Literature summary for 1.2.4.2 extracted from

Lei, G.; Wang, X.; Lai, C.; Li, Z.M.; Zhang, W.; Xie, C.; Wang, F.; Wu, X.; Li, Z.
Expression and biochemical characterization of alpha-ketoglutarate decarboxylase from cyanobacterium Synechococcus sp. PCC7002 (2018), Int. J. Biol. Macromol., 114, 188-193 .

Search for more literature for 1.2.4.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Synechococcus sp. PCC 7002

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	40-50% inhibition	Synechococcus sp. PCC 7002
Cu2+	40-50% inhibition	Synechococcus sp. PCC 7002
Zn2+	2 mM, loss of activity	Synechococcus sp. PCC 7002

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.19	-	2-oxoglutarate	pH 7.0, 25°C	Synechococcus sp. PCC 7002

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	1.8-2.3fold stimulation	Synechococcus sp. PCC 7002
Mg2+	2.3fold stimulation	Synechococcus sp. PCC 7002
Mn2+	1.8-2.3fold stimulation	Synechococcus sp. PCC 7002

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
247000	-	gel filtration	Synechococcus sp. PCC 7002

Organism

Organism	UniProt	Comment	Textmining
Synechococcus sp. PCC 7002	B1XMM5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine	-	Synechococcus sp. PCC 7002	[dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2	-	?
additional information	no substrates: pyruvic acid, benzoylformic acid, 2-oxopentanoic acid, 4-methyl-2-oxopentanoic acid and 3-methyl-2-oxobutanoic acid. The enzyme additionally displays low acetolactate synthase activity	Synechococcus sp. PCC 7002	?	-	-

Subunits

Subunits	Comment	Organism
tetramer	4 * 60000, SDS-PAGE, 4 * 61667, calculated from sequence	Synechococcus sp. PCC 7002

Synonyms

Synonyms	Comment	Organism
ilvB	-	Synechococcus sp. PCC 7002

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Synechococcus sp. PCC 7002

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.2	-	2-oxoglutarate	pH 7.0, 25°C	Synechococcus sp. PCC 7002

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7	-	Synechococcus sp. PCC 7002

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	-	34% residual activity	Synechococcus sp. PCC 7002
8	-	17% residual activity	Synechococcus sp. PCC 7002

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Synechococcus sp. PCC 7002

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
6.3	-	2-oxoglutarate	pH 7.0, 25°C	Synechococcus sp. PCC 7002

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Release 2023.1 (January 2023)