3
-
-
1
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
1
-
3
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-