Crystallization (Comment) | Organism |
---|---|
the crystalline enzyme does not contain thiamine diphosphate but has an absolute thiamine diphosphate requirement for the reduction of the enzyme-bound FAD | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
265000 | - |
determined by the Archibald method | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme, after crystallization, 6000 Units/mg protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + ferricyanide + H2O | - |
Escherichia coli | acetate + CO2 + ferrocyanide | - |
? | |
pyruvate + ferricytochrome b1 + H2O | - |
Escherichia coli | acetate + CO2 + ferrocytochrome b1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pyruvate oxidase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
ferricyanide | without activation of the flavoprotein by proteolytic reactions, i.e. trypsin or chymotrypsin, pH and temperature not specified in the publication | Escherichia coli | |
167 | - |
pyruvate | pH and temperature not specified in the publication | Escherichia coli | |
883 | - |
ferricyanide | with activation of the flavoprotein by proteolytic reactions, i.e. trypsin or chymotrypsin, pH and temperature not specified in the publication | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli |