Literature summary for 1.2.5.1 extracted from

Recny, M.A.; Hager, L.P.
Reconstitution of native Escherichia coli pyruvate oxidase from apoenzyme monomers and FAD (1982), J. Biol. Chem., 257, 12878-12886.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell membrane	cell membrane-associated	Escherichia coli	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pyruvate + ferricytochrome b1 + H2O	Escherichia coli	-	acetate + CO2 + ferrocytochrome b1	-	?
pyruvate + ubiquinone-6 + H2O	Escherichia coli	-	acetate + CO2 + ubiquinol-6	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
reconstitution of the native enzymatically active protein can be accomplished by incubating equimolar concentrations of apomonomers and FAD at pH 6.5. The second order reaction of apomonomers with FAD to form an initial monomer-FAD complex is fast. The rate-limiting step for enzymatic reactivation appears to be the folding of the polypeptide chain in the monomer-FAD complex to reconstitute the three-dimensional FAD binding site prior to subunit reassociation. The subsequent formation of native tetramers proceeds via an essentially irreversible dimer assembly pathway	Escherichia coli

Renatured (Comment)

Organism

reconstitution of the native enzymatically active protein can be accomplished by incubating equimolar concentrations of apomonomers and FAD at pH 6.5. The second order reaction of apomonomers with FAD to form an initial monomer-FAD complex is fast. The rate-limiting step for enzymatic reactivation appears to be the folding of the polypeptide chain in the monomer-FAD complex to reconstitute the three-dimensional FAD binding site prior to subunit reassociation. The subsequent formation of native tetramers proceeds via an essentially irreversible dimer assembly pathway

Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
pyruvate + ferricyanide + H2O	-	Escherichia coli	acetate + CO2 + ferrocyanide	-	?
pyruvate + ferricytochrome b1 + H2O	-	Escherichia coli	acetate + CO2 + ferrocytochrome b1	-	?
pyruvate + ubiquinone-6 + H2O	-	Escherichia coli	acetate + CO2 + ubiquinol-6	-	?

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Escherichia coli