KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km value for formaldehyde is measured to be 1013 mM using the formaldehyde solution containing methanol. 2325 mM is obtained with freshly prepared formaldehyde solution only with distilled water. A Km value over 50 mM is observed when using the substrate solution stored in a refrigerator over one month | Acetobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Acetobacter sp. | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
1 * 18000 + 1 * 55000 + 1 * 78000, SDS-PAGE | Acetobacter sp. |
18000 | - |
x * 78000 + x * 55000 + x * 18000, SDS-PAGE | Acetobacter sp. |
55000 | - |
1 * 18000 + 1 * 55000 + 1 * 78000, SDS-PAGE | Acetobacter sp. |
55000 | - |
x * 78000 + x * 55000 + x * 18000, SDS-PAGE | Acetobacter sp. |
78000 | - |
1 * 18000 + 1 * 55000 + 1 * 78000, SDS-PAGE | Acetobacter sp. |
78000 | - |
x * 78000 + x * 55000 + x * 18000, SDS-PAGE | Acetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter sp. | - |
SKU 14 | - |
Acetobacter sp. | - |
isolated in Thailand | - |
Acetobacter sp. SKU 14 | - |
SKU 14 | - |
Acetobacter sp. SKU 14 | - |
isolated in Thailand | - |
Purification (Comment) | Organism |
---|---|
- |
Acetobacter sp. |
native enzyme 130fold from membranes of glycerol-grown cells by two different steps of anion exchange chromatography, solubilization with 0.1% Triton X-100 or Tween 20, copurification with a cytochrome c | Acetobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:glycerol-grown cell | - |
Acetobacter sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
293 | - |
purified native enzyme | Acetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + 2 ferricyanide + H2O | - |
Acetobacter sp. | acetate + 2 ferrocyanide + 2 H+ | - |
? | |
acetaldehyde + 2 ferricyanide + H2O | about 30% of the activity with formaldehyde | Acetobacter sp. | acetate + 2 ferrocyanide + 2 H+ | - |
? | |
acetaldehyde + 2 ferricyanide + H2O | - |
Acetobacter sp. SKU 14 | acetate + 2 ferrocyanide + 2 H+ | - |
? | |
acetaldehyde + 2 ferricyanide + H2O | about 30% of the activity with formaldehyde | Acetobacter sp. SKU 14 | acetate + 2 ferrocyanide + 2 H+ | - |
? | |
butanal + 2 ferricyanide + H2O | - |
Acetobacter sp. | butanoate + 2 ferrocyanide + 2 H+ | - |
? | |
butanal + 2 ferricyanide + H2O | about 30% of the activity with formaldehyde | Acetobacter sp. | butanoate + 2 ferrocyanide + 2 H+ | - |
? | |
butanal + 2 ferricyanide + H2O | - |
Acetobacter sp. SKU 14 | butanoate + 2 ferrocyanide + 2 H+ | - |
? | |
butanal + 2 ferricyanide + H2O | about 30% of the activity with formaldehyde | Acetobacter sp. SKU 14 | butanoate + 2 ferrocyanide + 2 H+ | - |
? | |
formaldehyde + 2 ferricyanide + H2O | - |
Acetobacter sp. | formate + 2 ferrocyanide + 2 H+ | - |
? | |
glutaraldehyde + 4 ferricyanide + 2 H2O | - |
Acetobacter sp. | glutarate + 4 ferrocyanide + 4 H+ | - |
? | |
glutaraldehyde + 4 ferricyanide + 2 H2O | about 40% of the activity with formaldehyde | Acetobacter sp. | glutarate + 4 ferrocyanide + 4 H+ | - |
? | |
additional information | the substrate specificity of the enzyme is broad toward aldehydes and alcohols | Acetobacter sp. | ? | - |
? | |
additional information | the substrate specificity of the enzyme is broad toward aldehydes and alcohols | Acetobacter sp. SKU 14 | ? | - |
? | |
propanal + 2 ferricyanide + H2O | - |
Acetobacter sp. | propanoate + 2 ferrocyanide + 2 H+ | - |
? | |
propanal + 2 ferricyanide + H2O | about 40% of the activity with formaldehyde | Acetobacter sp. | propanoate + 2 ferrocyanide + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000 + x * 55000 + x * 18000, SDS-PAGE | Acetobacter sp. |
trimer | 1 * 18000 + 1 * 55000 + 1 * 78000, SDS-PAGE | Acetobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
FOE | - |
Acetobacter sp. |
formaldehyde-oxidizing enzyme | - |
Acetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Acetobacter sp. |
40 | - |
substrate: formaldehyde | Acetobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 75% of maximal activity, 50°C: about 60% of maximal activity | Acetobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min, stable | Acetobacter sp. |
45 | - |
10 min, about 50% loss of activity | Acetobacter sp. |
50 | - |
10 min, 95% loss of activity | Acetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Acetobacter sp. |
5 | 6 | substrate: formaldehyde | Acetobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5-6: optimum, pH 9: about 60% of maximal activity | Acetobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
overnight, at 6°C, about 20% loss of activity | Acetobacter sp. |
3 | 9 | stable at 6°C, overnight | Acetobacter sp. |
5 | 7 | overnight, at 6°C, stable | Acetobacter sp. |
9 | - |
overnight, at 6°C, about 20% loss of activity | Acetobacter sp. |
10 | - |
at 6°C, overnight, about 75% inactivation | Acetobacter sp. |
10 | - |
overnight, at 6°C, about 60% loss of activity | Acetobacter sp. |
11 | - |
at 6°C, overnight, complete inactivation | Acetobacter sp. |
11 | - |
overnight, at 6°C, complete loss of activity | Acetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the two subunits of 78000 Da and 55000 Da contain cytochrome c | Acetobacter sp. | |
pyrroloquinoline quinone | - |
Acetobacter sp. |