Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | Km: 0.0012 mM in the reaction with 3-methyl-2-oxobutanoate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isopentanoyl-CoA | - |
Rattus norvegicus | |
NADH | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0105 | - |
DL-3-methyl-2-oxopentanoate | - |
Rattus norvegicus | |
0.013 | - |
3-methyl-2-oxobutanoate | - |
Rattus norvegicus | |
0.0146 | - |
4-methyl-2-oxopentanoate | - |
Rattus norvegicus | |
0.0387 | - |
NAD+ | reaction with DL-3-methyl-2-oxopentanoate | Rattus norvegicus | |
0.0395 | - |
NAD+ | reaction with 3-methyl-2-oxobutanoate | Rattus norvegicus | |
0.0423 | - |
NAD+ | reaction with 4-methyl-2-oxopentanoate | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
multienzyme complex | Rattus norvegicus |
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | - |
Rattus norvegicus | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 | - |
? | |
3-methyl-2-oxopentanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | - |
Rattus norvegicus | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylbutanoyl)dihydrolipoyllysine + CO2 | - |
? | |
4-methyl-2-oxopentanoate + NAD+ + CoA | - |
Rattus norvegicus | 3-methylbutanoyl-CoA + CO2 + NADH | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Rattus norvegicus | |
NAD+ | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
isovaleryl-CoA | reaction with 3-methyl-2-oxobutanoate | Rattus norvegicus | |
0.0101 | - |
isovaleryl-CoA | reaction with DL-3-methyl-2-oxopentanoate | Rattus norvegicus | |
0.0136 | - |
isovaleryl-CoA | reaction with 4-methyl-2-oxopentanoate | Rattus norvegicus | |
0.0177 | - |
NADH | reaction with 3-methyl-2-oxobutanoate | Rattus norvegicus | |
0.018 | - |
NADH | reaction with 4-methyl-2-oxopentanoate | Rattus norvegicus | |
0.023 | - |
NADH | reaction with DL-3-methyl-2-oxopentanoate | Rattus norvegicus |