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Literature summary for 1.2.4.4 extracted from

  • Parker, P.J.; Randle, P.J.
    Partial purification and properties of branched-chain 2-oxo acid dehydrogenase of ox liver (1978), Biochem. J., 171, 751-757.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
thiamine diphosphate Km: 0.00035 mM in the reaction with 4-methyl-2-oxopentanoate Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
3-methyl-2-oxobutanoate substrate inhibition Bos taurus
3-methylbutanoyl-CoA competitive with CoA Bos taurus
4-methyl-2-oxopentanoate substrate inhibition Bos taurus
D-3-methyl-2-oxopentanoate substrate inhibition Bos taurus
L-3-Methyl-2-oxopentanoate substrate inhibition Bos taurus
NADH competitive with NAD+ Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
4-methyl-2-oxopentanoate
-
Bos taurus
0.0156
-
3-methyl-2-oxobutanoate
-
Bos taurus
0.0172
-
DL-3-methyl-2-oxopentanoate
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bos taurus
Mg2+ Km: 0.0042 mM in the reaction with 4-methyl-2-oxopentanoate Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine
-
Bos taurus [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2
-
?
3-methyl-2-oxopentanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine D-3-methyl-2-oxopentanoate and L-3-methyl-2-oxopentanoate Bos taurus [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylbutanoyl)dihydrolipoyllysine + CO2
-
?
4-methyl-2-oxopentanoate + NAD+ + CoA
-
Bos taurus 3-methylbutanoyl-CoA + CO2 + NADH
-
?

Cofactor

Cofactor Comment Organism Structure
CoA Km: 0.0071 mM in the reaction with 4-methyl-2-oxopentanoate, Km: 0.009 mM in the reaction with 3-methyl-2-oxobutanoate, Km: 0.007 in the reaction with DL-3-methyl-2-oxopentanoate Bos taurus
NAD+ Km: 0.109 mM in the reaction with 4-methyl-2-oxopentanoate, Km: 0.126 mM in the reaction with 3-methyl-2-oxobutanoate, Km: 0.101 mM in the reaction with DL-3-methyl-2-oxobutanoate Bos taurus