Application | Comment | Organism |
---|---|---|
biotechnology | active expression of enzyme from non-halophilic Zymomonas mobilis in the haloarchaeon Haloferax volcanii with no difference in the secondary structure. Post-transcriptional mechanisms in the stationary phase appear to limit the amount of recombinant protein expressed | Zymomonas mobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | at 2 M, 50% residual activity of enzyme expressed in Escherichia coli | Zymomonas mobilis | |
NaCl | at 2 M, 50% residual activity of enzyme expressed in Escherichia coli | Zymomonas mobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, SDS-PAGE | Zymomonas mobilis |
240000 | - |
gel filtration, recombinant protein from Escherichia coli and from Haloferax volcanii | Zymomonas mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | - |
recombinant expression in Haloferax volcanii | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme both expressed in Escherichia coli and Haloferax volcanii | Zymomonas mobilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme from Haloferax volcanii | Zymomonas mobilis |
91 | - |
recombinant enzyme from Escherichia coli | Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + NAD+ | - |
Zymomonas mobilis | acetyl-CoA + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 60000, SDS-PAGE | Zymomonas mobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Zymomonas mobilis |