Cloned (Comment) | Organism |
---|---|
overexpression of bacterial branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKDC, encoded by 4 genes, in Arabidopsis thaliana targeted to chloroplasts, about 7fold increased enzyme activity in the transgenic plants | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Arabidopsis thaliana plants overexpressing the branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKDC in chloroplasts via Agrobacterium tumefaciens transformation under control of the CaMV 35S promotor, accumulation of branched-chain acyl-CoAs and alteration of free amino acid composition in seeds, overview | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-isocaproate + [dihydrolipoyllysine-residue(2-methylpropanoyl)transferase]-lipoyllysine | overall reaction of the recombinant branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKADH | Pseudomonas putida | ? | - |
? | |
2-oxoisovalerate + [dihydrolipoyllysine-residue(2-methylpropanoyl)transferase]-lipoyllysine | overall reaction of the recombinant branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKADH | Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
branched-chain alpha-keto acid dehydrogenase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas putida |