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Literature summary for 1.2.5.2 extracted from

  • Aquino Neto, S.; Suda, E.; Xu, S.; Meredith, M.; De Andrade, A.; Minteer, S.
    Direct electron transfer-based bioanodes for ethanol biofuel cells using PQQ-dependent alcohol and aldehyde dehydrogenases (2013), Electrochim. Acta, 87, 323-329.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.86
-
acetaldehyde at pH 7.2 and 25°C Gluconobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
1 * 80000 + 1 * 50000, SDS-PAGE Gluconobacter sp.
80000
-
1 * 80000 + 1 * 50000, SDS-PAGE Gluconobacter sp.
141000
-
gel filtration Gluconobacter sp.

Organism

Organism UniProt Comment Textmining
Gluconobacter sp.
-
-
-
Gluconobacter sp. 33 (DSM Z 3504)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Toyo-Pearl 650M column chromatography and Sephadex G-100 gel filtration Gluconobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:D-mannitol-grown cell
-
Gluconobacter sp.
-

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 30 days, remains stable Gluconobacter sp.
4°C, purified enzyme, few days, remains stable Gluconobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + 2,6-dichlorophenol indophenol
-
Gluconobacter sp. acetate + reduced 2,6-dichlorophenol indophenol
-
?
acetaldehyde + 2,6-dichlorophenol indophenol
-
Gluconobacter sp. 33 (DSM Z 3504) acetate + reduced 2,6-dichlorophenol indophenol
-
?
acetaldehyde + 2,6-dichlorophenol indophenol
-
Gluconobacter sp. DSM 3504 / ATCC 15163 acetate + reduced 2,6-dichlorophenol indophenol
-
?
acetaldehyde + phenazine methosulfate
-
Gluconobacter sp. acetate + reduced phenazine methosulfate
-
?
acetaldehyde + phenazine methosulfate
-
Gluconobacter sp. 33 (DSM Z 3504) acetate + reduced phenazine methosulfate
-
?
acetaldehyde + phenazine methosulfate
-
Gluconobacter sp. DSM 3504 / ATCC 15163 acetate + reduced phenazine methosulfate
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 80000 + 1 * 50000, SDS-PAGE Gluconobacter sp.

Synonyms

Synonyms Comment Organism
PQQ-AldDH
-
Gluconobacter sp.
pyrroloquinoline quinone-dependent aldehyde dehydrogenase
-
Gluconobacter sp.