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Literature summary for 1.2.4.4 extracted from

  • Sokatch, J.R.; McCully, V.; Roberts, C.M.
    Purification of a branched-chain keto acid dehydrogenase from Pseudomonas putida (1981), J. Bacteriol., 148, 647-652.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
L-Val activates, when ommited from the assay mixture the rate of NAD+ reduction decreases to 41% of the rate of the complete system Pseudomonas putida
thiamine diphosphate required Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
coenzyme A
-
Pseudomonas putida
0.12
-
NAD+ reaction with 3-methyl-2-oxobutanoate Pseudomonas putida
0.46
-
3-methyl-2-oxobutanoate
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
multienzyme complex Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.1
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
0-5°C, rapid loss of 20-30% of the activity, then stable for 3-4 months Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NAD+ + CoA no activity Pseudomonas putida 3-carboxypropionyl-CoA + NADH + CO2
-
?
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine
-
Pseudomonas putida [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2
-
?
3-methyl-2-oxopentanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine
-
Pseudomonas putida [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylbutanoyl)dihydrolipoyllysine + CO2
-
?
4-methyl-2-oxopentanoate + NAD+ + CoA
-
Pseudomonas putida 3-methylbutanoyl-CoA + CO2 + NADH
-
?
pyruvate + NAD+ + CoA no activity Pseudomonas putida acetyl-CoA + CO2 + NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
CoA
-
Pseudomonas putida
NAD+
-
Pseudomonas putida