Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.4.4 extracted from

  • Shimomura, Y.; Paxton, R.; Ozawa, T.; Harris, R.A.
    Purification of branched chain alpha-ketoacid dehydrogenase complex from rat liver (1987), Anal. Biochem., 163, 74-78.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
thiamine diphosphate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0215
-
4-methyl-2-oxopentanoate
-
Rattus norvegicus
0.0219
-
3-methyl-2-oxopentanoate
-
Rattus norvegicus
0.0372
-
3-methyl-2-oxobutanoate
-
Rattus norvegicus
0.97
-
pyruvate
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
alphax,betax, x * 46500 + x * 38500, SDS-PAGE Rattus norvegicus
46500
-
alphax,betax, x * 46500 + x * 38500, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hydrophobic interaction chromatography on phenyl-Sepharose Rattus norvegicus
multienzyme complex Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.46
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine
-
Rattus norvegicus [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2
-
?
3-methyl-2-oxopentanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine
-
Rattus norvegicus [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylbutanoyl)dihydrolipoyllysine + CO2
-
?
4-methyl-2-oxopentanoate + NAD+ + CoA
-
Rattus norvegicus 3-methylbutanoyl-CoA + CO2 + NADH
-
?
pyruvate + NAD+ + CoA
-
Rattus norvegicus acetyl-CoA + CO2 + NADH
-
?

Subunits

Subunits Comment Organism
? alphax,betax, x * 46500 + x * 38500, SDS-PAGE Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
CoA
-
Rattus norvegicus
NAD+
-
Rattus norvegicus