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Literature summary for 1.2.4.4 extracted from

  • Hawes, J.W.; Schnepf, R.J.; Jenkins, A.E.; Shimomura, Y.; Popov, K.M.; Harris, R.A.
    Roles of amino acid residues surrounding phosphorylation site 1 and branched-chain alpha-ketoacid dehydrogenase (BCKDH) in catalysis and phosphorylation site recognition by BCKDH kinase (1995), J. Biol. Chem., 270, 31071-31076.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
thiamine diphosphate
-
Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D296A inactive enzyme, no ability of component E1 apoenzyme to reconstitute with thiamine diphosphate Rattus norvegicus
H292A inactive enzyme, no binding of thiamine diphosphate Rattus norvegicus
R288A inactive enzyme, no detectable phosphorylation Rattus norvegicus
S293A 10fold increased Km-value for 3-methyl-2-oxobutanoate, 2.8fold increased Km-value for 4-methyl-2-oxopentanoate, no change in Km-value for thiamine diphosphate Rattus norvegicus
S293E no ability of the E1 apoenzyme to reconstitute with thiamine diphosphate Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
3-methyl-2-oxobutanoate wild-type enzyme and mutant enzyme D297A Rattus norvegicus
0.058
-
3-methyl-2-oxobutanoate mutant enzyme T294A Rattus norvegicus
0.059
-
3-methyl-2-oxobutanoate mutant enzyme G290A and S298A Rattus norvegicus
0.062
-
3-methyl-2-oxobutanoate mutant enzyme I289A and H291A Rattus norvegicus
0.064
-
3-methyl-2-oxobutanoate mutant enzyme S295A Rattus norvegicus
0.568
-
3-methyl-2-oxobutanoate mutant enzyme S293A Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine
-
Rattus norvegicus [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2
-
?
4-methyl-2-oxopentanoate + NAD+ + CoA
-
Rattus norvegicus 3-methylbutanoyl-CoA + CO2 + NADH
-
?

Cofactor

Cofactor Comment Organism Structure
CoA
-
Rattus norvegicus
NAD+
-
Rattus norvegicus