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Literature summary for 1.2.4.4 extracted from

  • Sinclair, D.A.; Dawes, I.W.; Dickinson, J.R.
    Purification and characterization of the branched chain alpha-ketoacid dehydrogenase complex from Saccharomyces cerevisiae (1993), Biochem. Mol. Biol. Int., 31, 911-922.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-Val no activation Saccharomyces cerevisiae
thiamine diphosphate
-
Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
ATP no inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
2-oxobutanoate
-
Saccharomyces cerevisiae
0.0133
-
2-oxo-4-methylthiobutanoate
-
Saccharomyces cerevisiae
0.0199
-
3-methyl-2-oxopentanoate
-
Saccharomyces cerevisiae
0.0218
-
4-methyl-2-oxopentanoate
-
Saccharomyces cerevisiae
0.0353
-
NAD+
-
Saccharomyces cerevisiae
0.213
-
3-methyl-2-oxobutanoate
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
alphax,betax, x * 478000 + x * 38000, SDS-PAGE Saccharomyces cerevisiae
478000
-
alphax,betax, x * 478000 + x * 38000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.82
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-70°C, 50 mM potassium phosphate buffer, pH 7.4, 0.1 mM EDTA, 0.2 mM thiamine diphosphate, 0.2 mM phenylmethylsulfonyl fluoride, stable for at least 3 weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-4-methylthiobutanoate + NAD+ + CoA
-
Saccharomyces cerevisiae 3-methylthiopropionyl-CoA + NADH + CO2
-
?
2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine 2-oxobutanoate Saccharomyces cerevisiae [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(propanoyl)dihydrolipoyllysine + CO2
-
?
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine
-
Saccharomyces cerevisiae [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2
-
?
3-methyl-2-oxopentanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine
-
Saccharomyces cerevisiae [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylbutanoyl)dihydrolipoyllysine + CO2
-
?
4-methyl-2-oxopentanoate + NAD+ + CoA
-
Saccharomyces cerevisiae 3-methylbutanoyl-CoA + CO2 + NADH
-
?

Subunits

Subunits Comment Organism
? alphax,betax, x * 478000 + x * 38000, SDS-PAGE Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
CoA
-
Saccharomyces cerevisiae
NAD+
-
Saccharomyces cerevisiae