Activating Compound | Comment | Organism | Structure |
---|---|---|---|
lauric acid | activation by covalent attachment, binding site Lys544 | Escherichia coli | |
Lipids | enzyme is activated by lipids, high affinity binding site | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain CG3 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | peripheral membrane-associated enzyme | Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | divalent metal ion required | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
the lauric acid-labeled enzyme is not digested neither by trypsin nor alpha-chymotrypsin in the presence of 0.1% SDS. Effective digestion is achieved by thermolysin, to a 45000 and a 15000 Da fragment | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli CG3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the enzyme is purified from an Escherichia coli strain CG3 harboring a plasmid carrying a plasmid th eoxidase gene | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + ferricyanide + H2O | addition of 1% lauric acid | Escherichia coli | acetate + CO2 + ferrocyanide | - |
? | |
pyruvate + ferricyanide + H2O | addition of 1% lauric acid | Escherichia coli CG3 | acetate + CO2 + ferrocyanide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pyruvate oxidase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | required | Escherichia coli |