Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-Val | activates, when ommited from the assay mixture the rate of NAD+ reduction decreases to 41% of the rate of the complete system | Pseudomonas putida | |
thiamine diphosphate | required | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
coenzyme A | - |
Pseudomonas putida | |
0.12 | - |
NAD+ | reaction with 3-methyl-2-oxobutanoate | Pseudomonas putida | |
0.46 | - |
3-methyl-2-oxobutanoate | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
multienzyme complex | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.1 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
0-5°C, rapid loss of 20-30% of the activity, then stable for 3-4 months | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NAD+ + CoA | no activity | Pseudomonas putida | 3-carboxypropionyl-CoA + NADH + CO2 | - |
? | |
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | - |
Pseudomonas putida | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 | - |
? | |
3-methyl-2-oxopentanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine | - |
Pseudomonas putida | [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylbutanoyl)dihydrolipoyllysine + CO2 | - |
? | |
4-methyl-2-oxopentanoate + NAD+ + CoA | - |
Pseudomonas putida | 3-methylbutanoyl-CoA + CO2 + NADH | - |
? | |
pyruvate + NAD+ + CoA | no activity | Pseudomonas putida | acetyl-CoA + CO2 + NADH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Pseudomonas putida | |
NAD+ | - |
Pseudomonas putida |