Application | Comment | Organism |
---|---|---|
synthesis | expression of the structural genes for all three subunits of the Escherichia coli 2-oxoglutarate dehydrogenase complex in Saccharomyces cerevisiae. The Escherichia coli lipoic-acid scavenging enzyme is coexpressed to enable cytosolic lipoylation of the complex, which is required for its enzymatic activity. In vivo cytosolic activity of the complex is tested by constructing a reporter strain in which the essential metabolite 5-aminolevulinic acid can only be synthetized from cytosolic, and not mitochondrial, succinyl-CoA. In the resulting strain, complementation of 5-aminolevulinic acid auxotrophy depends on activation of the 2-oxoglutarate dehydrogenase complex by lipoic acid addition | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae cytosol | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM used in assay conditions | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | Escherichia coli | - |
[dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AFG3 | - |
- |
Escherichia coli | P0AFG3 | i.e. SucA, subunit E1 of the 2-oxoglutarate dehydrogenase complex | - |
Purification (Comment) | Organism |
---|---|
Sephacryl S-500 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | - |
Escherichia coli | [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-ketoglutarate dehydrogenase | - |
Escherichia coli |
alphaKGDH | - |
Escherichia coli |
SucA | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Escherichia coli |