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Literature summary for 1.2.4.4 extracted from

  • Li, L.; Thipyapong, P.; Breeden, D.C.; Steffens, J.C.
    Overexpression of a bacterial branched-chain alpha-keto acid dehydrogenase complex in Arabidopsis results in accumulation of branched-chain acyl-CoAs and alteration of free amino acid composition in seeds (2003), Plant Sci., 165, 1213-1219.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of bacterial branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKDC, encoded by 4 genes, in Arabidopsis thaliana targeted to chloroplasts, about 7fold increased enzyme activity in the transgenic plants Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic Arabidopsis thaliana plants overexpressing the branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKDC in chloroplasts via Agrobacterium tumefaciens transformation under control of the CaMV 35S promotor, accumulation of branched-chain acyl-CoAs and alteration of free amino acid composition in seeds, overview Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-isocaproate + [dihydrolipoyllysine-residue(2-methylpropanoyl)transferase]-lipoyllysine overall reaction of the recombinant branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKADH Pseudomonas putida ?
-
?
2-oxoisovalerate + [dihydrolipoyllysine-residue(2-methylpropanoyl)transferase]-lipoyllysine overall reaction of the recombinant branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKADH Pseudomonas putida ?
-
?

Synonyms

Synonyms Comment Organism
branched-chain alpha-keto acid dehydrogenase
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Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudomonas putida