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Literature summary for 1.2.4.2 extracted from
- Selective inhibition of 2-oxoglutarate and 2-oxoadipate dehydrogenases by the phosphonate analogs of their 2-oxo acid substrates (2021), Front. Chem., 8, 596187 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adipoyl phosphonate | - |
Rattus norvegicus | |
| glutaryl phosphonate | - |
Rattus norvegicus | |
| succinyl phosphonate | - |
Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
2-oxoadipate | preparation from liver, low affinity state, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.27 | - |
2-oxoglutarate | enzyme from liver, at pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 0.27 | - |
2-oxoadipate | preparation from liver, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.45 | - |
2-oxoglutarate | enzyme from heart, at pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 0.45 | - |
2-oxoadipate | preparation from heart, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.45 | - |
2-oxoadipate | preparation from liver, low affinity state, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.55 | - |
2-oxoadipate | preparation from heart, pH 7, temperature not specified in the publication | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM used in assay conditions | Rattus norvegicus | |
| Mg2+ | 1 mM used in assay conditions | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | Rattus norvegicus | - |
[dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | Q4KLP0 | - |
- |
| Rattus norvegicus | Q5XI78 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoadipate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | - |
Rattus norvegicus | [dihydrolipoyllysine-residue succinyltransferase] S-glutaryldihydrolipoyllysine + CO2 | - |
? | |
| 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | - |
Rattus norvegicus | [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-oxoadipate dehydrogenase | - |
Rattus norvegicus |
| 2-oxoglutarate dehydrogenase | - |
Rattus norvegicus |
| DHTKD1 | - |
Rattus norvegicus |
| E1o | - |
Rattus norvegicus |
| OADH | - |
Rattus norvegicus |
| OGDH | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00005 | - |
adipoyl phosphonate | and 0.50, preparation from liver, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.0014 | - |
glutaryl phosphonate | and 0.0024, preparation from liver, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.0024 | - |
glutaryl phosphonate | and 0.0014, preparation from liver, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.0038 | - |
succinyl phosphonate | and 0.128, preparation from liver, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.0038 | - |
glutaryl phosphonate | preparation from heart, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.0073 | - |
glutaryl phosphonate | enzyme from liver, at pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 0.0096 | - |
succinyl phosphonate | enzyme from liver, at pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 0.0096 | - |
succinyl phosphonate | preparation from liver, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.0102 | - |
succinyl phosphonate | preparation from heart, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.02 | - |
succinyl phosphonate | enzyme from heart, at pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 0.02 | - |
succinyl phosphonate | preparation from heart, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.039 | - |
glutaryl phosphonate | enzyme from heart, at pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 0.039 | - |
glutaryl phosphonate | preparation from heart, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.049 | - |
adipoyl phosphonate | preparation from heart, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.05 | - |
adipoyl phosphonate | and 0.000050, preparation from liver, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.073 | - |
glutaryl phosphonate | preparation from liver, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.128 | - |
succinyl phosphonate | and 0.0038, preparation from liver, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 0.405 | - |
adipoyl phosphonate | enzyme from liver, at pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 0.405 | - |
adipoyl phosphonate | preparation from liver, pH 7, temperature not specified in the publication | Rattus norvegicus | |
| 2.487 | - |
adipoyl phosphonate | enzyme from heart, at pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 2.487 | - |
adipoyl phosphonate | preparation from heart, pH 7, temperature not specified in the publication | Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | isoenzymes 2-oxoglutarate dehydrogenase (OGDH) and 2-oxoadipate dehydrogenase (OADH) show a 100fold difference in their ratio in the heart and liver, but similar Michaelis saturations by 2-oxoglutarate are inherent in the enzyme preparations from these tissues. In the heart, OADH/OGDH ratio is about 0.01, and OADH possesses low-affinity sites to 2-oxoadipate. In liver preparation, OADH/OGDH ratio is about 1.6, and OADH a biphasic saturation with 2-oxoadipate | Rattus norvegicus |
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Release 2023.1 (January 2023)
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