Reference to 1.2.5.2; Id = 731999 - BRENDA Enzyme Database

CuSO4

1 mM, cosubstate ferricyanide, strong inhibition; 1 mM, strong inhibition

Gluconacetobacter diazotrophicus

HgCl2

1 mM, cosubstate ferricyanide, strong inhibition; 1 mM, strong inhibition

Gluconacetobacter diazotrophicus

3.3

-

acetic acid

cosubstrate ferricyandie, pH 3.5, 30Â°C

Gluconacetobacter diazotrophicus

membrane

membrane-bound complex

Gluconacetobacter diazotrophicus

16020

-

50000

-

1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions

Gluconacetobacter diazotrophicus

79700

-

1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions

Gluconacetobacter diazotrophicus

79700

-

1 * 79700 and 1 * 50000,SDS-PAGE

Gluconacetobacter diazotrophicus

129000

-

PAGE

Gluconacetobacter diazotrophicus

129000

-

nondenaturing PAGE

Gluconacetobacter diazotrophicus

-

Gluconacetobacter diazotrophicus ATCC 49037

-

Gluconacetobacter diazotrophicus

-70Â°C, stable for several months

Gluconacetobacter diazotrophicus

-70Â°C, stable for weeks without appreciable loss of activity

Gluconacetobacter diazotrophicus

4Â°C, stable for several weeks

Gluconacetobacter diazotrophicus

4Â°C, stable for several weeks without appreciable loss of activity

Gluconacetobacter diazotrophicus

acetaldehyde + 2 ferricyanide + H2O

-

731999

Gluconacetobacter diazotrophicus

acetate + 2 ferrocyanide + 2 H+

-

?

acetaldehyde + 2 ferricyanide + H2O

-

731999

Gluconacetobacter diazotrophicus ATCC 49037

acetate + 2 ferrocyanide + 2 H+

-

?

acetaldehyde + ferricyanide

-

731999

Gluconacetobacter diazotrophicus

acetic acid + ferrocyanide + H+

-

?

acetaldehyde + ferricyanide

-

731999

Gluconacetobacter diazotrophicus ATCC 49037

acetic acid + ferrocyanide + H+

-

?

acetaldehyde + oxidized phenazine methosulfate

-

731999

Gluconacetobacter diazotrophicus

acetic acid + reduced phenazine methosulfate

-

?

acetaldehyde + oxidized phenazine methosulfate

-

731999

Gluconacetobacter diazotrophicus ATCC 49037

acetic acid + reduced phenazine methosulfate

-

?

glutaraldehyde + 2 ferricyanide + H2O

26.4% compared to the activity with acetaldehyde

731999

Gluconacetobacter diazotrophicus

glutarate + 2 ferrocyanide + 2 H+

-

?

glutaraldehyde + oxidized phenazine methosulfate

26.5% of the activity with acetaldehyde

731999

Gluconacetobacter diazotrophicus

glutaric acid + reduced phenazine methosulfate

-

?

additional information

formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme

731999

Gluconacetobacter diazotrophicus

?

-

?

additional information

no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes

731999

Gluconacetobacter diazotrophicus

?

-

?

additional information

formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme

731999

Gluconacetobacter diazotrophicus ATCC 49037

?

-

?

additional information

no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes

731999

Gluconacetobacter diazotrophicus ATCC 49037

?

-

?

propanal + oxidized phenazine methosulfate

72.7% of the activity with acetaldehyde

731999

Gluconacetobacter diazotrophicus

propanoic acid + reduced phenazine methosulfate

-

?

propionaldehyde + 2 ferricyanide + H2O

72.7% compared to the activity with acetaldehyde

731999

Gluconacetobacter diazotrophicus

propionate + 2 ferrocyanide + 2 H+

-

?

heterodimer

1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions

Gluconacetobacter diazotrophicus

heterodimer

1 * 79700 and 1 * 50000,SDS-PAGE

Gluconacetobacter diazotrophicus

aldehyde dehydrogenase (acceptor)

-

Gluconacetobacter diazotrophicus

22000

-

acetic acid

cosubstrate ferricyandie, pH 3.5, 30Â°C

Gluconacetobacter diazotrophicus

3.5

-

electron accceptor ferricyanide

Gluconacetobacter diazotrophicus

3.5

-

using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors

Gluconacetobacter diazotrophicus

7

-

electron acceptor phenazine methosulfate plus 2,6-dichlorophenolindophenol

Gluconacetobacter diazotrophicus

7

-

using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors

Gluconacetobacter diazotrophicus

cytochrome b

-

Gluconacetobacter diazotrophicus

cytochrome b

the enzyme complex contains one cytochrome b

Gluconacetobacter diazotrophicus

cytochrome c

the smaller subunit bears three cytochromes c

Gluconacetobacter diazotrophicus

pyrroloquinoline quinone

-

Gluconacetobacter diazotrophicus

isoelectric focusing

-

5.2

cytochrome b

-

Gluconacetobacter diazotrophicus

cytochrome b

the enzyme complex contains one cytochrome b

Gluconacetobacter diazotrophicus

cytochrome c

the smaller subunit bears three cytochromes c

Gluconacetobacter diazotrophicus

pyrroloquinoline quinone

-

Gluconacetobacter diazotrophicus

CuSO4

1 mM, strong inhibition

Gluconacetobacter diazotrophicus

CuSO4

1 mM, cosubstate ferricyanide, strong inhibition

Gluconacetobacter diazotrophicus

HgCl2

1 mM, strong inhibition

Gluconacetobacter diazotrophicus

HgCl2

1 mM, cosubstate ferricyanide, strong inhibition

Gluconacetobacter diazotrophicus

3.3

-

acetic acid

cosubstrate ferricyandie, pH 3.5, 30Â°C

Gluconacetobacter diazotrophicus

membrane

membrane-bound complex

Gluconacetobacter diazotrophicus

16020

-

50000

-

1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions

Gluconacetobacter diazotrophicus

79700

-

1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions

Gluconacetobacter diazotrophicus

79700

-

1 * 79700 and 1 * 50000,SDS-PAGE

Gluconacetobacter diazotrophicus

129000

-

PAGE

Gluconacetobacter diazotrophicus

129000

-

nondenaturing PAGE

Gluconacetobacter diazotrophicus

-

Gluconacetobacter diazotrophicus

-70Â°C, stable for several months

Gluconacetobacter diazotrophicus

-70Â°C, stable for weeks without appreciable loss of activity

Gluconacetobacter diazotrophicus

4Â°C, stable for several weeks

Gluconacetobacter diazotrophicus

4Â°C, stable for several weeks without appreciable loss of activity

Gluconacetobacter diazotrophicus

acetaldehyde + 2 ferricyanide + H2O

-

731999

Gluconacetobacter diazotrophicus

acetate + 2 ferrocyanide + 2 H+

-

?

acetaldehyde + 2 ferricyanide + H2O

-

731999

Gluconacetobacter diazotrophicus ATCC 49037

acetate + 2 ferrocyanide + 2 H+

-

?

acetaldehyde + ferricyanide

-

731999

Gluconacetobacter diazotrophicus

acetic acid + ferrocyanide + H+

-

?

acetaldehyde + ferricyanide

-

731999

Gluconacetobacter diazotrophicus ATCC 49037

acetic acid + ferrocyanide + H+

-

?

acetaldehyde + oxidized phenazine methosulfate

-

731999

Gluconacetobacter diazotrophicus

acetic acid + reduced phenazine methosulfate

-

?

acetaldehyde + oxidized phenazine methosulfate

-

731999

Gluconacetobacter diazotrophicus ATCC 49037

acetic acid + reduced phenazine methosulfate

-

?

glutaraldehyde + 2 ferricyanide + H2O

26.4% compared to the activity with acetaldehyde

731999

Gluconacetobacter diazotrophicus

glutarate + 2 ferrocyanide + 2 H+

-

?

glutaraldehyde + oxidized phenazine methosulfate

26.5% of the activity with acetaldehyde

731999

Gluconacetobacter diazotrophicus

glutaric acid + reduced phenazine methosulfate

-

?

additional information

formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme

731999

Gluconacetobacter diazotrophicus

?

-

?

additional information

no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes

731999

Gluconacetobacter diazotrophicus

?

-

?

additional information

formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme

731999

Gluconacetobacter diazotrophicus ATCC 49037

?

-

?

additional information

no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes

731999

Gluconacetobacter diazotrophicus ATCC 49037

?

-

?

propanal + oxidized phenazine methosulfate

72.7% of the activity with acetaldehyde

731999

Gluconacetobacter diazotrophicus

propanoic acid + reduced phenazine methosulfate

-

?

propionaldehyde + 2 ferricyanide + H2O

72.7% compared to the activity with acetaldehyde

731999

Gluconacetobacter diazotrophicus

propionate + 2 ferrocyanide + 2 H+

-

?

heterodimer

1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions

Gluconacetobacter diazotrophicus

heterodimer

1 * 79700 and 1 * 50000,SDS-PAGE

Gluconacetobacter diazotrophicus

22000

-

acetic acid

cosubstrate ferricyandie, pH 3.5, 30Â°C

Gluconacetobacter diazotrophicus

3.5

-

electron accceptor ferricyanide

Gluconacetobacter diazotrophicus

3.5

-

using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors

Gluconacetobacter diazotrophicus

7

-

electron acceptor phenazine methosulfate plus 2,6-dichlorophenolindophenol

Gluconacetobacter diazotrophicus

7

-

using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors

Gluconacetobacter diazotrophicus

isoelectric focusing

-

5.2

6720

-

acetic acid

cosubstrate ferricyandie, pH 3.5, 30Â°C

Gluconacetobacter diazotrophicus

6720

-

acetic acid

cosubstrate ferricyandie, pH 3.5, 30Â°C

Gluconacetobacter diazotrophicus

731507

Aquino Neto

High current density PQQ-depen ...

Gluconobacter oxydans, Gluconobacter oxydans DSM 3504

Biosens. Bioelectron.

72

247-254

2015

-

1

-

3

-

1

-

731204

Zeiser

Characterization of a periplas ...

Sphingomonas wittichii, Sphingomonas wittichii DSM 6014

Appl. Microbiol. Biotechnol.

98

2067-2079

2014

-

1

-

4

1

-

8

-

9

-

9

-

15

2

1

-

1

-

1

-

1

-

4

-

1

-

8

-

9

-

15

2

1

-

1

-

1

-

1

-

1

724837

Aquino Neto

-

Direct electron transfer-based ...

Gluconobacter sp., Gluconobacter sp. 33 (DSM Z 3504)

Electrochim. Acta

87

323-329

2013

-

1

-

3

-

2

-

1

-

1

-

2

6

1

2

-

1

-

3

-

1

-

1

-

2

6

1

-

731035

Xu

-

Investigating the impact of mu ...

Gluconobacter oxydans, Gluconobacter oxydans DSM 3504

ACS Catal.

3

1756-1763

2013

-

1

-

3

-

2

-

1

-

731999

Gomez-Manzo

Molecular and catalytic proper ...

Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus ATCC 49037

J. Bacteriol.

192

5718-5724

2010

-

2

1

-

5

-

2

-

1

-

4

14

2

1

-

1

4

-

4

-

1

-

4

-

4

-

1

-

5

-

1

-

4

14

2

-

1

4

-

1

-

1

687166

Gomez-Manzo

The PQQ-alcohol dehydrogenase ...

Gluconacetobacter diazotrophicus

Int. J. Food Microbiol.

125

71-78

2008

-

1

-

3

1

-

1

-

1

-

1

-

5

1

-

1

-

1

-

2

-

1

-

2

-

1

-

3

1

-

1

-

1

-

5

1

-

1

-

1

-

1

-

685650

Shinagawa

A novel type of formaldehyde-o ...

Acetobacter sp., Acetobacter sp. SKU 14

Biosci. Biotechnol. Biochem.

70

850-857

2006

-

1

3

-

6

-

7

-

2

-

1

-

15

2

1

3

-

2

1

8

2

-

2

-

1

3

-

6

-

2

-

1

-

15

2

1

3

-

2

1

8

-

687366

Vangnai

Two distinct alcohol dehydroge ...

Thauera butanivorans

J. Bacteriol.

184

1916-1924

2002

1

-

1

-

1

-

1

-

1

-

1

-

3

1

2

-

2

-

1

-

1

2

-

1

-

1

-

1

-

3

1

-

389961

Zarnt

Catalytic and molecular proper ...

Cupriavidus necator, Cupriavidus necator Bo

J. Bacteriol.

183

1954-1960

2001

-

1

-

3

5

-

1

-

7

-

10

1

-

1

3

-

1

-

3

5

-

1

-

10

1

-

688853

Vangnai

An inducible 1-butanol dehydro ...

Thauera butanivorans

Microbiology

147

745-756

2001

2

-

1

2

-

1

-

1

-

1

4

1

10

1

-

1

-

2

-

1

-

2

-

2

-

2

-

1

2

-

1

4

1

10

1

-

2

-

1

-

684539

Zarnt

Degradation of tetrahydrofurfu ...

Cupriavidus necator

Appl. Environ. Microbiol.

63

4891-4898

1997

1

-

1

-

1

2

-

1

-

1

-

1

6

1

-

2

-

1

-

1

-

2

-

1

-

1

2

-

1

-

1

6

1

-

1

-

687362

Toyama

Three distinct quinoprotein al ...

Pseudomonas putida, Pseudomonas putida HK5

J. Bacteriol.

177

2442-2450

1995

3

-

1

-

3

-

6

-

9

-

3

-

3

4

-

28

3

4

-

3

-

6

-

3

-

3

-

6

-

1

-

3

-

6

-

3

-

3

4

-

28

3

-

3

-

3

-

389935

Görisch

Quinoprotein ethanol dehydroge ...

Pseudomonas aeruginosa, Pseudomonas putida

Antonie van Leeuwenhoek

56

35-45

1989

-

2

-

2

-

2

-

2

-

7

2

-

2

-

2

-

2

-

2

-

7

2

-

288470

Adachi

-

Preparation of cells and cytop ...

Acetobacter aceti

Agric. Biol. Chem.

52

2083-2084

1988

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

661296

Rupp

Purification, crystallisation ...

Pseudomonas aeruginosa

Biol. Chem. Hoppe-Seyler

369

431-439

1988

-

1

-

1

-

3

-

1

-

1

-

4

1

-

1

-

1

-

1

-

3

-

1

-

4

1

-

1

-

389953

Groen

Quinohaemoprotein alcohol dehy ...

Comamonas testosteroni

Biochem. J.

234

611-615

1986

-

1

-

1

-

1

-

1

5

-

2

-

2

-

1

-

1

-

1

5

-

288472

Hommel

-

A pyridine nucleotide-independ ...

Acetobacter pasteurianus

FEMS Microbiol. Lett.

22

139-142

1984

-

1

-

1

-

1

-

5

-

9

-

1

-

1

-

5

-

9

-

389951

Groen

Quinoprotein alcohol dehydroge ...

Pseudomonas aeruginosa

Biochem. J.

223

921-924

1984

-

3

-

1

-

1

-

3

-

1

-

1

-

3

-

1

-

3

-

288471

Ameyama

-

Aldehyde dehydrogenase from ac ...

Acetobacter aceti, Gluconobacter oxydans

Methods Enzymol.

89

491-497

1982

-

2

-

2

-

2

-

2

-

2

8

-

2

-

2

-

2

-

2

-

2

-

2

-

2

8

-

2

-

288474

Ameyama

-

Purification and characterizat ...

Acetobacter aceti

Agric. Biol. Chem.

45

1889-1890

1981

-

1

-

1

-

3

-

1

-

1

-

4

1

-

1

-

1

-

1

-

1

-

1

-

3

-

1

-

4

1

-

1

-

Molecular and catalytic properties of the aldehyde dehydrogenase of Gluconacetobacter diazotrophicus, a quinoheme protein containing pyrroloquinoline quinone, cytochrome b, and cytochrome c

Data extracted from this reference: