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Literature summary for 1.2.4.4 extracted from
- Purification of branched-chain keto acid dehydrogenase regulator from Pseudomonas putida (2000), Methods Enzymol., 324, 329-335.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain DH5alpha | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19850 | - |
4 * 19850, gel filtration | Pseudomonas putida |
| 20000 | - |
4 * 20000, SDS-PAGE | Pseudomonas putida |
| 79400 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose CL-6B column chromatography and heparin-Sepharose CL-6B column chromatography | Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 10 mM Tris-HC1, pH 8.0, containing 0.01 mM EDTA, 0.2 M NaCI, 0.005% (v/v) 2-mercaptoethanol, and 50% (v/v) glycerol, 2 years, remains active | Pseudomonas putida |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 20000, SDS-PAGE | Pseudomonas putida |
| homotetramer | 4 * 19850, gel filtration | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bkd | - |
Pseudomonas putida |
| branched-chain keto acid dehydrogenase | contains three components: E1 (alpha2/beta2, dehydrogenase-decarboxylase), E2 (transacylase), and E3 (lipoamide dehydrogenase) | Pseudomonas putida |
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