BRENDA - Enzyme Database show
show all sequences of 1.2.5.1

Reconstitution of the lipid-depleted pyruvate oxidase system of Escherichia coli: the palmitic acid effect

Kiuchi, K.; Hager, L.P.; Arch. Biochem. Biophys. 233, 776-784 (1984)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
cis-12-hydroxy-9-octadecenoic acid
119% of the activitation with palmitic acid
Escherichia coli
elaidic acid
122% of the activitation with palmitic acid
Escherichia coli
lauric acid
126% of the activitation with palmitic acid
Escherichia coli
linoleic acid
116% of the activitation with palmitic acid
Escherichia coli
linolelaidic acid
113% of the activitation with palmitic acid
Escherichia coli
linolenic acid
126% of the activitation with palmitic acid
Escherichia coli
myristic acid
109% of the activitation with palmitic acid
Escherichia coli
n-nonanoic acid
42% of the activitation with palmitic acid
Escherichia coli
oleic acid
102% of the activitation with palmitic acid
Escherichia coli
palmitic acid
activation
Escherichia coli
palmitoleic acid
137% of the activitation with palmitic acid
Escherichia coli
trans-12-hydroxy-9-octadecenoic acid
103% of the activitation with palmitic acid
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyruvate + ubiquinone-30 + H2O
ubiquinone-30 is rapidly reduced by pyruvate oxidase only in the presence of palmitic acid
701890
Escherichia coli
acetate + CO2 + ubiquinol-30
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
cis-12-hydroxy-9-octadecenoic acid
119% of the activitation with palmitic acid
Escherichia coli
elaidic acid
122% of the activitation with palmitic acid
Escherichia coli
lauric acid
126% of the activitation with palmitic acid
Escherichia coli
linoleic acid
116% of the activitation with palmitic acid
Escherichia coli
linolelaidic acid
113% of the activitation with palmitic acid
Escherichia coli
linolenic acid
126% of the activitation with palmitic acid
Escherichia coli
myristic acid
109% of the activitation with palmitic acid
Escherichia coli
n-nonanoic acid
42% of the activitation with palmitic acid
Escherichia coli
oleic acid
102% of the activitation with palmitic acid
Escherichia coli
palmitic acid
activation
Escherichia coli
palmitoleic acid
137% of the activitation with palmitic acid
Escherichia coli
trans-12-hydroxy-9-octadecenoic acid
103% of the activitation with palmitic acid
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyruvate + ubiquinone-30 + H2O
ubiquinone-30 is rapidly reduced by pyruvate oxidase only in the presence of palmitic acid
701890
Escherichia coli
acetate + CO2 + ubiquinol-30
-
-
-
?
Other publictions for EC 1.2.5.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741260
Ianniello
Investigation of factors affec ...
Lactobacillus casei, Lactobacillus casei N87
PLoS ONE
11
e0164065
2016
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739946
Ma
Anaerobic humus and Fe(III) re ...
Thauera humireducens, Thauera humireducens SgZ-1
Appl. Microbiol. Biotechnol.
99
3619-3628
2015
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740232
Endo
Comparative genomics of Fructo ...
no activity in Fructobacillus
BMC Genomics
16
1117
2015
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740357
Borisov
Oxygen as acceptor ...
Escherichia coli
EcoSal Plus
6
0000
2015
-
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1
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3
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741413
Goris
Proteomics of the organohalide ...
Sulfurospirillum multivorans
Sci. Rep.
5
13794
2015
-
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1
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740554
An
Desulfocarbo indianensis gen. ...
no activity in Desulfocarbo indianensis
Int. J. Syst. Evol. Microbiol.
64
2907-2914
2014
-
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1
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740960
Li
-
The metabolic potential of Esc ...
Escherichia coli
Microb. Cell Fact.
13
0000
2014
-
-
1
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741301
Steinsiek
Analysis of Escherichia coli m ...
Escherichia coli
PLoS ONE
9
e87307
2014
-
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1
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1
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1
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1
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1
-
-
724036
Buchholz
Platform Engineering: Coryneba ...
Corynebacterium glutamicum, Corynebacterium glutamicum aceE A16
Appl. Environ. Microbiol.
79
5566-5575
2013
-
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2
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3
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2
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-
1
1
-
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-
724069
Wieschalka
Engineering Corynebacterium gl ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Appl. Microbiol. Biotechnol.
94
449-459
2012
-
-
-
-
-
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-
1
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2
-
25
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2
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2
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1
1
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700962
Neumann
Structural basis for membrane ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
105
17390-17395
2008
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704267
Schreiner
Pyruvate:quinone oxidoreductas ...
Corynebacterium glutamicum
J. Bacteriol.
188
1341-1350
2006
-
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1
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4
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2
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1
1
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702204
Marchal
Rate constants in two dimensio ...
Escherichia coli
Biochemistry
40
1248-1256
2001
-
-
-
-
-
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1
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2
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1
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1
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1
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1
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1
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1
-
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1
1
-
-
-
702194
Chang
Sulfhydryl chemistry detects t ...
Escherichia coli
Biochemistry
36
11564-11573
1997
-
-
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2
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1
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1
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701892
Bertagnolli
Role of flavin in acetoin prod ...
Escherichia coli, Pediococcus pseudomonas
Arch. Biochem. Biophys.
300
364-371
1993
-
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5
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1
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1
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704375
Bertagnolli
Activation of Escherichia coli ...
Escherichia coli
J. Biol. Chem.
266
10168-10173
1991
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1
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704376
Wang
Role of the tetrameric structu ...
Escherichia coli
J. Biol. Chem.
266
10959-10966
1991
-
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9
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1
2
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1
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1
2
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1
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1
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9
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1
2
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1
2
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702186
Grabau
In vivo function of Escherichi ...
Escherichia coli
Biochemistry
25
3748-3751
1986
-
-
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1
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1
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1
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1
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1
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705954
Grabau
Nucleotide sequence and deduce ...
Escherichia coli
Nucleic Acids Res.
14
5449-5460
1986
-
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1
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1
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1
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1
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707462
Hamilton
Identification of the high-aff ...
Escherichia coli, Escherichia coli CG3
Biochemistry
25
8179-8183
1986
2
-
1
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1
1
1
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2
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1
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2
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1
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1
1
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1
1
1
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1
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2
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704373
Carter
Reconstitution of the Ubiquino ...
Escherichia coli
J. Biol. Chem.
260
10986-10990
1985
-
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2
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1
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1
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1
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1
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1
1
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704374
Mather
Kinetic studies of the lipid-a ...
Escherichia coli
J. Biol. Chem.
260
16148-16155
1985
-
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1
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1
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1
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-
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-
-
701890
Kiuchi
Reconstitution of the lipid-de ...
Escherichia coli
Arch. Biochem. Biophys.
233
776-784
1984
12
-
-
-
-
-
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1
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1
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12
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1
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-
702185
Koland
Reconstitution of the membrane ...
Escherichia coli
Biochemistry
23
445-453
1984
-
-
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2
-
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1
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2
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1
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1
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1
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1
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1
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