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Literature summary for 1.2.5.2 extracted from

  • Zeiser, J.; Muehlenbeck, L.H.; Schweiger, P.; Deppenmeier, U.
    Characterization of a periplasmic quinoprotein from Sphingomonas wittichii that functions as aldehyde dehydrogenase (2014), Appl. Microbiol. Biotechnol., 98, 2067-2079.
    View publication on PubMed
Search for more literature for 1.2.5.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhizorhabdus wittichii

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 10 mM, almost complete inhibition Rhizorhabdus wittichii
EDTA 1 mM, 25% decrease in activity Rhizorhabdus wittichii
FeCl3 10 mM, almost complete inhibition Rhizorhabdus wittichii
ZnSO4 10 mM, almost complete inhibition Rhizorhabdus wittichii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.3
-
 Butanal pH 8, 25°C Rhizorhabdus wittichii

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Rhizorhabdus wittichii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39300
-
 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer Rhizorhabdus wittichii
39300
-
 minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer Rhizorhabdus wittichii
39329
-
 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer Rhizorhabdus wittichii
39329
-
 minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer Rhizorhabdus wittichii
39400
-
 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer Rhizorhabdus wittichii
39400
-
 minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer Rhizorhabdus wittichii
330000
-
 PAGE, minor protein band Rhizorhabdus wittichii
450000
-
 PAGE Rhizorhabdus wittichii

Organism

Organism UniProt Comment Textmining
Rhizorhabdus wittichii A5VEL7
-
-
Rhizorhabdus wittichii DSM 6014 A5VEL7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
180
-
 substrate L-sorbosone, pH 8, 25°C Rhizorhabdus wittichii
1050
-
 substrate glyoxal, pH 8, 25°C Rhizorhabdus wittichii
1060
-
 substrate glycolaldehyde, pH 8, 25°C Rhizorhabdus wittichii
1170
-
 substrate 3-methylbenzaldehyde, pH 8, 25°C Rhizorhabdus wittichii
1450
-
 substrate valeraldehyde, pH 8, 25°C Rhizorhabdus wittichii
1690
-
 substrate methylglyoxal, pH 8, 25°C Rhizorhabdus wittichii
1740
-
 substrate propanal, pH 8, 25°C Rhizorhabdus wittichii
1900
-
 substrate phenylglyoxal, pH 8, 25°C Rhizorhabdus wittichii
2470
-
 substrate butanal, pH 8, 25°C Rhizorhabdus wittichii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylbenzaldehyde + ferricyanide
-
 Rhizorhabdus wittichii 3-methylbenzoic acid + ferrocyanide + H+
-
 ?
3-methylbenzaldehyde + ferricyanide
-
 Rhizorhabdus wittichii DSM 6014 3-methylbenzoic acid + ferrocyanide + H+
-
 ?
butanal + ferricyanide
-
 Rhizorhabdus wittichii butanoic acid + ferrocyanide + H+
-
 ?
butanal + ferricyanide
-
 Rhizorhabdus wittichii DSM 6014 butanoic acid + ferrocyanide + H+
-
 ?
glycolaldehyde + ferricyanide
-
 Rhizorhabdus wittichii hydroxyacetic acid + ferrocyanide + H+
-
 ?
glycolaldehyde + ferricyanide
-
 Rhizorhabdus wittichii DSM 6014 hydroxyacetic acid + ferrocyanide + H+
-
 ?
glyoxal + ferricyanide
-
 Rhizorhabdus wittichii oxoacetic acid + ferrocyanide + H+
-
 ?
glyoxal + ferricyanide
-
 Rhizorhabdus wittichii DSM 6014 oxoacetic acid + ferrocyanide + H+
-
 ?
L-sorbosone + ferricyanide
-
 Rhizorhabdus wittichii ? + ferrocyanide + H+
-
 ?
methylglyoxal + ferricyanide
-
 Rhizorhabdus wittichii 2-oxopropanoic acid + ferrocyanide + H+
-
 ?
additional information the enzyme can also transfer electrons to the electron acceptor system DCPIP/PMS, but the activity is only 50% of the rate obtained with ferricyanide. Enzyme displays a broad substrate spectrum and oxidizes short- and medium-chain aliphatic aldehydes (C1-C6), ketoaldehydes, dialdehydes; some aromatic aldehydes and hydroxyaldehydes to the corresponding carboxylic acids Rhizorhabdus wittichii ?
-
 ?
additional information the enzyme can also transfer electrons to the electron acceptor system DCPIP/PMS, but the activity is only 50% of the rate obtained with ferricyanide. Enzyme displays a broad substrate spectrum and oxidizes short- and medium-chain aliphatic aldehydes (C1-C6), ketoaldehydes, dialdehydes; some aromatic aldehydes and hydroxyaldehydes to the corresponding carboxylic acids Rhizorhabdus wittichii DSM 6014 ?
-
 ?
phenylglyoxal + ferricyanide
-
 Rhizorhabdus wittichii oxo(phenyl) acetic acid + ferrocyanide + H+
-
 ?
propanal + ferricyanide
-
 Rhizorhabdus wittichii propanoic acid + ferrocyanide + H+
-
 ?
valeraldehyde + ferricyanide
-
 Rhizorhabdus wittichii pentanoic acid + ferrocyanide + H+
-
 ?

Subunits

Subunits Comment Organism
dodecamer 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer Rhizorhabdus wittichii
octamer minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer Rhizorhabdus wittichii

Synonyms

Synonyms Comment Organism
glucose sorbosone dehydrogenase
-
 Rhizorhabdus wittichii
Swit_4395
-
 Rhizorhabdus wittichii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Rhizorhabdus wittichii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Rhizorhabdus wittichii

pH Stability

pH Stability pH Stability Maximum Comment Organism
9.5
-
 half-life below 5 min Rhizorhabdus wittichii

General Information

General Information Comment Organism
physiological function overproduction of enzyme Swit_4395 in Sphongomonas wittichii RW1 dramatically increases the tolerance of the bacterium toward butyraldehyde and thus might contribute to the detoxification of toxic aldehydes Rhizorhabdus wittichii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
43
-
 Butanal pH 8, 25°C Rhizorhabdus wittichii