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Literature summary for 1.2.5.1 extracted from
- Crystalline pyruvate oxidase from Escherichia coli. II. Activation by phospholipids (1971), J. Biol. Chem., 246, 1575-1582.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| lecithin | the hydrophobic moieties of lecithin activate pyruvate oxidase whereas the hydrophilic portions of the molecule have no stimulatory effect | Escherichia coli |  |
| lysophosphatidylethanolamine | highest stimulating activity among the phospholipid extracted from cell membranes tested, if the phospholipids are added directly to the assay mixtures. When water-soluble micellar preparations are substituted for direct addition of the phospholipid to the assay, all the phosphatides demonstrate higher specific activities for stimulating pyruvate oxidase, and the differences in their stimulating capacity are minimized | Escherichia coli | |
| additional information | stimlating effect of phopholipids, if added directly to the assay mixtures. When water-soluble micellar preparations are substituted for direct addition of the phospholipid to the assay, all the phosphatides demonstrate higher specific activities for stimulating pyruvate oxidase. The differences originally noted in the activating capacities of the various cell envelope phospholipids are minimized. The Km values for the cell envelope phospholipids, synthetic phosphatidylethanolamine, lecithin, and lysolecithin range from 0.9 to 2.2 microM. The Km value for phosphatidylserine is 6.5 microM. The diacylphospholipids exhibit normal Michaelis-Menten kinetics. Lysophosphatides demonstrate considerable divergence from normal Michaelis-Menten kinetics | Escherichia coli | |
| oleic acid | - |
Escherichia coli | |
| palmitic acid | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sephadex, ammonium sulfate precipitation, dialysis | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + oxidized 2,6-dichloroindophenol + H2O | - |
Escherichia coli | acetate + CO2 + reduced 2,6-dichloroindophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pyruvate oxidase | - |
Escherichia coli |
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