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Literature summary for 1.2.5.2 extracted from

  • Toyama, H.; Fujii, A.; Matsushita, K.; Shinagawa, E.; Ameyama, M.; Adachi, O.
    Three distinct quinoprotein alcohol dehydrogenases are expressed when Pseudomonas putida is grown on different alcohols (1995), J. Bacteriol., 177, 2442-2450.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.5.2

Activating Compound

Activating Compound Comment Organism Structure
ethylamine little ADH I activity is detected in the absence of ethylamine or ammonium ions Pseudomonas putida
additional information ADH I is induced by ethanol Pseudomonas putida
NH4+ little ADH I activity is detected in the absence of ethylamine or ammonium ions Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
EDTA allows dissociation of ADH I into two identical monomer subunits, releasing PQQ Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Pseudomonas putida
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 ADH IIB, gel filtration Pseudomonas putida
64000
-
 2 * 64000, ADH I, SDS-PAGE Pseudomonas putida
68000
-
 ADH IIG, gel filtration Pseudomonas putida
69000
-
 1 * 69000, ADH IIB Pseudomonas putida
72000
-
 1 * 72000, ADH IIG Pseudomonas putida
130000
-
 ADH I, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas putida three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol ?
-
 ?
additional information Pseudomonas putida three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length ?
-
 ?
additional information Pseudomonas putida three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol ?
-
 ?
additional information Pseudomonas putida HK5 three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol ?
-
 ?
additional information Pseudomonas putida HK5 three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol ?
-
 ?
additional information Pseudomonas putida HK5 three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 isozyme ADH I
-
Pseudomonas putida Q4W6G0 ADH IIG; isozyme ADH IIG
-
Pseudomonas putida Q8GR64 isozyme ADH IIB
-
Pseudomonas putida HK5
-
 isozyme ADH I
-
Pseudomonas putida HK5 Q4W6G0 ADH IIG; isozyme ADH IIG
-
Pseudomonas putida HK5 Q8GR64 isozyme ADH IIB
-

Purification (Commentary)

Purification (Comment) Organism
native isozyme ADH I, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview Pseudomonas putida
native isozyme ADH IIB, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview Pseudomonas putida
native isozyme ADH IIG, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:1,2-propanediol-grown cell isozyme ADH IIG Pseudomonas putida
-
culture condition:ethanol-grown cell isozyme ADH I Pseudomonas putida
-
culture condition:n-butanol-grown cell isozyme ADH IIB Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificities of ADH isozymes, detailed overview Pseudomonas putida
14
-
 purified ADH IIG, substrate glycerol with cofactor ferricyanide Pseudomonas putida
26.8
-
 purified ADH I, substrate ethanol with cofactors Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + 2 ferricyanide + H2O
-
 Pseudomonas putida acetate + 2 ferrocyanide + 2 H+
-
 ?
acetaldehyde + 2 ferricyanide + H2O
-
 Pseudomonas putida HK5 acetate + 2 ferrocyanide + 2 H+
-
 ?
acetaldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
acetaldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
acetaldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida HK5 acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
acetaldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida HK5 acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
butanal + 2 ferricyanide + H2O
-
 Pseudomonas putida butanoate + 2 ferrocyanide + 2 H+
-
 ?
butanal + 2 ferricyanide + H2O
-
 Pseudomonas putida HK5 butanoate + 2 ferrocyanide + 2 H+
-
 ?
butyraldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida butanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
butyraldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida butanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
butyraldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida HK5 butanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
butyraldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida HK5 butanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol Pseudomonas putida ?
-
 ?
additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length Pseudomonas putida ?
-
 ?
additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol Pseudomonas putida ?
-
 ?
additional information ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors Pseudomonas putida ?
-
 ?
additional information isozyme ADH IIG shows no activity with O2 Pseudomonas putida ?
-
 ?
additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol Pseudomonas putida HK5 ?
-
 ?
additional information ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors Pseudomonas putida HK5 ?
-
 ?
additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol Pseudomonas putida HK5 ?
-
 ?
additional information isozyme ADH IIG shows no activity with O2 Pseudomonas putida HK5 ?
-
 ?
additional information three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length Pseudomonas putida HK5 ?
-
 ?
propanal + 2 ferricyanide + H2O
-
 Pseudomonas putida propanoate + 2 ferrocyanide + 2 H+
-
 ?
propanal + 2 ferricyanide + H2O
-
 Pseudomonas putida HK5 propanoate + 2 ferrocyanide + 2 H+
-
 ?
propionaldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida propanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
propionaldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida propanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
propionaldehyde + 2,6-dichlorophenol indophenol in presence of phenazine methosulfate Pseudomonas putida HK5 propanoate + reduced 2,6-dichlorophenol indophenol
-
 ?
propionaldehyde + 2,6-dichlorophenol indophenol with phenazine methosulfonate Pseudomonas putida HK5 propanoate + reduced 2,6-dichlorophenol indophenol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 64000, ADH I, SDS-PAGE Pseudomonas putida
monomer 1 * 69000, ADH IIB Pseudomonas putida
monomer 1 * 72000, ADH IIG Pseudomonas putida

Synonyms

Synonyms Comment Organism
ADH I
-
 Pseudomonas putida
ADH IIB
-
 Pseudomonas putida
ADH IIG
-
 Pseudomonas putida
quinohemoprotein dehydrogenase
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 about, ADH IIB Pseudomonas putida
8
-
 about, ADH IIG Pseudomonas putida
9
-
 about, ADH I Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
heme c isozyme ADH IIB is a quinohemoprotein dehydrogenase, determination of heme content, overview Pseudomonas putida
heme c isozymes ADH IIB and ADH IIG are quinohemoprotein dehydrogenases, while ADH I is a non-heme quinoprotein, determination of heme contents, overview Pseudomonas putida
additional information ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors Pseudomonas putida
pyrroloquinoline quinone PQQ, a prosthetic group, ADH I is a non-heme quinoprotein containing 2.2 mol of PQQ per mol of enzyme Pseudomonas putida
pyrroloquinoline quinone PQQ, a prosthetic group, isozyme ADH IIB is a quinohemoprotein dehydrogenase. ADH IIB contains 1.4 mol of PQQ per mol of enzyme on the basis of a molecular weight of 70000 Da Pseudomonas putida
pyrroloquinoline quinone PQQ, a prosthetic group, isozyme ADH IIG is a quinohemoprotein dehydrogenase. ADH IIG contains 1.2 mol of PQQ per mol of enzyme on the basis of a molecular weight of 70000 Da Pseudomonas putida

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas putida ADH IIG, isoelectric focusing
-
 5
Pseudomonas putida ADH IIB, isoelectric focusing
-
 6.5
Pseudomonas putida ADH I, isoelectric focusing
-
 7.6